LOCUS       V030746                 6204 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030746
VERSION     V030746
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2351
                     /label="Mepe(NM_053172)"
                     /note="Mepe(NM_053172)"
                     /gene="Mepe"
     CDS             2370..2399
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2418..2441
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2461..2477)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2488..3110
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3259..3847)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4176..4223
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4455..5249)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5284..5641)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5643..5747)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5774..6202
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gacgccagag ggcctcatga agatgcaggc tgtgtctgtt ggactgctcc
     1081 tcttcagtat gacctgggcg gcaccaatgc cgaatgaaga caggagcagc tgcggcaatc
     1141 aagacagcat tcacaaggac ttggcagcat ctgtgtatcc tgatcccacg gtggatgaag
     1201 gcacagagga tgggcaaggt gctctccttc acccgcctgg ccaggacagg tatggtgctg
     1261 ccctcctcag aaatatcacg cagcctgtaa agagtctagt gactggggcc gaactacgga
     1321 gggaaggaaa ccaggagaag agacctcaga gtgttctaag cgtaattcca gcagatgtca
     1381 atgatgctaa agtctcctta aaagacataa agaatcaaga gagttatctg ctaacccaga
     1441 gcagcccggt caaaagcaaa cacaccaaac acacccgcca gacccgacgg agcactcact
     1501 acctgacaca tctcccacag atcaagaaga ctcccagtga ccttgaaggc agtggctccc
     1561 cagatcttct agtgagggga gataatgatg tccccccttt cagtggagat gggcaacatt
     1621 ttatgcacat tcctggcaaa ggaggtgctg ggtctggtcc tgaaagctca actagtcgcc
     1681 ccctctcagg ctccagcaaa gctgaagtta ttgacccaca tatgagtgga ctaggctcta
     1741 atgagatccc ggggagagaa ggacatggtg gcagtgccta tgcaaccaga gacaaagctg
     1801 cacagggggc aggctctgca ggtgggagcc ttgtgggggg cagcaatgaa atcacaggca
     1861 gcaccaattt cagggaactc cccggaaaag aaggaaacag aattaatgcc ggcagccaaa
     1921 atgctcatca agggaaagta gaatttcact atccacaagt ggcctcgaga gaaaaggtaa
     1981 aggggggcgt ggagcatgca gggagagctg gttacaacga aatccccaag agcagcaaag
     2041 gtagctctag caaagatgca gaagagtcca aagggaacca attaaccttg actgcaagcc
     2101 aaagatttcc aggtaaaggc aaaagccagg gccctgctct gccctctcac agtcttagta
     2161 atgaggttaa aagtgaagaa aaccattatg tgttccatgg acaaaataat cttacaccga
     2221 ataaagggat gtcacagcgg agaggctcct ggccttcgag aagacccaat tcccacaggc
     2281 gcgctagcac ccgccaaaga gacagcagcg agtcgtcatc cagtgggagt tctagtgaga
     2341 gtcatggtga cacgcgtacg cggccgctcg agcagaaact catctcagaa gaggatctgg
     2401 cagcaaatga tatcctggat tacaaggatg acgacgataa ggtttaaacg gccggccgcg
     2461 gtcatagctg tttcctgaac agatcccggg tggcatccct gtgacccctc cccagtgcct
     2521 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     2581 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     2641 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     2701 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     2761 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     2821 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     2881 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     2941 accactgctc ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc
     3001 cagacacagc ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt
     3061 ggcactgtcc tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg
     3121 ccagcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3181 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3241 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3301 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3361 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3421 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3481 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3541 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3601 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3661 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3721 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3781 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3841 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3901 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3961 ttaaaaatga agttttaaat caatctaaag tatatatgag taacctgagg ctatggcagg
     4021 gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg gggggtgggg
     4081 tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg gggtatcgac
     4141 agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa caccgtgcgt
     4201 tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg
     4261 tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat ccccgcgctg
     4321 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     4381 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     4441 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     4501 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     4561 tcagcaatat cacgggtagc caacgctatg tcctgatagc gatccgccac acccagccgg
     4621 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     4681 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac
     4741 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     4801 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     4861 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     4921 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     4981 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     5041 agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc
     5101 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     5161 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     5221 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg
     5281 atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     5341 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     5401 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     5461 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     5521 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     5581 gcctggggac tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca
     5641 ggactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5701 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5761 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5821 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5881 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5941 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6001 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6061 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6121 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6181 taacaaaaat ttaacgcgaa tttt
//
