LOCUS       V031245                 5973 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031245
VERSION     V031245
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2120
                     /label="Lpar1(NM_001421016)"
                     /note="Lpar1(NM_001421016)"
                     /gene="Lpar1"
     CDS             2139..2168
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2187..2210
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2230..2246)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2257..2879
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3028..3616)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3945..3992
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4224..5018)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5053..5410)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5412..5516)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5543..5971
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcagctgcc tctacttcca gccctgtaat ttcacagccc cagttcacag
     1081 ccatgaacga acaacagtgc ttctacaatg agtctatcgc cttcttttat aaccggagtg
     1141 ggaaatatct agccacagaa tggaacacag tgagcaagct ggtgatggga ctgggcatca
     1201 ctgtttgcgt gttcatcatg ttggccaatc tcctggtcat ggtggcaatc tacgtcaacc
     1261 gccgcttcca tttccctatt tattacttga tggccaacct ggctgctgca gacttcttcg
     1321 ctggattggc ctacttctac ctgatgttca atacaggacc taatacccgg agactgactg
     1381 ttagcacgtg gctcctccgg cagggcctca ttgacaccag cctgacagct tctgtggcca
     1441 acctgctggc tattgctatc gagaggcaca tcacggtttt ccgcatgcag ctccatacac
     1501 gaatgagcaa ccggcgcgtg gtggtggtga ttgtagtcat ctggactatg gccattgtga
     1561 tgggtgctat acccagtgtg ggctggaact gcatctgtga tatcgatcac tgttccaaca
     1621 tggcacccct ctacagtgac tcctacttag tcttctgggc cattttcaac ctggtgacct
     1681 ttgtggtcat ggtggttctc tacgctcaca tctttggcta tgttcgccag aggactatga
     1741 ggatgtctcg gcatagttct ggacccagga ggaatcggga caccatgatg agccttctga
     1801 agactgtggt cattgtgctt ggtgccttta ttgtctgctg gactccggga ttggtcttgt
     1861 tattgctgga tgtgtgctgc ccgcagtgcg atgtcctggc ctatgagaag ttcttcctcc
     1921 tcctggccga gttcaactct gctatgaacc ccatcatcta ctcctaccgc gacaaagaga
     1981 tgagcgccac cttcaggcag atcctgtgtt gccagcgcaa cgagaaccct aatggcccca
     2041 cggaaggctc tgaccgctct gcctcctccc tcaaccacac cattctggct ggagttcaca
     2101 gcaacgacca ctctgtggtt acgcgtacgc ggccgctcga gcagaaactc atctcagaag
     2161 aggatctggc agcaaatgat atcctggatt acaaggatga cgacgataag gtttaaacgg
     2221 ccggccgcgg tcatagctgt ttcctgaaca gatcccgggt ggcatccctg tgacccctcc
     2281 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     2341 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     2401 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     2461 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     2521 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     2581 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     2641 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     2701 caggcgtgaa ccactgctcc cttccctgtc cttctgattt taaaataact ataccagcag
     2761 gaggacgtcc agacacagca taggctacct ggccatgccc aaccggtggg acatttgagt
     2821 tgcttgcttg gcactgtcct ctcatgcgtt gggtccactc agtagatgcc tgttgaattg
     2881 ggtacgcggc cagcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     2941 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     3481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aacctgaggc
     3781 tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg
     3841 ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg
     3901 ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac
     3961 accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
     4021 ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca gcatgagatc
     4081 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     4141 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     4201 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     4261 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     4321 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg atccgccaca
     4381 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     4441 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc
     4501 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     4561 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     4621 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     4681 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     4741 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     4801 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac
     4861 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     4921 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     4981 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     5041 tcttgatcga tctttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     5101 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     5161 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
     5221 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     5281 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     5341 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gctggttctt
     5401 tccgcctcag gactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5461 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5521 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5581 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5641 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5701 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5761 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5821 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5881 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5941 gagctgattt aacaaaaatt taacgcgaat ttt
//
