LOCUS       V037597                10910 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037597
VERSION     V037597
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             739..4806
                     /label="KDR(NM_002253)"
                     /note="KDR(NM_002253)"
                     /gene="KDR"
     CDS             4843..5559
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EYFP"
     CDS             5560..5589
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             5590..5616
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    5686..6274
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    6340..6388
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6590..7018
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7032..7361
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7428..8222
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8398..8519
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8568..8584)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8592..8608
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8616..8646)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8661..8682
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8970..9558)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9729..10589)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10590..10694)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagc tagccaccat gcagagcaag gtgctgctgg ccgtcgccct gtggctctgc
      781 gtggagaccc gggccgcctc tgtgggtttg cctagtgttt ctcttgatct gcccaggctc
      841 agcatacaaa aagacatact tacaattaag gctaatacaa ctcttcaaat tacttgcagg
      901 ggacagaggg acttggactg gctttggccc aataatcaga gtggcagtga gcaaagggtg
      961 gaggtgactg agtgcagcga tggcctcttc tgtaagacac tcacaattcc aaaagtgatc
     1021 ggaaatgaca ctggagccta caagtgcttc taccgggaaa ctgacttggc ctcggtcatt
     1081 tatgtctatg ttcaagatta cagatctcca tttattgctt ctgttagtga ccaacatgga
     1141 gtcgtgtaca ttactgagaa caaaaacaaa actgtggtga ttccatgtct cgggtccatt
     1201 tcaaatctca acgtgtcact ttgtgcaaga tacccagaaa agagatttgt tcctgatggt
     1261 aacagaattt cctgggacag caagaagggc tttactattc ccagctacat gatcagctat
     1321 gctggcatgg tcttctgtga agcaaaaatt aatgatgaaa gttaccagtc tattatgtac
     1381 atagttgtcg ttgtagggta taggatttat gatgtggttc tgagtccgtc tcatggaatt
     1441 gaactatctg ttggagaaaa gcttgtctta aattgtacag caagaactga actaaatgtg
     1501 gggattgact tcaactggga atacccttct tcgaagcatc agcataagaa acttgtaaac
     1561 cgagacctaa aaacccagtc tgggagtgag atgaagaaat ttttgagcac cttaactata
     1621 gatggtgtaa cccggagtga ccaaggattg tacacctgtg cagcatccag tgggctgatg
     1681 accaagaaga acagcacatt tgtcagggtc catgaaaaac cttttgttgc ttttggaagt
     1741 ggcatggaat ctctggtgga agccacggtg ggggagcgtg tcagaatccc tgcgaagtac
     1801 cttggttacc cacccccaga aataaaatgg tataaaaatg gaatacccct tgagtccaat
     1861 cacacaatta aagcggggca tgtactgacg attatggaag tgagtgaaag agacacagga
     1921 aattacactg tcatccttac caatcccatt tcaaaggaga agcagagcca tgtggtctct
     1981 ctggttgtgt atgtcccacc ccagattggt gagaaatctc taatctctcc tgtggattcc
     2041 taccagtacg gcaccactca aacgctgaca tgtacggtct atgccattcc tcccccgcat
     2101 cacatccact ggtattggca gttggaggaa gagtgcgcca acgagcccag ccaagctgtc
     2161 tcagtgacaa acccataccc ttgtgaagaa tggagaagtg tggaggactt ccagggagga
     2221 aataaaattg aagttaataa aaatcaattt gctctaattg aaggaaaaaa caaaactgta
     2281 agtacccttg ttatccaagc ggcaaatgtg tcagctttgt acaaatgtga agcggtcaac
     2341 aaagtcggga gaggagagag ggtgatctcc ttccacgtga ccaggggtcc tgaaattact
     2401 ttgcaacctg acatgcagcc cactgagcag gagagcgtgt ctttgtggtg cactgcagac
     2461 agatctacgt ttgagaacct cacatggtac aagcttggcc cacagcctct gccaatccat
     2521 gtgggagagt tgcccacacc tgtttgcaag aacttggata ctctttggaa attgaatgcc
     2581 accatgttct ctaatagcac aaatgacatt ttgatcatgg agcttaagaa tgcatccttg
     2641 caggaccaag gagactatgt ctgccttgct caagacagga agaccaagaa aagacattgc
     2701 gtggtcaggc agctcacagt cctagagcgt gtggcaccca cgatcacagg aaacctggag
     2761 aatcagacga caagtattgg ggaaagcatc gaagtctcat gcacggcatc tgggaatccc
     2821 cctccacaga tcatgtggtt taaagataat gagacccttg tagaagactc aggcattgta
     2881 ttgaaggatg ggaaccggaa cctcactatc cgcagagtga ggaaggagga cgaaggcctc
     2941 tacacctgcc aggcatgcag tgttcttggc tgtgcaaaag tggaggcatt tttcataata
     3001 gaaggtgccc aggaaaagac gaacttggaa atcattattc tagtaggcac ggcggtgatt
     3061 gccatgttct tctggctact tcttgtcatc atcctacgga ccgttaagcg ggccaatgga
     3121 ggggaactga agacaggcta cttgtccatc gtcatggatc cagatgaact cccattggat
     3181 gaacattgtg aacgactgcc ttatgatgcc agcaaatggg aattccccag agaccggctg
     3241 aagctaggta agcctcttgg ccgtggtgcc tttggccaag tgattgaagc agatgccttt
     3301 ggaattgaca agacagcaac ttgcaggaca gtagcagtca aaatgttgaa agaaggagca
     3361 acacacagtg agcatcgagc tctcatgtct gaactcaaga tcctcattca tattggtcac
     3421 catctcaatg tggtcaacct tctaggtgcc tgtaccaagc caggagggcc actcatggtg
     3481 attgtggaat tctgcaaatt tggaaacctg tccacttacc tgaggagcaa gagaaatgaa
     3541 tttgtcccct acaagaccaa aggggcacga ttccgtcaag ggaaagacta cgttggagca
     3601 atccctgtgg atctgaaacg gcgcttggac agcatcacca gtagccagag ctcagccagc
     3661 tctggatttg tggaggagaa gtccctcagt gatgtagaag aagaggaagc tcctgaagat
     3721 ctgtataagg acttcctgac cttggagcat ctcatctgtt acagcttcca agtggctaag
     3781 ggcatggagt tcttggcatc gcgaaagtgt atccacaggg acctggcggc acgaaatatc
     3841 ctcttatcgg agaagaacgt ggttaaaatc tgtgactttg gcttggcccg ggatatttat
     3901 aaagatccag attatgtcag aaaaggagat gctcgcctcc ctttgaaatg gatggcccca
     3961 gaaacaattt ttgacagagt gtacacaatc cagagtgacg tctggtcttt tggtgttttg
     4021 ctgtgggaaa tattttcctt aggtgcttct ccatatcctg gggtaaagat tgatgaagaa
     4081 ttttgtaggc gattgaaaga aggaactaga atgagggccc ctgattatac tacaccagaa
     4141 atgtaccaga ccatgctgga ctgctggcac ggggagccca gtcagagacc cacgttttca
     4201 gagttggtgg aacatttggg aaatctcttg caagctaatg ctcagcagga tggcaaagac
     4261 tacattgttc ttccgatatc agagactttg agcatggaag aggattctgg actctctctg
     4321 cctacctcac ctgtttcctg tatggaggag gaggaagtat gtgaccccaa attccattat
     4381 gacaacacag caggaatcag tcagtatctg cagaacagta agcgaaagag ccggcctgtg
     4441 agtgtaaaaa catttgaaga tatcccgtta gaagaaccag aagtaaaagt aatcccagat
     4501 gacaaccaga cggacagtgg tatggttctt gcctcagaag agctgaaaac tttggaagac
     4561 agaaccaaat tatctccatc ttttggtgga atggtgccca gcaaaagcag ggagtctgtg
     4621 gcatctgaag gctcaaacca gacaagcggc taccagtccg gatatcactc cgatgacaca
