LOCUS       V030848                 8100 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030848
VERSION     V030848
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..4247
                     /label="Itga6(NM_008397)"
                     /note="Itga6(NM_008397)"
                     /gene="Itga6"
     CDS             4266..4295
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             4314..4337
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4357..4373)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4384..5006
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(5155..5743)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    6072..6119
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(6351..7145)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(7180..7537)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7539..7643)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7670..8098
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcggtcgcg ggccagttgt gcctgctcta cctgtccgcg gggcttctag
     1081 cccggctggg tacagccttc aacctggaca cccgcgagga caacgtgatc cggaaatcgg
     1141 gggatcccgg gagcctcttc ggcttctcgc tcgccatgca ctggcagttg cagccggagg
     1201 acaagcggct gttgcttgtg ggggcacctc gggcagaagc actcccgctg cagagggcga
     1261 acagaacagg gggcctgtac agctgtgaca tcacctcccg gggaccttgt acacggattg
     1321 aatttgataa tgacgctgat cctatgtcag aaagcaagga agaccagtgg atgggagtca
     1381 ctgttcagag ccaaggtcca gggggcaaag tggtgacgtg tgcacatcga tatgagaaac
     1441 ggcagcacgt caacacgaag caggagtcgc gggatatctt tggaagatgt tatgtcctaa
     1501 gtcagaatct cagaattgaa gatgatatgg acggaggaga ctggagtttc tgcgatggcc
     1561 ggttgagagg ccatgaaaag tttggctcct gtcagcaagg agtagcggct actttcacta
     1621 aggactttca ttacattgtt tttggagccc cagggactta caactggaaa gggatcgtcc
     1681 gtgtagaaca aaagaataac actttttttg acatgaacat ctttgaagat gggccctatg
     1741 aagttggtgg agagactgat catgatgaaa gtctcgtgcc cgttcctgct aacagttacc
     1801 taggcttttc actggactca gggaagggta ttgtttctaa agatgacatc acttttgtgt
     1861 ctggtgctcc aagagccaat cacagtgggg ctgtagtttt gctaaaaaga gacatgaagt
     1921 ccgcgcatct gctccctgag tatatatttg acggagaagg cctggcttcc tcgtttggct
     1981 atgatgtggc agtggtggac ctcaatgcag atgggtggca agacattgtt atcggagctc
     2041 cacagtattt tgatagggat ggtgaagtcg ggggtgcagt ttacgtctac attaaccagc
     2101 aaggcaaatg gagtaatgtg aagccgattc gtctaaatgg gaccaaagac tcgatgtttg
     2161 gaatctctgt gaaaaatata ggtgatatta accaagatgg ctatccagat attgctgttg
     2221 gagctcccta tgatgatctg gggaaggttt ttatctatca tggatccccg actggcataa
     2281 ttaccaagcc aacacaggtt ctcgagggga catcgcctta cttcggctat tcaatcgctg
     2341 ggaatatgga cctggatcgg aattcctacc ccgaccttgc tgtgggctcc ctctcagact
     2401 cggtcactat tttcagatcc cggccagtga ttaacattct aaaaaccatc acagtgactc
     2461 ctaacagaat tgacctccgc cagaagtcca tgtgtggctc acctagcggg atatgcctca
     2521 aggttaaagc ctgttttgaa tatactgcga aaccttccgg ttataaccct ccaatatcaa
     2581 ttttgggtat tctcgaagct gaaaaagaaa gaagaaagtc agggttgtca tcgagagttc
     2641 agtttcgaaa ccaaggttcc gagccaaagt atactcagga gctgaccctg aatcggcaga
     2701 agcagcgggc gtgcatggag gagaccctct ggctgcagga gaacatcaga gacaagctgc
     2761 gtcccatccc catcacggct tctgtggaga tccaggagcc cagctctcgc cggcgggtga
     2821 actcactccc cgaagttctt cccatcctga attcaaatga agccaaaacg gtccagacag
     2881 atgtccactt cttaaaggaa ggatgtggag acgacaatgt ctgtaacagc aaccttaagc
     2941 tagagtataa atttggtacc cgagaaggaa atcaagacaa attctcttac cttccaattc
     3001 aaaaaggcat cccagaatta gtcctaaaag atcagaaaga tatagctctg gaaataacgg
     3061 tgaccaacag cccttcggat ccaaggaatc cccggaaaga tggcgacgat gcccatgaag
     3121 ccaaactcat cgccacgttt ccagacactc tgacatattc cgcttacaga gaactgaggg
     3181 ctttccctga gaagcagctg agctgtgtgg ccaaccagaa tggctcccaa gccgactgtg
     3241 agctcggaaa tcctttcaag agaaattcca gtgttacttt ctatctgatt ttaagtacaa
     3301 ccgaggtcac ctttgacacc acagatctgg atattaatct gaagttggaa acaacaagca
     3361 atcaggataa tttggctcca attacagcga aggcaaaagt ggttattgaa ttgcttttat
     3421 cggtctccgg agtcgctaag ccttcgcagg tgtattttgg aggtacagtt gttggtgagc
     3481 aagctatgaa atctgaagat gaagtaggaa gtttaataga gtatgaattt agggtgatta
     3541 acttaggcaa gcctcttaaa aacctcggca cagcaacctt gaatatacag tggcccaagg
     3601 agattagcaa tggcaaatgg ttgctttatt tgatgaaagt tgaatccaaa ggtttggagc
     3661 agattgtttg tgagccacac aatgaaataa actacctgaa gctgaaggag tctcacaact
     3721 caagaaagaa acgggaactt cctgaaaaac agatagatga cagcaggaaa ttttctttat
     3781 ttcctgaaag aaaataccag actctcaact gcagcgtcaa cgtgaggtgt gtgaacatca
     3841 ggtgcccact gcgagggctg gacagcaagg cctctctcgt tcttcgttcc aggttgtgga
