LOCUS       V019538                 8519 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019538
VERSION     V019538
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             683..3178
                     /label="KAT2B(NM_003884)"
                     /note="KAT2B(NM_003884)"
                     /gene="KAT2B"
     CDS             3185..3211
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3215..3241
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3245..3271
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3295..3883
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3949..3997
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4199..4627
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4641..4970
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5037..5831
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6007..6128
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6177..6193)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6201..6217
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6225..6255)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6270..6291
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6579..7167)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7338..8198)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8199..8303)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatgtccga ggctggcggg gccgggccgg gcggctgcgg
      721 ggcaggagcc ggggcagggg ccgggcccgg ggcgctgccc ccgcagcctg cggcgcttcc
      781 gcccgcgccc ccgcagggct ccccctgcgc cgctgccgcc gggggctcgg gcgcctgcgg
      841 tccggcgacg gcagtggctg cagcgggcac ggccgaagga ccgggaggcg gtggctcggc
      901 ccgaatcgcc gtgaagaaag cgcaactacg ctccgctccg cgggccaaga aactggagaa
      961 actcggagtg tactccgcct gcaaggccga ggagtcttgt aaatgtaatg gctggaaaaa
     1021 ccctaacccc tcacccactc cccccagagc cgacctgcag caaataattg tcagtctaac
     1081 agaatcctgt cggagttgta gccatgccct agctgctcat gtttcccacc tggagaatgt
     1141 gtcagaggaa gaaatgaaca gactcctggg aatagtattg gatgtggaat atctctttac
     1201 ctgtgtccac aaggaagaag atgcagatac caaacaagtt tatttctatc tatttaagct
     1261 cttgagaaag tctattttac aaagaggaaa acctgtggtt gaaggctctt tggaaaagaa
     1321 acccccattt gaaaaaccta gcattgaaca gggtgtgaat aactttgtgc agtacaaatt
     1381 tagtcacctg ccagcaaaag aaaggcaaac aatagttgag ttggcaaaaa tgttcctaaa
     1441 ccgcatcaac tattggcatc tggaggcacc atctcaacga agactgcgat ctcccaatga
     1501 tgatatttct ggatacaaag agaactacac aaggtggctg tgttactgca acgtgccaca
     1561 gttctgcgac agtctacctc ggtacgaaac cacacaggtg tttgggagaa cattgcttcg
     1621 ctcggtcttc actgttatga ggcgacaact cctggaacaa gcaagacagg aaaaagataa
     1681 actgcctctt gaaaaacgaa ctctaatcct cactcatttc ccaaaatttc tgtccatgct
     1741 agaagaagaa gtatatagtc aaaactctcc catctgggat caggattttc tctcagcctc
     1801 ttccagaacc agccagctag gcatccaaac agttatcaat ccacctcctg tggctgggac
     1861 aatttcatac aattcaacct catcttccct tgagcagcca aacgcaggga gcagcagtcc
     1921 tgcctgcaaa gcctcttctg gacttgaggc aaacccagga gaaaagagga aaatgactga
     1981 ttctcatgtt ctggaggagg ccaagaaacc ccgagttatg ggggatattc cgatggaatt
     2041 aatcaacgag gttatgtcta ccatcacgga ccctgcagca atgcttggac cagagaccaa
     2101 ttttctgtca gcacactcgg ccagggatga ggcggcaagg ttggaagagc gcaggggtgt
     2161 aattgaattt cacgtggttg gcaattccct caaccagaaa ccaaacaaga agatcctgat
     2221 gtggctggtt ggcctacaga acgttttctc ccaccagctg ccccgaatgc caaaagaata
     2281 catcacacgg ctcgtctttg acccgaaaca caaaaccctt gctttaatta aagatggccg
     2341 tgttattggt ggtatctgtt tccgtatgtt cccatctcaa ggattcacag agattgtctt
     2401 ctgtgctgta acctcaaatg agcaagtcaa gggctatgga acacacctga tgaatcattt
     2461 gaaagaatat cacataaagc atgacatcct gaacttcctc acatatgcag atgaatatgc
     2521 aattggatac tttaagaaac agggtttctc caaagaaatt aaaataccta aaaccaaata
     2581 tgttggctat atcaaggatt atgaaggagc cactttaatg ggatgtgagc taaatccacg
     2641 gatcccgtac acagaatttt ctgtcatcat taaaaagcag aaggagataa ttaaaaaact
     2701 gattgaaaga aaacaggcac aaattcgaaa agtttaccct ggactttcat gttttaaaga
     2761 tggagttcga cagattccta tagaaagcat tcctggaatt agagagacag gctggaaacc
     2821 gagtggaaaa gagaaaagta aagagcccag agaccctgac cagctttaca gcacgctcaa
     2881 gagcatcctc cagcaggtga agagccatca aagcgcttgg cccttcatgg aacctgtgaa
     2941 gagaacagaa gctccaggat attatgaagt tataaggttc cccatggatc tgaaaaccat
     3001 gagtgaacgc ctcaagaata ggtactacgt gtctaagaaa ttattcatgg cagacttaca
     3061 gcgagtcttt accaattgca aagagtacaa cccccctgag agtgaatact acaaatgtgc
     3121 caatatcctg gagaaattct tcttcagtaa aattaaggaa gctggattaa ttgacaagct
     3181 cgagtaccca tacgacgtac ctgattatgc aacttatcct tatgatgtac ctgattatgc
     3241 tagctatcct tatgatgtcc cagactacgc atagtgaggg cccgatatct cgacaatcaa
     3301 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3361 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3421 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3481 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3541 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3601 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3661 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     3721 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3781 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3841 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaaa cgggggaggc
     3901 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     3961 gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     4021 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     4081 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     4141 ggggcggcag gccctgccat agcagatctg cgcagctggg gctctagggg gtatccccac
     4201 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4261 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4321 ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt
     4381 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4441 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4501 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4561 gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4621 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     4681 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4741 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4801 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4861 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4921 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4981 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     5041 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5101 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5161 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     5221 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5281 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5341 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5401 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5461 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5521 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     5581 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5641 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5701 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5761 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5821 agttcttctg agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg
     5881 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5941 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     6001 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6061 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6121 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6181 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6241 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6301 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6361 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     6781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     7021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     8341 tcgggagatc tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat
     8401 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     8461 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttagg
//
