LOCUS       V031034                 6993 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031034
VERSION     V031034
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3140
                     /label="Masp1(NM_008555)"
                     /note="Masp1(NM_008555)"
                     /gene="Masp1"
     CDS             3159..3188
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3207..3230
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3250..3266)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3277..3899
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4048..4636)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4965..5012
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5244..6038)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6073..6430)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6432..6536)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6563..6991
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaggttcctg tctttctggc ggctcctcct ctaccacgct ctgtgcctcg
     1081 ccctgccgga ggtttcagcc cataccgtgg agctaaacga aatgtttggt cagatccagt
     1141 cacctggcta tccagattcc tatccaagtg actctgaggt gacatggaat attactgtcc
     1201 cggaggggtt tcgaatcaag ctttacttca tgcacttcaa cttggaatcc tcctatcttt
     1261 gtgaatacga ctatgtgaag gtagaaacag aagaccaggt gctggcaacc ttttgtggca
     1321 gggagaccac cgatactgag cagacccccg gccaggaagt ggttctttcg cctggcacct
     1381 tcatgtctgt cactttccgg tcagatttct ccaatgagga acgattcaca ggcttcgacg
     1441 cccactacat ggctgtagat gtggatgagt gcaaggagag ggaagatgaa gagctgtcct
     1501 gtgaccacta ctgtcacaac tacatcggtg gctactactg ctcctgccgc tttggctaca
     1561 tcctccacac agacaacagg acctgccgag tggaatgcag cggcaatctc tttacccaga
     1621 ggacaggcac aatcaccagc cccgattacc ccaaccctta tcccaagagc tcagaatgtt
     1681 cctataccat tgacctggag gaaggcttca tggtcagcct gcagtttgag gacatttttg
     1741 acattgaaga ccatcctgag gtgccctgtc cctatgacta cattaagatt aaagctggtt
     1801 caaaagtatg gggtcccttc tgtggagaga aatccccaga accaatcagc acccagactc
     1861 acagtgtcca gatcctattc cgcagcgaca actcaggaga gaaccgaggc tggaggctct
     1921 cctacagagc ggcaggaaat gagtgcccaa agctacagcc tcctgtgtac gggaaaatcg
     1981 agccctcgca ggccgtgtat tccttcaaag accaagtgct cgtcagctgt gacacaggct
     2041 acaaagtgct aaaggataac gaggtgatgg acacattcca aattgagtgt ctgaaggacg
     2101 gtgcatggag taacaagatc cccacctgta aaattgtaga ctgtggagct cctgcagggc
     2161 tgaaacatgg gctagtaacc ttctccacca gaaacaacct caccacatac aaatctgaga
     2221 taaggtactc ctgccaacag ccctattaca agatgcttca caataccaca ggtgtatata
     2281 cgtgttctgc tcatgggacc tggacgaacg aagtgctcaa gagaagcctg cccacctgcc
     2341 ttccagtgtg tggtgtcccc aagttctccc ggaagcagat ctccaggatc ttcaatggcc
     2401 gcccagccca gaagggtacc atgccatgga ttgccatgct gtcacacctg aacggacaac
     2461 ccttctgtgg gggtagcctt ttaggttcca actgggtttt gacagctgct cactgcctcc
     2521 accagtcact tgatccagaa gaaccaaccc tacacagctc atacttgctc agcccttctg
     2581 acttcaaaat tatcatggga aagcactgga gacggcgctc agacgaagac gagcagcacc
     2641 tgcatgtaaa gcgcaccacg ctccacccac tgtacaaccc cagcacgttt gagaacgacc
     2701 ttggtctggt ggaactgtca gagagcccga ggctgaacga ctttgtgatg cctgtctgtc
     2761 tgcctgagca gccttccact gaaggaacca tggtcatcgt cagtggctgg gggaagcagt
     2821 tcttacagag gtttccagag aacctgatgg agattgaaat cccaattgta aactctgaca
     2881 cctgccagga ggcctatacc ccattgaaga agaaagtgac caaggacatg atctgtgccg
     2941 gagaaaagga aggggggaaa gatgcctgtg ctggtgactc tggaggccct atggtgacca
     3001 aagatgcaga gagagaccaa tggtacctgg tgggcgtggt gtcctggggt gaagattgcg
     3061 ggaagaaaga tcgctatgga gtctattctt acatctatcc caacaaggac tggatccaga
     3121 ggatcactgg ggtgaggaac acgcgtacgc ggccgctcga gcagaaactc atctcagaag
     3181 aggatctggc agcaaatgat atcctggatt acaaggatga cgacgataag gtttaaacgg
     3241 ccggccgcgg tcatagctgt ttcctgaaca gatcccgggt ggcatccctg tgacccctcc
     3301 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     3361 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     3421 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     3481 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     3541 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     3601 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     3661 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     3721 caggcgtgaa ccactgctcc cttccctgtc cttctgattt taaaataact ataccagcag
     3781 gaggacgtcc agacacagca taggctacct ggccatgccc aaccggtggg acatttgagt
     3841 tgcttgcttg gcactgtcct ctcatgcgtt gggtccactc agtagatgcc tgttgaattg
     3901 ggtacgcggc cagcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3961 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4021 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4081 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4141 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4201 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4261 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4321 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4381 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4441 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4501 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4561 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4621 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4681 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4741 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aacctgaggc
     4801 tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg
     4861 ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg
     4921 ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac
     4981 accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
     5041 ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca gcatgagatc
     5101 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     5161 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     5221 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     5281 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     5341 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg atccgccaca
     5401 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     5461 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc
     5521 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     5581 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     5641 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     5701 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     5761 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     5821 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac
     5881 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     5941 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     6001 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     6061 tcttgatcga tctttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     6121 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     6181 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
     6241 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     6301 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     6361 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gctggttctt
     6421 tccgcctcag gactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6481 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     6541 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6601 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6661 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6721 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6781 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6841 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6901 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6961 gagctgattt aacaaaaatt taacgcgaat ttt
//
