LOCUS V030987 5883 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030987 VERSION V030987 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..2030 /label="Jun(NM_010591)" /note="Jun(NM_010591)" /gene="Jun" CDS 2049..2078 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2097..2120 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2140..2156) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2167..2789 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2938..3526) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3855..3902 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4134..4928) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4963..5320) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5322..5426) /gene="bla" /label="AmpR promoter" rep_origin 5453..5881 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gactgcaaag atggaaacga ccttctacga cgatgccctc aacgcctcgt 1081 tcctccagtc cgagagcggt gcctacggct acagtaaccc taagatccta aaacagagca 1141 tgaccttgaa cctggccgac ccggtgggca gtctgaagcc gcacctccgc gccaagaact 1201 cggaccttct cacgtcgccc gacgtcgggc tgctcaagct ggcgtcgccg gagctggagc 1261 gcctgatcat ccagtccagc aatgggcaca tcaccactac accgaccccc acccagttct 1321 tgtgccccaa gaacgtgacc gacgagcagg agggcttcgc cgagggcttc gtgcgcgccc 1381 tggctgaact gcatagccag aacacgcttc ccagtgtcac ctccgcggca cagccggtca 1441 gcggggcggg catggtggct cccgcggtgg cctcagtagc aggcgctggc ggcggtggtg 1501 gctacagcgc cagcctgcac agtgagcctc cggtctacgc caacctcagc aacttcaacc 1561 cgggtgcgct gagcagcggc ggtggggcgc cctcctatgg cgcggccggg ctggcctttc 1621 cctcgcagcc gcagcagcag cagcagccgc ctcagccgcc gcaccacttg ccccaacaga 1681 tcccggtgca gcacccgcgg ctgcaagccc tgaaggaaga gccgcagacc gtgccggaga 1741 tgccgggaga gacgccgccc ctgtccccta tcgacatgga gtctcaggag cggatcaagg 1801 cagagaggaa gcgcatgagg aaccgcattg ccgcctccaa gtgccggaaa aggaagctgg 1861 agcggatcgc tcggctagag gaaaaagtga aaaccttgaa agcgcaaaac tccgagctgg 1921 catccacggc caacatgctc agggaacagg tggcacagct taagcagaaa gtcatgaacc 1981 acgttaacag tgggtgccaa ctcatgctaa cgcagcagtt gcaaacgttt acgcgtacgc 2041 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt 2101 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca 2161 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc 2221 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag 2281 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg 2341 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa 2401 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc 2461 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag 2521 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac 2581 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc 2641 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct 2701 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt 2761 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc 2821 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 2881 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 2941 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 3001 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 3061 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 3121 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 3181 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 3241 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 3301 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 3361 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 3421 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 3481 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 3541 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 3601 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 3661 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc 3721 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg 3781 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg 3841 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc 3901 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct 3961 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc 4021 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg 4081 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag 4141 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa 4201 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc 4261 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa 4321 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga 4381 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc 4441 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct 4501 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc 4561 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac 4621 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca 4681 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc 4741 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc 4801 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag 4861 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt 4921 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc 4981 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc 5041 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt 5101 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat 5161 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta 5221 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc 5281 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat 5341 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 5401 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc 5461 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 5521 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 5581 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 5641 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 5701 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 5761 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 5821 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 5881 ttt //