LOCUS       V040829                11436 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040829
VERSION     V040829
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..6116
                     /label="Ltbp2(NM_021586)"
                     /note="Ltbp2(NM_021586)"
                     /gene="Ltbp2"
     CDS             6123..6188
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6212..6800
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    6866..6914
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7116..7544
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7558..7887
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7954..8748
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8924..9045
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9094..9110)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9118..9134
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9142..9172)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9187..9208
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9496..10084)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10255..11115)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11116..11220)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgga gagcacctcc ctgcgaggtc
      781 tccggtgccc acagctctgc agccactctg gcgccatgag ggcgccgacc accgtccgct
      841 gctccggacg catccaaagg gcgcgttgga ggggcttcct gccacttgtc ctggctctct
      901 tgatggggac aagtcatgcc caaagggatt ccgtggggag atacgaacca gctagccggg
      961 atgccaatcg gttgtggcgc cccgtgggca accaccccgc agcggctgca gccaaggtgt
     1021 acagtctgtt ccgagagccc gacgcgccgg tccccggctt gtcgccctct gagtggaatc
     1081 agccgggcca ggggatccct gggaggctcg cagaggccga ggccaggaga ccgtcccgag
     1141 cccagcagct gcgtcgagtc cagtcacctg tccagactcg gagaagcaat ccccgaggcc
     1201 agcagccacc agcagcccgg accgcacatt ccgtcgtgcg cctggcgacc cctcagcgac
     1261 ccgcggctgc acgccgaggg cggctcaccg ggagaaatgt ctgcggggga cagtgctgcc
     1321 ctggatggac gacatcgaac agcaccaacc actgtatcaa acctgtgtgt cagcctccct
     1381 gtcagaaccg gggctcctgc agccggcccc agctctgcat ctgccgttct ggcttccgtg
     1441 gggcacgctg cgaggaggtc atccctgagg aggagtttga ccctcagaat gccaggcctg
     1501 tgcccagacg ctcagtggag ggagcacctg gccctcacag gagcagcgag gccagaggaa
     1561 gtctagtgac cagaatacag ccgctgctac caccactacc accacctcca tctaggaccc
     1621 tcagccagac ccgtcccctg cagcagcatg caggactgtc cagaacagtt cgtcgttatc
     1681 cggccactgg taccaatggc caactgatgt ccaacgctct gccttcagga ccaggacctg
     1741 agctgagaga cagcagccaa caggcagcac acatgaacca tctctcacac ccctgggggc
     1801 tgaacctcac cgagaaaatc aagaagatta aggtcgtctt cactcccacc atctgcaagc
     1861 agacctgtgc ccggggccgc tgtgccaaca cgtgtgagaa gggtgacacc accaccctgt
     1921 acagtcaggg cggccatggg catgacccca agtctggctt ccgtatctat ttctgccaaa
     1981 tcccctgcct gaatggaggc cgctgcattg gccgggacga gtgctggtgt ccagccaact
     2041 ctacagggaa gttctgccat ctgcctgtcc cacagccaga cagggagcct ccaggacgag
     2101 gctcccagca cagagccctg ctggaagggc cattgaagca atccaccttc acgctgcctc
     2161 tctccaacca gctggcctct gtgaacccct cgctggtgaa ggtacaaatg cagcacccgc
     2221 ctgaggcctc cgtgcagatc caccaggtgg cccgggtccg gggtgaggtg gaccctgtgc
     2281 cagaggacaa cagtgtggag accagagcct ctcatcgccc ccatggcagc tcaggccaca
     2341 gccactgggc cagcaacagc atacccgctc gggctggaga ggcccctcgg ccaccaccag
     2401 tgccgtccag gcattatgga cttctgggcc agtgttacct gagcacggtg aatggacagt
     2461 gtgctaaccc cctaggggag ctgacttctc aggaagactg ctgtggcagt gtggggactt
     2521 cttggggggt gacttcctgt gccccatgcc cacccagacc agctttcccc gtgattgaaa
     2581 acggccagct ggagtgtccc caagggtata agagactaaa cctcagccat tgccaagaca
     2641 tcaatgagtg cctgaccctg ggcctgtgca aggattcaga gtgtgtgaac accaggggca
     2701 gctacctgtg cacctgcagg cccggcctca tgctggatcc atcaaggagc cgctgtgtat
     2761 cggacaaggc tgtctccatg aaacagggac tctgttaccg gtcaatggtg tctggcacct
     2821 gcaccctgcc tttggtacaa cggatcacca agcagatatg ctgttgcagc cgtgtgggca
     2881 aagcctgggg cagcaaatgt gaacactgcc ccctgcctgg cacagaagcc ttcagggaga
     2941 tctgccctgc tggccatggc tacgcctact caagctcaga catccgcctg tctatgagga
     3001 aagctgagga agaggaactg gctagccccg taagggaaca gagacagcag agcagtggac
     3061 ccccacctgg ggcagcagaa aggcagccac tccgggcagc cactgccacc tggattgagg
     3121 ctgagaccct ccctgacaaa ggtgactctc gggctattca gattacaacc agtgctcccc
     3181 acctacctgc ccgggtacca ggggatgcca ctggaagacc aacgccatca ttgcctggac
     3241 agggcattcc agagggtcca gcagaagagc aggtgatccc ttccagtgat gtcctggtga
     3301 cgcacggtcc cccaggcttt gatccatgtt tcgctggagc ctccaacatc tgtggccctg
