LOCUS pEnCMV-LBR(human)-HA 7891 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026360 VERSION V026360 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..2550 /label="LBR(NM_002296)" /note="LBR(NM_002296)" /gene="LBR" CDS 2557..2583 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2587..2613 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2617..2643 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 2667..3255 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3321..3369 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3571..3999 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4013..4342 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4409..5203 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5379..5500 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5549..5565) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5573..5589 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5597..5627) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5642..5663 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5951..6539) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6710..7570) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7571..7675) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgcc aagtaggaaa 721 tttgccgatg gtgaagtggt aagaggtcga tggcctggga gttcacttta ttatgaagta 781 gaaattctga gccacgacag cacctcccag ctttacactg tgaagtataa agatggaaca 841 gagcttgaat tgaaagagaa tgatattaag cctttaactt cctttaggca aaggaaaggt 901 ggctcaactt ccagttcccc ttccagacgc cgagggagtc gatcaaggtc acgctcccga 961 tcccctggtc gaccacctaa aagtgcccgc cgatctgctt ctgcttccca ccaggccgac 1021 attaaggaag caaggaggga agtggaagtt aaattgactc cgctgattct gaagccattt 1081 ggaaatagca tcagcagata taatggggag cctgagcata ttgagagaaa tgacgcacct 1141 cataaaaata cacaggaaaa attcagtttg tcacaagaaa gcagttacat agcaacacag 1201 tatagccttc gtccaagaag agaagaagtc aaattaaaag aaatagattc taaggaagaa 1261 aaatacgttg caaaagaact ggcagtgaga acctttgaag tgacccccat ccgggcaaag 1321 gacttggagt ttggaggagt acctggtgtg tttctcatca tgtttggcct gcctgtgttc 1381 ctcttcctgt tgctgttgat gtgtaaacag aaagatccca gtcttctgaa tttccctcct 1441 cctttgccag ctttgtatga gttatgggaa accagagtat ttggggtcta cctcctgtgg 1501 tttttgattc aagtcctgtt ctacctactg ccaattggaa aggttgtaga aggaacgcct 1561 cttattgatg gaagaagact caagtataga ttaaatggat tctatgcttt tatcctgaca 1621 tctgcagtca tcggaacatc tctcttccag ggcgtagagt ttcattacgt gtacagtcat 1681 tttcttcagt ttgcacttgc ggccactgtt ttttgtgtgg tcttgagtgt gtatctctac 1741 atgcgctctt tgaaagcgcc ccggaatgac ctgtcgcctg ccagctctgg aaatgctgtc 1801 tatgatttct tcattggccg tgaattaaac cctcgaattg gtacttttga tctcaaatac 1861 ttttgtgaat tgcgccccgg attgattgga tgggtggtta ttaacttggt gatgcttttg 1921 gctgaaatga aaatacagga ccgcgctgtt ccatccttgg ccatgatttt agttaatagt 1981 ttccagcttc tctatgtggt ggatgctctc tggaatgagg aagcgttgtt gacgaccatg 2041 gacatcatcc acgatggatt tggattcatg ctggcttttg gagacttggt gtgggttccc 2101 tttatttaca gcttccaagc cttttattta gtcagtcatc caaatgaagt gtcttggcca 2161 atggcttctc taattattgt tctgaaactt tgtggttatg taatcttccg aggtgcaaat 2221 tctcagaaaa atgcattccg gaaaaatccc agtgatccaa agcttgcaca tttaaaaacc 2281 attcatactt caacgggaaa aaatcttcta gtttctggat ggtggggctt tgttcgccac 2341 cccaattact tgggtgatct catcatggcc ttggcgtggt ccctcccatg tggttttaac 2401 cacattctgc cttatttcta cataatttat ttcaccatgt tgcttgtcca ccgagaagct 2461 cgtgacgagt accactgtaa gaagaaatac ggcgtggctt gggaaaagta ctgtcagcgt 2521 gtgccctacc gtatatttcc atacatctac ctcgagtacc catacgacgt acctgattat 2581 gcaacttatc cttatgatgt acctgattat gctagctatc cttatgatgt cccagactac 2641 gcatagtgag ggcccgatat ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat 2701 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 2761 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 2821 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 2881 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 2941 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 3001 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 3061 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 3121 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 3181 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 3241 gggccgcctc cccgcctgga aacgggggag gctaactgaa acacggaagg agacaatacc 3301 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt 3361 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 3421 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt 3481 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc 3541 tgcgcagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 3601 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 3661 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 3721 atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 3781 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 3841 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 3901 accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt 3961 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 4021 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 4081 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 4141 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 4201 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 4261 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 4321 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 4381 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 4441 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 4501 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 4561 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 4621 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 4681 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 4741 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 4801 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 4861 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 4921 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 4981 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 5041 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 5101 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 5161 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 5221 gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 5281 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 5341 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata 5401 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 5461 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga 5521 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 5581 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 5641 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 5701 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 5761 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 5821 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 5881 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 5941 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6001 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6061 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6121 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg 6181 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6241 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 6301 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 6361 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 6421 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 6481 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 6541 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 6601 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 6661 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 6721 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 6781 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 6841 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 6901 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 6961 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7021 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7081 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7141 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7201 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7261 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 7321 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 7381 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 7441 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 7501 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 7561 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 7621 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 7681 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtc 7741 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg 7801 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt 7861 gaccgacaat tgcatgaaga atctgcttag g //