LOCUS       V030977                 6303 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030977
VERSION     V030977
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2450
                     /label="Lpl(NM_008509)"
                     /note="Lpl(NM_008509)"
                     /gene="Lpl"
     CDS             2469..2498
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2517..2540
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2560..2576)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2587..3209
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3358..3946)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4275..4322
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4554..5348)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5383..5740)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5742..5846)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5873..6301
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagagcaaa gccctgctcc tggtggtcct gggagtttgg ctccagagtt
     1081 tgaccgcctt ccgaggaggg gtggccgcag cagacgcggg aagagatttc tcagacatcg
     1141 aaagcaaatt tgccctaagg acccctgaag acacagctga ggacacttgt catctcattc
     1201 ctggattagc agactctgtg tctaactgcc acttcaacca cagcagcaag accttcgtgg
     1261 tgatccatgg atggacggta acgggaatgt atgagagttg ggtgcccaaa cttgtggccg
     1321 ccctgtacaa gagagaacct gactccaatg tcattgtagt agactggttg tatcgggccc
     1381 agcaacatta tccagtgtca gctggctaca ccaagctggt gggaaatgat gtggccagat
     1441 tcatcaactg gatggaggag gagtttaact accccctaga caacgtccac ctcttagggt
     1501 acagccttgg agcccatgct gctggcgtag caggaagtct gaccaataag aaggtcaata
     1561 gaattactgg tttggatcca gctgggccta actttgagta tgcagaagcc cccagtcgcc
     1621 tttctcctga tgacgctgat tttgtagatg tcttacacac atttaccagg gggtcacctg
     1681 gtcgaagtat tggaatccag aaaccagtag ggcatgttga catttatccc aatggaggca
     1741 ctttccagcc aggatgcaac attggagaag ccatccgtgt gattgcagag agaggactcg
     1801 gagacgtgga ccagctggtg aagtgctcgc acgagcgctc cattcatctc ttcattgact
     1861 ccctgctgaa tgaagaaaac cccagcaagg catacaggtg caactccaag gaagcctttg
     1921 agaaagggct ctgcctgagt tgtagaaaga atcgctgtaa caatctgggc tatgagatca
     1981 acaaggtcag agccaagaga agcagcaaga tgtacctgaa gactcgctct cagatgccct
     2041 acaaagtgtt ccattaccaa gtcaagattc acttttctgg gactgaggat ggcaagcaac
     2101 acaaccaggc cttcgagatt tctctgtacg gcacagtggc cgagagcgag aacattccct
     2161 tcaccctgcc cgaggtttcc acaaataaaa cctactcctt cttgatttac acggaggtgg
     2221 acatcggaga actgctcatg atgaagctta agtggataag cgactcctac ttcagctggc
     2281 ctgactggtg gagcagcccc agcttcgtca tcgagaggat ccgagtgaaa gccggagaga
     2341 ctcagaaaaa ggtcatcttc tgtgctaggg agaaagtttc tcatctgcag aagggaaagg
     2401 actcagcagt gtttgtgaaa tgccatgaca agtctctgaa gaagtctggc acgcgtacgc
     2461 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt
     2521 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca
     2581 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     2641 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     2701 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     2761 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     2821 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     2881 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     2941 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     3001 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     3061 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct
     3121 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt
     3181 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc
     3241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3781 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4081 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc
     4141 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg
     4201 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg
     4261 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc
     4321 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct
     4381 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     4441 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     4501 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
     4561 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     4621 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     4681 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa
     4741 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     4801 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     4861 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     4921 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     4981 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     5041 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     5101 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     5161 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     5221 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     5281 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     5341 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc
     5401 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     5461 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     5521 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     5581 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     5641 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     5701 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat
     5761 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5821 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     5881 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5941 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6001 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6061 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6121 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6181 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6241 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6301 ttt
//