     3361 ggacctgtgt gaagctccca aatggataca gatgtgtctg cagccctggt taccagctac
     3421 accccagcca ggactactgt actgatgaca acgagtgtct gaggaacccc tgtgaaggaa
     3481 gagggcgctg tgtcaacagt gtgggctcct actcctgcct ctgctaccca ggctacacac
     3541 tagccaccct aggagacaca caggagtgcc aagatgtgga tgagtgtgag cagccggggg
     3601 tgtgcagcgg tggacgatgc agcaacactg agggctcgta ccactgcgag tgtgatcagg
     3661 gctacgtcat ggtcagaaga ggacactgcc aagatatcaa cgaatgccgt caccctggta
     3721 cctgccctga tgggagatgc gtcaactccc ctggctccta cacttgtctg gcctgtgagg
     3781 agggctacat agggcagagc gggaactgtg tagatatgaa tgagtgtctg acccccggga
     3841 tatgtgccca tggaaggtgc atcaacatgg aaggctcctt tagatgctct tgtgagccag
     3901 gctatgagct caccccagac aagaagggct gccgagatgt ggacgagtgt gccagccgag
     3961 cctcatgccc caccggcctc tgcctcaaca cggagggctc cttcacctgc tcagcctgtc
     4021 agagtgggta ctgggtgaac gaagatggca ctgcctgtga agacctggat gaatgtgcct
     4081 tccccggagt ctgccccaca ggcgtctgca ccaacactgt gggctccttc tcctgcaagg
     4141 actgcgacag gggcttccgg cccagccccc tgggcaacag ctgtgaagat gtggatgagt
     4201 gtgaaggtcc ccagaacagc tgcctgggag gcgagtgcaa gaacacagat ggttcctacc
     4261 agtgcctctg tccccagggc ttccagctgg ccaatggcac cgtgtgtgag gatgtggacg
     4321 aatgtgttgg ggaagaacac tgcgctcctc atggcgaatg cctcaacagc ccggggtcct
     4381 tcttctgtct ctgtgcaccc ggctttgcta gtgctgaggg gggcaccaga tgccaggatg
     4441 ttgatgaatg tgcaaccaca gagccgtgtc tgggaggaca ctgtgtcaac accgagggct
     4501 ccttcaactg tctgtgtgag actggcttcc agcccgcccc agacagtgga gagtgtgtgg
     4561 acatagatga atgtgcaaat gatactgtgt gtgggaacca tggcttctgt gacaatacgg
     4621 atggctcctt ccgctgcctg tgtgaccagg gcttcgagac ctcaccctca ggctgggagt
     4681 gtgttgatgt gaacgagtgt gagctcatgc tggcagtgtg tggggatgca ctctgcgaga
     4741 acgtggaagg ctccttcctg tgcctttgtg ccagtgacct tgaggagtat gatgcagaag
     4801 aaggacactg ccgtcctcgg gtggctggag ctcagagaat cccagaggtc ccaacagagg
     4861 agcaggctgc aggccttacc ggcatggagt gctatgctga acacaatggt ggtcctccat
     4921 gctctcaaat cttgggccag aactccacac aggctgagtg ctgctcgacc cagggtgcca
     4981 gatgggggga aacctgtgat ccctgcccat ctgaggactc agttgaattc agtgagctgt
     5041 gccccagtgg tcaaggttac atcccagtgg aaggggcctg gacatttgga caagccatgt
     5101 atacagatgc cgacgagtgc atactgtttg ggcctgctct ctgccagaat ggccgatgcc
     5161 tcaacacagt gcctggctac atttgcctgt gcaaccctgg ctaccactat gatgccgtca
     5221 gcaggaagtg ccaggatcac aacgaatgcc aggacttggc ctgtgagaac ggcgagtgtg
     5281 tgaacacaga aggctccttc cactgcttct gcagtccccc cctcatccta gacctcagcg
     5341 gacagcgctg tgtgaacagt accagcagct cagaggactt ccctgaccat gacatccaca
     5401 tggacatctg ctggaaaaaa gtcaccaatg acgtgtgcag ccagcccttg cgtgggcacc
     5461 atactaccta tacagagtgc tgctgccaag acggggaggc ctggagccag cagtgtgctc
     5521 tgtgcccccc caggagctct gaggtctatg ctcagctgtg caatgtggct cggattgagg
     5581 cagagaggga agcagggatc cacttccggc caggatatga gtatggccct ggcccagatg
     5641 atctacctga aaccctctac ggcccagatg gagccccttt ctataactac ctgggccctg
     5701 aggacactgt tcctgagcct cccttctcca acacagccag tcatttggga gacaacacac
     5761 ccatccttga gcctcctctg cagccctctg aacttcagcc ccactatatc cccagccatt
     5821 cagaacccct ggcttccttc gaaggccttc aggctgagga atgtggcatc ctgaatggct
     5881 gtgagaatgg ccgctgtgtg cgtgtgcgcg agggctacac ttgtgactgc tttgaaggct
     5941 tccagctgga tacagccctc atggcctgtg tggatgtgaa tgagtgtgaa gacctgaacg
     6001 ggcctgcggc actctgtgcg catggtcact gcgagaacac agagggttcc tatcgctgcc
     6061 actgttcccc tggttacgtg gcagagcccg ggcccccaca ctgtgcagcc aaggagggat
     6121 ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg
     6181 atgacaagta gtgagggccc gatatctcga caatcaacct ctggattaca aaatttgtga
     6241 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6301 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6361 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6421 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6481 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6541 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6601 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6661 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6721 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6781 cctttgggcc gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg gaaggagaca
     6841 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt
     6901 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     6961 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     7021 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     7081 agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     7141 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     7201 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     7261 tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     7321 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     7381 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7441 actcaaccct atctcggtct attcttttga tttataaggg attttgggga tttcggccta
     7501 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     7561 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7621 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7681 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7741 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7801 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     7861 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     7921 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     7981 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     8041 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     8101 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     8161 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     8221 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     8281 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     8341 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     8401 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     8461 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     8521 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     8581 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     8641 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     8701 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     8761 ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     8821 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     8881 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     8941 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9001 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     9061 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     9121 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     9181 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     9241 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9301 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9361 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9421 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9481 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9541 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9601 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9661 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     9721 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9781 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9841 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9901 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9961 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    10021 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    10081 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    10141 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    10201 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    10261 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    10321 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10381 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10441 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10501 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10561 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10621 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10681 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10741 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10801 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10861 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10921 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10981 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    11041 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    11101 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    11161 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    11221 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
    11281 tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
    11341 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
    11401 ggcttgaccg acaattgcat gaagaatctg cttagg
//
