LOCUS       V030880                 6384 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030880
VERSION     V030880
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2531
                     /label="Kcnj3(NM_001355118)"
                     /note="Kcnj3(NM_001355118)"
                     /gene="Kcnj3"
     CDS             2550..2579
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2598..2621
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2641..2657)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2668..3290
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3439..4027)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4356..4403
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4635..5429)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5464..5821)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5823..5927)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5954..6382
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gtctgcactc cgaaggaaat ttggggacga ttaccaggta gtgaccactt
     1081 cgtcgagcgg ttcgggctta cagcctcagg ggccaggaca gggcccgcag cagcagctgg
     1141 tgcccaagaa gaaacggcag cggttcgtgg acaagaacgg ccggtgcaat gtgcagcatg
     1201 gtaacctggg cagcgagacc agtcgctacc tttcggacct cttcactacc ctggtggatc
     1261 tcaagtggcg ttggaacctc tttatcttca tcctcaccta caccgtggcc tggctcttca
     1321 tggcgtccat gtggtgggtg atcgcttata cccggggcga cctgaacaaa gcccatgtcg
     1381 gcaactacac tccctgtgtg gccaatgtct ataacttccc ctctgccttc ctcttcttca
     1441 tcgagaccga ggccaccatc ggctatggct accgctacat cacagataag tgccccgagg
     1501 gcatcatcct cttcctcttc cagtccatcc ttggctccat cgtggacgct ttcctcatcg
     1561 gctgcatgtt catcaagatg tcccagccca aaaagcgcgc cgagaccctc atgtttagcg
     1621 agcatgcggt tatctccatg agggacggaa aactcactct catgttccgg gtgggcaacc
     1681 tgcgcaacag ccacatggtc tccgcgcaga tccgctgcaa gctgctcaaa tctcggcaga
     1741 cacctgaggg tgagttcctt ccccttgacc aacttgaact ggatgtaggt tttagtacag
     1801 gggccgatca actttttctt gtgtcccctc tcacaatttg ccacgtgatc gatgccaaaa
     1861 gccccttcta tgacctatcc cagcgaagca tgcaaactga acagttcgag gttgtcgtca
     1921 tcctggaagg cattgtggaa accacaggaa tgacttgtca agctcgaaca tcatacacag
     1981 aggacgaagt gctttggggt catcgttttt tccctgtaat ttctttagaa gaaggattct
     2041 ttaaagtcga ttactcccag ttccatgcaa cctttgaagt tccaacccct ccttacagcg
     2101 tgaaagagca ggaggaaatg cttctcatgt cctccccttt aatagcacca gccataacca
     2161 acagcaaaga aagacacaat tctgtggagt gtttagatgg actagatgac attagcacaa
     2221 aacttccatc gaagctgcag aaaattacgg ggagagaaga ctttcccaaa aaacttctga
     2281 gaatgagttc tacaacctca gaaaaagcct atagtttggg tgatttgccc atgaaactcc
     2341 aacgaataag ttcagttcct ggcaactcgg aagaaaaact ggtttctaaa accaccaaga
     2401 tgttatctga tcccatgagc cagtctgtgg ccgatttgcc accaaagctt caaaagatgg
     2461 ctggaggacc taccaggatg gaaggaaatc ttccagccaa actaagaaaa atgaactctg
     2521 accgtttcac aacgcgtacg cggccgctcg agcagaaact catctcagaa gaggatctgg
     2581 cagcaaatga tatcctggat tacaaggatg acgacgataa ggtttaaacg gccggccgcg
     2641 gtcatagctg tttcctgaac agatcccggg tggcatccct gtgacccctc cccagtgcct
     2701 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     2761 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     2821 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     2881 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     2941 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     3001 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     3061 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     3121 accactgctc ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc
     3181 cagacacagc ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt
     3241 ggcactgtcc tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg
     3301 ccagcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3361 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3421 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3481 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3541 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3601 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3661 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3721 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3781 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3841 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3901 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3961 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4021 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4081 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4141 ttaaaaatga agttttaaat caatctaaag tatatatgag taacctgagg ctatggcagg
     4201 gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg gggggtgggg
     4261 tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg gggtatcgac
     4321 agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa caccgtgcgt
     4381 tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg
     4441 tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat ccccgcgctg
     4501 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     4561 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     4621 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     4681 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     4741 tcagcaatat cacgggtagc caacgctatg tcctgatagc gatccgccac acccagccgg
     4801 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     4861 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac
     4921 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     4981 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     5041 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     5101 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     5161 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     5221 agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc
     5281 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     5341 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     5401 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg
     5461 atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     5521 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     5581 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     5641 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     5701 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     5761 gcctggggac tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca
     5821 ggactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5881 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5941 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6001 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6061 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6121 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6181 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6241 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6301 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6361 taacaaaaat ttaacgcgaa tttt
//
