LOCUS       V031095                 5679 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031095
VERSION     V031095
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1826
                     /label="Klra3(NM_001289605)"
                     /note="Klra3(NM_001289605)"
                     /gene="Klra3"
     CDS             1845..1874
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1893..1916
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1936..1952)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1963..2585
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2734..3322)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3651..3698
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3930..4724)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4759..5116)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5118..5222)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5249..5677
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gagtgagcca gaggtcactt actcaactgt gagacttcat aagtcttcag
     1081 ggttgcagaa actagtaagg catgaggaga ctcaagggcc cagagaagtt ggcaacagaa
     1141 agtgttcagc accctggcaa ctcattgtga aagctcttgg aatcctctgt ttccttcttc
     1201 ttgtaacagt tgcagtgttg gcagtaaaga tttttcagta taatcaacac aaacaagaaa
     1261 tcaatgaaac tctaaaccac caccataact gcagcaacat gcaaagggct ttcaacttaa
     1321 aggaagaaat gttgacaaat aagtctatag attgtaggcc aagcaatgaa actctggaat
     1381 atatcaaaag agaacaggac agatgggaca gtaaaacaaa gactgtttta gattcctcac
     1441 gggacacagg cagaggtgtt aaatactggt tctgctatag tactaaatgt tattatttca
     1501 tcatgaacaa aacgacatgg agtggatgta aagcgaactg ccagcattat agcgttccca
     1561 ttctgaagat agaagatgaa gatgaactga aattccttca acgccatgtt attccagaga
     1621 attactggat tggattgtct tatgataaga aaaaaaagga atgggcatgg attgacaatg
     1681 gcccatctaa acttgacatg aaaataagga aaatgaactt taagtctaga ggatgtgtat
     1741 ttttatctaa agcaagaata gaagatattg actgtaatat tccctactac tgtatttgtg
     1801 ggaagaaact ggataaattc cctgatacgc gtacgcggcc gctcgagcag aaactcatct
     1861 cagaagagga tctggcagca aatgatatcc tggattacaa ggatgacgac gataaggttt
     1921 aaacggccgg ccgcggtcat agctgtttcc tgaacagatc ccgggtggca tccctgtgac
     1981 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     2041 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     2101 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     2161 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     2221 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     2281 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     2341 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     2401 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttaaa ataactatac
     2461 cagcaggagg acgtccagac acagcatagg ctacctggcc atgcccaacc ggtgggacat
     2521 ttgagttgct tgcttggcac tgtcctctca tgcgttgggt ccactcagta gatgcctgtt
     2581 gaattgggta cgcggccagc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2641 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2701 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2761 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2821 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2881 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2941 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3001 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3061 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3361 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3421 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaacc
     3481 tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
     3541 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
     3601 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
     3661 cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
     3721 tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga actccagcat
     3781 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     3841 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     3901 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     3961 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     4021 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcgatcc
     4081 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     4141 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc
     4201 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     4261 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     4321 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     4381 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     4441 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     4501 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca
     4561 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     4621 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     4681 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     4741 cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     4801 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     4861 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     4921 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     4981 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     5041 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     5101 gttctttccg cctcaggact cttccttttt caatattatt gaagcattta tcagggttat
     5161 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5221 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     5281 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5341 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5401 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5461 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5521 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5581 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5641 aaaaatgagc tgatttaaca aaaatttaac gcgaatttt
//
