LOCUS V030739 7923 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030739 VERSION V030739 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..4070 /label="Kl(NM_013823)" /note="Kl(NM_013823)" /gene="Kl" CDS 4089..4118 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 4137..4160 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(4180..4196) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 4207..4829 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(4978..5566) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 5895..5942 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(6174..6968) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(7003..7360) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(7362..7466) /gene="bla" /label="AmpR promoter" rep_origin 7493..7921 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gctagcccgc gcccctcctc gccgcccgcc gcggctggtg ctgctccgtt 1081 tgctgttgct gcatctgctg ctgctcgccc tgcgcgcccg ctgcctgagc gctgagccgg 1141 gtcagggcgc gcagacctgg gctcgcttcg cgcgcgctcc tgccccagag gccgctggcc 1201 tcctccacga caccttcccc gacggtttcc tctgggcggt aggcagcgcc gcctatcaga 1261 ccgagggcgg ctggcgacag cacggcaaag gcgcgtccat ctgggacact ttcacccatc 1321 actctggggc ggccccgtcc gactccccga tcgtcgtggc gccgtcgggt gccccgtcgc 1381 ctcccctgtc ctccactgga gatgtggcca gcgatagtta caacaacgtc taccgcgaca 1441 cagaggggct gcgcgaactg ggggtcaccc actaccgctt ctccatatcg tgggcgcggg 1501 tgctccccaa tggcaccgcg ggcactccca accgcgaggg gctgcgctac taccggcggc 1561 tgctggagcg gctgcgggag ctgggcgtgc agccggtggt taccctgtac cattgggacc 1621 tgccacagcg cctgcaggac acctatggcg gatgggccaa tcgcgccctg gccgaccatt 1681 tcagggatta tgccgagctc tgcttccgcc acttcggtgg tcaggtcaag tactggatca 1741 ccattgacaa cccctacgtg gtggcctggc acgggtatgc caccgggcgc ctggccccgg 1801 gcgtgagggg cagctccagg ctcgggtacc tggttgccca caacctactt ttggctcatg 1861 ccaaagtctg gcatctctac aacacctctt tccgccccac acagggaggc cgggtgtcta 1921 tcgccttaag ctcccattgg atcaatcctc gaagaatgac tgactataat atcagagaat 1981 gccagaagtc tcttgacttt gtgctaggct ggtttgccaa acccatattt attgatggcg 2041 actacccaga gagtatgaag aacaacctct cgtctcttct gcctgatttt actgaatctg 2101 agaagaggct catcagagga actgctgact tttttgctct ctccttcgga ccaaccttga 2161 gctttcagct attggaccct aacatgaagt tccgccaatt ggagtctccc aacctgaggc 2221 agcttctgtc ttggatagat ctggaatata accaccctcc aatatttatt gtggaaaatg 2281 gctggtttgt ctcgggaacc accaaaaggg atgatgccaa atatatgtat tatctcaaga 2341 agttcataat ggaaacctta aaagcaatca gactggatgg ggtcgacgtc attgggtaca 2401 ccgcgtggtc gctcatggac ggtttcgagt ggcatagggg ctacagcatc cggcgaggac 2461 tcttctacgt tgactttctg agtcaggaca aggagctgtt gccaaagtct tcggccttgt 2521 tctaccaaaa gctgatagag gacaatggct ttcctccttt acctgaaaac cagccccttg 2581 aagggacatt tccctgtgac tttgcttggg gagttgttga caactacgtt caagtggaca 2641 ctactctctc tcagtttact gacccgaatg tctatctgtg ggatgtgcat cacagtaaga 2701 ggcttattaa agtagacggg gttgtagcca agaagagaaa accttactgt gttgatttct 2761 ctgccatccg gcctcagata accttacttc gagaaatgcg ggtcacccac tttcgcttct 2821 ccctggactg ggccctgatc ttgcctctgg gtaaccagac ccaagtgaac cacacggttc 2881 tgcacttcta ccgctgcatg atcagcgagc tggtgcacgc caacatcact ccagtggtgg 2941 ccctgtggca gccagcagcc ccgcaccaag gcctgccaca tgcccttgca aaacatgggg 3001 cctgggagaa cccgcacact gctctggcgt ttgcagacta cgcaaacctg tgttttaaag 3061 agttgggtca ctgggtcaat ctctggatca ccatgaacga gccaaacaca cggaacatga 3121 cctatcgtgc cgggcaccac ctcctgagag cccatgcctt ggcttggcat ctgtacgatg 3181 acaagtttag ggcggctcag aaaggcaaaa tatccatcgc cttgcaggct gactggatag 3241 aaccggcctg ccctttctct caaaatgaca aagaagtggc cgagagagtt ttggaatttg 3301 atataggctg gctggcagag cctatttttg gttccggaga ttatccacgt gtgatgaggg 3361 actggctgaa ccaaaaaaac aattttcttt tgccctattt caccgaagat gaaaaaaagc 3421 tagtccgggg ttcctttgac ttcctggcgg tgagtcatta caccaccatt ctggtagact 3481 gggaaaagga ggatccgatg aaatacaacg attacttgga ggtacaggag atgactgaca 3541 tcacatggct caactctccc agtcaggtgg cagtggtgcc ttgggggctg cgcaaagtgc 3601 tcaactggct aaggttcaag tacggagacc tcccgatgta tgtgacagcc aatggaatcg 3661 atgatgaccc ccacgccgag caagactcac tgaggatcta ttatattaag aattatgtga 3721 atgaggctct gaaagcctac gtgttggacg acatcaacct ttgtggctac tttgcgtatt 3781 cacttagtga tcgctcagct cccaagtctg gcttttatcg atatgctgcg aatcagtttg 3841 agcccaaacc atctatgaaa cattacagga aaattattga cagcaatggc ttcctgggtt 3901 ctggaacact gggaaggttt tgtccagaag aatacactgt gtgcaccgaa tgtggatttt 3961 ttcaaacccg gaagtctttg ctggtcttca tctcgtttct tgtttttact tttattattt 4021 ctcttgctct catttttcac tactccaaga aaggccagag aagttataag acgcgtacgc 4081 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt 4141 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca 4201 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc 4261 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag 4321 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg 4381 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa 4441 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc 4501 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag 4561 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac 4621 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc 4681 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct 4741 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt 4801 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc 4861 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 4921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 4981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 5041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 5101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 5161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 5221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 5281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 5341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 5401 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 5461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 5521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 5581 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 5641 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 5701 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc 5761 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg 5821 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg 5881 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc 5941 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct 6001 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc 6061 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg 6121 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag 6181 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa 6241 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc 6301 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa 6361 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga 6421 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc 6481 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct 6541 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc 6601 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac 6661 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca 6721 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc 6781 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc 6841 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag 6901 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt 6961 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc 7021 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc 7081 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt 7141 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat 7201 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta 7261 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc 7321 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat 7381 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 7441 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc 7501 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 7561 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 7621 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 7681 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 7741 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 7801 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 7861 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 7921 ttt //