     4681 gacaccaccg tgtactccag tgaggaagca gaacttttaa agctgataga gattggagtg
     4741 caaaccggta gcacagccca gattctccag cctgactcgg ggaccacact gagctctcct
     4801 cctgttggta ccggtggagg cggatcagga ggcggtggat ctatggtgag caagggcgag
     4861 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     4921 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     4981 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc
     5041 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     5101 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5161 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5221 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5281 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     5341 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     5401 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     5461 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     5521 gccgccggga tcactctcgg catggacgag ctgtacaagg agcaaaagct catttctgaa
     5581 gaggacttgt acccatacga cgtaccagat tacgcttagc tcgagtctag agggcccctt
     5641 aagggttcga tccctaccgg ttagtaatga gtttgatatc tcgacaatca acctctggat
     5701 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5761 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5821 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5881 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5941 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6001 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6061 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     6121 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6181 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6241 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa acgggggagg ctaactgaaa
     6301 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     6361 aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     6421 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     6481 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     6541 ggccctgcca tagcagatct gcgcagctgg ggctctaggg ggtatcccca cgcgccctgt
     6601 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6661 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6721 tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg
     6781 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6841 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6901 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6961 gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     7021 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7081 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7141 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7201 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7261 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     7321 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     7381 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     7441 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     7501 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     7561 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     7621 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     7681 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     7741 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     7801 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     7861 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     7921 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     7981 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     8041 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     8101 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     8161 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     8221 gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     8281 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     8341 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     8401 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8461 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8521 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     8581 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8641 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8701 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8761 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8821 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8881 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8941 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9001 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9061 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9121 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     9181 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9241 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9301 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9361 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     9421 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9481 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9541 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9601 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9661 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9721 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9781 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9841 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9901 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9961 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10021 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10081 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10141 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10201 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10261 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10321 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10381 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10441 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10501 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10561 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10621 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10681 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
    10741 ctcccgatcc cctatggtcg actctcagta caatctgctc tgatgccgca tagttaagcc
    10801 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
    10861 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
//
