LOCUS       V035817                 9383 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035817
VERSION     V035817
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             775..1428
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
                     /label="GST"
     CDS             1465..4089
                     /label="MIOS(NM_019005)"
                     /note="MIOS(NM_019005)"
                     /gene="MIOS"
     misc_feature    4159..4747
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4813..4861
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5063..5491
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5505..5834
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5901..6695
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6871..6992
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7041..7057)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7065..7081
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7089..7119)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7134..7155
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7443..8031)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8202..9062)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9063..9167)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagc tagcgtttaa acttaagctt ggtaccgagc tcggatccgc caccatgtcc
      781 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa
      841 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga
      901 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat
      961 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg
     1021 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt
     1081 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt
     1141 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat
     1201 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt
     1261 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt
     1321 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct
     1381 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaagg aggaggggga
     1441 agcggcggcg gagggagtga attcatgagc ggtaccaaac ctgatatttt atgggcacca
     1501 caccatgttg atagatttgt tgtgtgtgac tcagaactaa gtctttatca tgtggaatct
     1561 actgtgaatt cagaactcaa agctggatct ttacgtttat ctgaagactc tgcagctaca
     1621 ttactgtcaa taaattcaga tacaccctat atgaaatgtg ttgcctggta tcttaattat
     1681 gatcctgaat gtctgctggc agttggacaa gcaaatggtc gagttgtact tacaagcctt
     1741 ggtcaagatc ataactcaaa gttcaaagat ttgataggaa aagagtttgt tccaaaacat
     1801 gcacgacaat gtaataccct tgcctggaat ccactggata gtaactggct agctgctggt
     1861 ttagataagc acagagctga cttttcagtg ctaatatggg atatctgcag caaatatact
     1921 cctgatatag ttcccatgga aaaagtgaaa ctttcagcag gtgaaactga aacaacatta
     1981 ttagtaacaa aaccacttta tgagttagga cagaatgatg cttgtctgtc tctttgttgg
     2041 cttccacgag accagaaact tctccttgct ggtatgcatc gtaacctagc tatatttgat
     2101 cttcggaata caagccaaaa gatgttcgta aatacaaaag ctgttcaggg tgtgacggta
     2161 gacccatatt tccacgatcg tgttgcttcc ttctatgaag gtcaggttgc aatatgggat
     2221 cttagaaaat ttgagaagcc agttttgaca ttgactgagc aaccaaaacc cttaacaaaa
     2281 gtagcatggt gtcccactag gactggtcta cttgccactt taacaaggga tagtaatatt
     2341 attagattgt atgatatgca gcatacaccc actcccattg gggatgaaac tgaacccaca
     2401 ataattgaaa gaagtgtgca accttgtgac aattacattg cttcctttgc gtggcatcca
     2461 acaagtcaaa atcgaatgat agttgtaact cccaaccgaa caatgtcaga cttcactgtt
     2521 tttgaaagga tatctcttgc ctggagccca attacatctt taatgtgggc ttgtggtcgt
     2581 catttatatg aatgtacgga agaagaaaat gataattctt tagaaaaaga tatagcaacg
     2641 aagatgcgtc ttcgggcttt atcaaggtat ggacttgata cagagcaggt gtggaggaac
     2701 cacattttag ctggaaatga agatccacag ctcaagtcac tctggtatac tctgcacttt
     2761 atgaagcaat acacagaaga tatggatcag aaatctccag gcaacaaagg atcattggtt
     2821 tatgcaggaa ttaaatcaat tgtaaagtca tcgttgggaa tggtggaaag cagcagacat
     2881 aattggagtg ggttggataa gcaaagtgat attcaaaatt taaatgaaga gagaatctta
     2941 gctttacagc tttgtgggtg gataaagaaa ggaacggatg tagacgtggg gccatttttg
     3001 aactcccttg tacaagaagg ggaatgggaa agagctgctg ctgtggcatt gttcaacttg
     3061 gatattcgcc gagcaatcca aatcctgaat gaaggggcat cttctgaaaa aggagatctg
     3121 aatctcaatg tggtagcaat ggctttatcg ggttatacgg atgagaagaa ctccctttgg
     3181 agagaaatgt gtagcacact gcgattacag ctaaataacc cgtatttgtg tgtcatgttt
     3241 gcatttctga caagtgaaac aggatcttac gatggagttt tgtatgaaaa caaagttgca
     3301 gtacgtgaca gagtggcatt tgcttgtaaa ttccttagtg atactcagtt aaatagatac
     3361 atcgaaaagt tgaccaatga aatgaaagag gctggaaatt tggaaggaat tttgcttaca
     3421 ggccttacta aagatggagt ggacttaatg gagagttatg ttgatagaac tggagatgtt
     3481 caaacagcaa gttactgtat gttacagggt tcacctttag atgttcttaa agatgaaagg
     3541 gttcagtact ggattgagaa ttatagaaat ttattagatg cctggaggtt ttggcataaa
     3601 cgagctgaat ttgatattca caggagtaag ttggatccca gttccaagcc tttagcacaa
     3661 gtttttgtga gttgcaattt ctgtggcaag tcaatctcct acagctgttc agctgtgcct
     3721 catcagggca gaggttttag tcagtatggt gtgagtggct caccaacgaa atctaaagtc
     3781 acaagttgtc ctggctgtcg aaaaccactt cctcgatgtg cgctttgtct cattaatatg
     3841 ggaacaccag tttctagctg tcctggagga accaaatcag atgaaaaagt ggacttgagc
     3901 aaggacaaaa aattagccca atttaacaac tggtttacat ggtgtcataa ttgcaggcac
     3961 ggtggacatg ctggacatat gcttagttgg ttcagggacc atgcagagtg ccctgtgtcg
     4021 gcatgcacgt gtaaatgtat gcagttggat acaacaggga atctggtacc tgcagagact
     4081 gtccagccat aactcgagtc tagagggccc cttaagggtt cgatccctac cggttagtaa
     4141 tgagtttgat atctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4201 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4261 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4321 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4381 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4441 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4501 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     4561 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4621 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4681 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4741 tccccgcctg gaaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     4801 cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt
     4861 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     4921 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     4981 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc
     5041 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5101 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5161 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc
     5221 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5281 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5341 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5401 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat
     5461 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     5521 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5581 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5641 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5701 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5761 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5821 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     5881 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     5941 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6001 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6061 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     6121 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6181 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6241 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6301 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6361 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6421 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     6481 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     6541 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     6601 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     6661 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg
     6721 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6781 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6841 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6901 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6961 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     7021 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7081 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7141 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7201 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7261 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7321 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7381 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7441 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7501 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7561 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7621 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7681 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7741 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7801 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7861 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7921 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7981 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8041 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8101 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8161 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8221 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8281 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8341 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8401 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8461 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8521 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8581 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8641 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8701 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8761 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8821 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8881 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8941 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9001 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9061 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9121 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9181 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca
     9241 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     9301 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     9361 attgcatgaa gaatctgctt agg
//
