LOCUS V030041 7609 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030041 VERSION V030041 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..2268 /label="Kpna4(NM_008467)" /note="Kpna4(NM_008467)" /gene="Kpna4" CDS 2275..2301 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2305..2331 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2335..2361 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 2385..2973 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3039..3087 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3289..3717 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3731..4060 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4127..4921 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5097..5218 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5267..5283) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5291..5307 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5315..5345) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5360..5381 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5669..6257) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6428..7288) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7289..7393) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc ggacaacgag 721 aaattggaca accaacggct caagaatttt aagaacaaag gccgtgactt ggagactatg 781 agaagacaac gaaatgaagt tgtagttgaa ttaaggaaga ataaaagaga tgaacatctc 841 ttaaagagaa ggaatgtacc acaagaagat atctgtgaag actccgatat agatggtgat 901 tatagagtgc aaaatacctc tctagaagct attgttcaaa atgcttcaag tgataaccaa 961 ggaattcaat taagtgcagt tcaagctgct aggaagcttt tatccagtga tcgaaatcca 1021 ccaattgatg acttaataaa atctggaata ttgcctattt tagttcattg tcttgaaaga 1081 gatgacaatc cttctttaca gtttgaagct gcgtgggctt tgacaaacat tgcatctgga 1141 acgtctgaac aaacgcaagc agtggttcag tccaatgctg tgccactttt cttgaggctt 1201 ctgcattcac cccatcagaa tgtctgtgaa caggcagtat gggcgttggg aaacatcata 1261 ggtgatggac ctcagtgtag agattatgtg ataagtcttg gagttgtaaa acctctgctt 1321 tcattcataa gtccatctat tcctataaca ttcttaagaa atgttacctg ggttatggtc 1381 aatttatgtc gccacaaaga cccaccacca ccaatggaga caattcagga gattcttcca 1441 gctctttgtg tattaattca tcacacagat gtaaatatcc tggtagatac tgtctgggcc 1501 ctgtcttacc ttacggatgc tggcaatgaa cagatacaga tggtaataga ctctggcata 1561 gttcctcact tggttccgct actcagccac caggaagtta aagttcagac tgctgcactg 1621 cgagctgtgg gcaacattgt cactggaact gatgagcaaa cacaagtagt tttaaactgt 1681 gatgctctct cacacttccc agcactcctc actcatccca aagagaaaat taataaagaa 1741 gcagtgtggt tcctctccaa catcactgca ggaaatcaac agcaggttca ggcagtaatt 1801 gatgccaatc ttgtaccaat gataatacac cttttggata agggagattt tggcactcag 1861 aaagaagctg cctgggccat aagtaactta acaatcagcg gaaggaaaga ccaagtggcc 1921 tatcttatcc aacaaaatgt tattccacct ttttgcaatt tgctgactgt aaaagatgca 1981 caagttgtgc aagtagtact tgatggacta agtaatatat taaaaatggc tgaagatcaa 2041 gcagaaacca tagccaatct catagaagaa tgtggtggac tggagaaaat tgaacaactt 2101 cagaatcatg aaaatgagga catctacaaa ttggcctatg agatcattga ccagttcttc 2161 tcttcagatg atattgatga agatcctagc cttgttccag agtcagttca aggtggaaca 2221 tttggtttca attcatcgac caatgtacca acagaggggt tccagtttct cgagtaccca 2281 tacgacgtac ctgattatgc aacttatcct tatgatgtac ctgattatgc tagctatcct 2341 tatgatgtcc cagactacgc atagtgaggg cccgatatct cgacaatcaa cctctggatt 2401 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 2461 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 2521 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 2581 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 2641 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 2701 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 2761 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 2821 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 2881 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 2941 cgagtcggat ctccctttgg gccgcctccc cgcctggaaa cgggggaggc taactgaaac 3001 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa 3061 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact 3121 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc 3181 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag 3241 gccctgccat agcagatctg cgcagctggg gctctagggg gtatccccac gcgccctgta 3301 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 3361 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 3421 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc 3481 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 3541 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 3601 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg 3661 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 3721 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 3781 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 3841 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 3901 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 3961 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 4021 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 4081 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 4141 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 4201 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 4261 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 4321 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 4381 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 4441 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 4501 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 4561 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 4621 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 4681 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 4741 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 4801 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 4861 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 4921 agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 4981 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 5041 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact 5101 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 5161 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 5221 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 5281 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 5341 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 5401 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 5461 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 5521 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 5581 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 5641 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 5701 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 5761 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 5821 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt 5881 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 5941 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 6001 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 6061 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 6121 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 6181 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 6241 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 6301 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 6361 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 6421 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 6481 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 6541 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 6601 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 6661 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 6721 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 6781 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 6841 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 6901 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 6961 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 7021 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 7081 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 7141 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 7201 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 7261 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 7321 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 7381 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 7441 tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca 7501 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc 7561 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttagg //