LOCUS       V030972                 6483 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030972
VERSION     V030972
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2630
                     /label="Lpcat1(NM_145376)"
                     /note="Lpcat1(NM_145376)"
                     /gene="Lpcat1"
     CDS             2649..2678
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2697..2720
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2740..2756)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2767..3389
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3538..4126)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4455..4502
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4734..5528)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5563..5920)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5922..6026)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6053..6481
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaggctgcgg ggccgcgggc cgcgggccgc cccctcctcc agctctgggg
     1081 caggcgacgc gcggcgccta gcgcccccgg ggcggaaccc cttcgtgcac gagctgcgac
     1141 tgagcgccct gcagaaggcc caggtggctt tcatgacgtt gacgctgttc cccatccggc
     1201 tcctgttcgc tgctttcatg atgctgctgg cctggccctt tgcactcctg gcttccctgg
     1261 gacctcctga taaggagcca gagcagcccc tggccttatg gaggaaggtc gtggacttcc
     1321 tgctcaaggc catcatgcgc accatgtggt ttgctggcgg cttccaccgt gtagctgtga
     1381 aggggcggca ggccctgcct actgaggctg ctatcctcac cttggctcca cattcctcct
     1441 actttgatgc catcccggtc accatgacca tgtcctccat tgtgatgaag gcagagagca
     1501 gagacatccc aatctgggga actctgataa gatacatcag gccagtgttc gtgtcccgct
     1561 ctgaccagga ctcgcgaagg aagacagtgg aggagatcaa gcgacgggca cagtcgaatg
     1621 gaaagtggcc tcagataatg atttttccag aaggaacttg tacaaatagg acctgcctca
     1681 ttaccttcaa acctggtgcg ttcattcctg gagttcctgt ccagcccgtg gtgctacgct
     1741 acccaaacaa attggacacc atcacatgga cgtggcaagg acctggagcg ttgaaaatcc
     1801 tgtggctcac tctgtgccag tttcaaaacc aagtggaaat tgaatttctg cctgtgtatt
     1861 gcccttctga agaggagaag aggaatcctg ccctgtatgc cagcaatgtg aggcgtgtca
     1921 tggccaaggc cctgggtgtc tcagtaaccg actatacatt tgaggactgc cagctggctc
     1981 tggcagaagg acaacttcgc ttgcctgctg acacctgcct gctagagttt gccaggcttg
     2041 tgaggggcct cggactaaaa ccagaaaatc ttgaaaaaga tctagacaaa tattcagaaa
     2101 gtgcgaggat gaagagagga gagaaaatcc gccttccaga gtttgcggcc tacttggaag
     2161 ttcctgtctc agatgcactg gaagacatgt tctcgctttt tgacgagagt ggtggtggtg
     2221 agattgacct tcgtgaatat gtggtcgcct tgtctgtggt gtgcaggcca tcccagacct
     2281 tagccaccat ccagctggca ttcaagatgt acggatctcc tgaggatggc agcatagatg
     2341 aggccaacct gtcctgcatc ctcaagactg cactgggtgt atcagaatta acagtgaccg
     2401 acttgttcca ggctattgac caagaggaca agggaagaat cacctttgat gacttctgcg
     2461 ggtttgcgga aatgtacccc gactacgcag aggactactt gtaccctgat cagacacatt
     2521 tcgacagctg tgcacagaca cccccagcac caactcccaa tggcttctgc attgacttca
     2581 gccctgagaa ctcagacttt gggagaaaga attcttgtaa gaaagcggac acgcgtacgc
     2641 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt
     2701 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca
     2761 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     2821 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     2881 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     2941 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     3001 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     3061 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     3121 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     3181 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     3241 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct
     3301 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt
     3361 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc
     3421 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3481 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3541 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3601 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3661 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3721 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3781 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3841 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3901 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3961 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4021 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4081 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4141 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4201 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4261 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc
     4321 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg
     4381 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg
     4441 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc
     4501 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct
     4561 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     4621 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     4681 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
     4741 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     4801 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     4861 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa
     4921 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     4981 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     5041 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     5101 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     5161 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     5221 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     5281 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     5341 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     5401 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     5461 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     5521 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc
     5581 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     5641 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     5701 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     5761 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     5821 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     5881 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat
     5941 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6001 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     6061 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6121 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6181 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6241 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6301 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6361 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6421 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6481 ttt
//