     3901 acagcacatt tctagaggaa tattccaaac tgaactactt ggacattctc ctgagggctt
     3961 ccatagatgt caccgctgct gctcagaata tcaagctccc tcacgcgggc actcaggttc
     4021 gagtgacggt gtttccctca aagactgtag ctcagtattc aggagtagct tggtggatca
     4081 tcctcctggc tgttcttgcc gggattctga tgctggctct attagtgttt ttactgtgga
     4141 agtgtggctt cttcaagaga aataagaaag atcattacga tgccacctat cacaaggctg
     4201 agatccatac tcagccgtct gataaagaga ggcttacttc cgatgcaacg cgtacgcggc
     4261 cgctcgagca gaaactcatc tcagaagagg atctggcagc aaatgatatc ctggattaca
     4321 aggatgacga cgataaggtt taaacggccg gccgcggtca tagctgtttc ctgaacagat
     4381 cccgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     4441 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     4501 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     4561 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     4621 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     4681 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     4741 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     4801 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     4861 ctgattttaa aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc
     4921 catgcccaac cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg
     4981 tccactcagt agatgcctgt tgaattgggt acgcggccag cggcgagcgg tatcagctca
     5041 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5101 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5161 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5221 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5281 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5341 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5401 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5461 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5521 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5581 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5641 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5701 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5761 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5821 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5881 ctaaagtata tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc
     5941 gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc
     6001 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac
     6061 cccgcgttta tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt
     6121 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg
     6181 gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg
     6241 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
     6301 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
     6361 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     6421 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     6481 gctatgtcct gatagcgatc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     6541 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
     6601 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     6661 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     6721 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     6781 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     6841 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     6901 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     6961 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     7021 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     7081 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     7141 atcatgcgaa acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc
     7201 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
     7261 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
     7321 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct
     7381 ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt
     7441 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa
     7501 ctgacacaca ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat
     7561 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7621 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     7681 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7741 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7801 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7861 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7921 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7981 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8041 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
//
