LOCUS       V027423                 7560 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027423
VERSION     V027423
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3707
                     /label="Musk(NM_001037127)"
                     /note="Musk(NM_001037127)"
                     /gene="Musk"
     CDS             3726..3755
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3774..3797
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3817..3833)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3844..4466
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4615..5203)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5532..5579
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5811..6605)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6640..6997)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6999..7103)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7130..7558
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gagagagctt gtcaacattc cactgttaca gatgctcacc ctggttgcct
     1081 tcagcgggac tgagaaactt ccaaaagccc ctgtcatcac cacgcctctt gaaactgtag
     1141 atgccttggt tgaagaagta gcgactttca tgtgtgccgt ggaatcctac cctcagcccg
     1201 agatttcttg gaccagaaat aaaattctca ttaagctgtt tgacacccgc tacagcatcc
     1261 gggagaatgg tcagctcctc accattctga gcgtggaaga cagtgatgat ggcatctact
     1321 gctgcatagc caacaatgga gtgggaggag ccgtggagag ttgtggtgcc ctgcaagtga
     1381 agatgaaacc taaaataact cgtcctccca ttaatgtaaa aataatagag ggattgaagg
     1441 cagttctgcc gtgcactacg atgggtaacc ccaaaccatc tgtgtcctgg atcaaggggg
     1501 acaatgctct cagggaaaat tccagaatcg cagttcttga atctgggagc ttaaggatcc
     1561 ataatgtgca aaaggaagat gcaggacagt accgctgtgt ggccaaaaac agcctgggca
     1621 cagcttactc caaactggtg aagctggaag tggaggaaga cagagaacct gagcaggacg
     1681 ctaaagtttt tgcaagaatc ctgcgtgctc ctgaatccca caatgtcacc tttggttcct
     1741 ttgtaaccct acgctgcaca gcaataggca tccctgtccc caccatcagc tggattgaaa
     1801 acggaaatgc tgtttcttca ggttccattc aagagagtgt gaaagaccga gtgattgact
     1861 caagactcca gctcttcatc acaaagccag gactctacac atgcatagct accaataagc
     1921 acggagaaaa gttcagtacc gcaaaggctg cagccactgt cagcatagca gtccctcctc
     1981 cgtggttttc tatggatact tctttcctat ggacagaatg gagtaagtca cagaaagaca
     2041 gccaaggcta ctgtgcccag tacagagggg aggtgtgtga tgcagtcctg gcgaaagatg
     2101 ctcttgtctt cttcaacacc tcctaccggg accccgagga cgcccaggag ctgctgatcc
     2161 acactgcgtg gaatgagctg aaggctgtga gtccactgtg ccggccagct gctgaggctc
     2221 tgctgtgtaa ccacctcttc caagagtgca gccctggagt ggtacctact cccatgccca
     2281 tttgcagaga gtactgcctg gcggtaaagg agctcttctg tgcaaaggaa tggcaggcaa
     2341 tggaaggaaa ggcccaccgg ggcctctaca gatctgggat gcatctcctt ccggtaccag
     2401 agtgcagcaa gcttcccagc atgcaccggg accccacagc ctgcacaaga ctgccatatt
     2461 tagattataa aaaagaaaac ataacaacat tcccgtcaat aacgtcctcc aggccgagcg
     2521 cggacattcc aaacctgcct gcctccacct cttcctttgc cgtctcgcct gcgtactcca
     2581 tgaccgtcat catctccatc gtgtccagct ttgccctgtt tgctcttctc accatcgcta
     2641 ctctctattg ctgccgaagg aggaaagaat ggaaaaataa gaaaagagag tcgaccgcgg
     2701 tgaccctcac cacgttgcct tccgagctcc tgctggatag gctccatccc aaccccatgt
     2761 accagaggat gccactcctt ctgaatccta agttgctcag cctggagtat ccgaggaata
     2821 acattgagta tgtccgagac atcggagagg gggcgtttgg aagagtcttc caagcaaggg
     2881 cccctggctt gctgccttat gaacctttca ctatggtggc cgtgaagatg cttaaggaag
     2941 aggcctctgc agacatgcaa gcggactttc agagggaggc ggccctcatg gcagagtttg
     3001 acaaccccaa cattgtgaaa ctcttaggtg tgtgtgccgt tgggaagccg atgtgtctgc
     3061 tctttgaata tatggcctat ggtgacctca atgagttcct ccgaagtatg tccccgcaca
     3121 ctgtttgcag cctcagccac agtgacctgt ccacgagggc tcgggtgtct agccctggtc
     3181 ctccaccact gtcctgtgca gaacagctct gcattgccag gcaggtggca gctggcatgg
     3241 cctacctttc agagcgcaag tttgtccacc gggacttagc taccaggaac tgcctggttg
     3301 gggagaccat ggtggtgaaa attgcagact ttggcctctc caggaacatc tattccgcag
     3361 actactacaa agctgatgga aatgacgcca tccctatccg ctggatgccg cccgagtcta
     3421 tcttctacaa ccgctacacc actgagtcgg atgtatgggc ctatggtgtg gtcctctggg
     3481 agatcttctc ctatgggctg cagccctact atggaatggc ccacgaggag gtcatttact
     3541 atgtgagaga tggcaacatc ctcgcctgcc ctgagaactg ccccttggaa ctgtacaacc
     3601 tcatgcgcct gtgttggagc aagctgcctg ctgatagacc cagcttctgc agtatccaca
     3661 ggatcctgca gcgcatgtgc gagagagcag agggaacggt gggtgtcacg cgtacgcggc
     3721 cgctcgagca gaaactcatc tcagaagagg atctggcagc aaatgatatc ctggattaca
     3781 aggatgacga cgataaggtt taaacggccg gccgcggtca tagctgtttc ctgaacagat
     3841 cccgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     3901 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     3961 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     4021 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     4081 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     4141 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     4201 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     4261 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     4321 ctgattttaa aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc
     4381 catgcccaac cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg
     4441 tccactcagt agatgcctgt tgaattgggt acgcggccag cggcgagcgg tatcagctca
     4501 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4561 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4621 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4681 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4741 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4801 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4861 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4921 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4981 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5041 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5101 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5161 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5221 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5281 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5341 ctaaagtata tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc
     5401 gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc
     5461 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac
     5521 cccgcgttta tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt
     5581 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg
     5641 gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg
     5701 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
     5761 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
     5821 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     5881 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     5941 gctatgtcct gatagcgatc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     6001 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
     6061 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     6121 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     6181 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     6241 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     6301 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     6361 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     6421 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     6481 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     6541 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     6601 atcatgcgaa acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc
     6661 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
     6721 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
     6781 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct
     6841 ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt
     6901 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa
     6961 ctgacacaca ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat
     7021 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7081 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     7141 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7201 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7261 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7321 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7381 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7441 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7501 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
//
