LOCUS       V030943                 5961 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030943
VERSION     V030943
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2108
                     /label="Ltb4r2(NM_020490)"
                     /note="Ltb4r2(NM_020490)"
                     /gene="Ltb4r2"
     CDS             2127..2156
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2175..2198
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2218..2234)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2245..2867
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3016..3604)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3933..3980
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4212..5006)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5041..5398)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5400..5504)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5531..5959
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gtctgtctgc taccgtccgc ctgggaatga gacactactg agctggaagg
     1081 gctcgcgggc caccggcact gcctttctac tgctggcggc gttgctggga ctgccaggca
     1141 atggcttcgt agtatggagc ttagcgggct ggcggcccac ggcgggccgg ccactggcgg
     1201 ccacacttgt gctgcacctg gccctggccg acggcgcggt gctgctgctc acgccgctct
     1261 ttgtggcttt cctcagccaa gaggcttggc ccttgggcca ggtgggctgc aaggcggtgt
     1321 actacgtgtg cgcgctcagc atgtacgcca gcgtgctgct caccggcctg ctcagcctgc
     1381 agcgctgcct ggctgtcact cggcccttcc tggctccccg actgcgcagc ccggccctgg
     1441 cccgccgctt gctgctgggg gtctggctgg ccgccctggt gctcgccgtc ccggccgcgg
     1501 tctaccgcca cctctggggc gggcgcgtgt gtcagctgtg ccacccatcg ccagtgcacg
     1561 cagctgctca tctgagcctg gagaccctga ctgccttcgt cttgcctttt gggaccgtgc
     1621 tcggctgcta tggcgtgacg ctggcgcggt tgcgaggagc gcgctggggc tcggggcgac
     1681 aaggcacacg ggtgggtcgt ctggtgagcg ccatcgtgct ggcctttggc ttgctctggg
     1741 ctccctacca cgcggtcaat ctcctacagg cggtggccgc gctcgctccg ccggaaggac
     1801 ccctggccag gctcggcggg gcgggccagg cggcgcgcgc tggaactaca gccttggctt
     1861 tcttcagttc cagcgtcaac ccagtgctct atgtcttcac tgcgggggat ttgctgccgc
     1921 gggccgggcc tcggttcctt actcgactct tcgagggctc tggggaggcc cgagggggca
     1981 gccgctctag ggagggtacc atggaactcc gaactactcc taagctgaaa gtaatggggc
     2041 agggcagggg caatggagac cctggaggcg gagatggggg caagacggag aaagacagtc
     2101 aagaatggac gcgtacgcgg ccgctcgagc agaaactcat ctcagaagag gatctggcag
     2161 caaatgatat cctggattac aaggatgacg acgataaggt ttaaacggcc ggccgcggtc
     2221 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc
     2281 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     2341 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     2401 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     2461 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     2521 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     2581 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     2641 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     2701 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag
     2761 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc
     2821 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca
     2881 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2941 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3001 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3061 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3181 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3421 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3541 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3601 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3661 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3721 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc
     3781 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
     3841 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
     3901 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
     3961 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
     4021 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     4081 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     4141 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
     4201 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     4261 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     4321 gcaatatcac gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca
     4381 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     4441 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
     4501 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     4561 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     4621 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     4681 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     4741 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     4801 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg
     4861 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     4921 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     4981 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc
     5041 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     5101 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     5161 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     5221 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     5281 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     5341 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagga
     5401 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5461 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5521 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5581 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5641 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5701 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5761 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5821 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5881 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5941 caaaaattta acgcgaattt t
//
