LOCUS       V029638                 5928 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029638
VERSION     V029638
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2075
                     /label="Isl1(NM_021459)"
                     /note="Isl1(NM_021459)"
                     /gene="Isl1"
     CDS             2094..2123
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2142..2165
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2185..2201)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2212..2834
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2983..3571)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3900..3947
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4179..4973)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5008..5365)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5367..5471)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5498..5926
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gggagacatg ggcgatccac caaaaaaaaa acgtctgatt tccctgtgtg
     1081 ttggttgcgg caatcaaatt cacgaccagt atattctgag ggtttctccg gatttggagt
     1141 ggcatgcagc atgtttgaaa tgtgcggagt gtaatcagta tttggacgaa agctgtacgt
     1201 gctttgttag ggatgggaaa acctactgta aaagagatta tatcaggttg tacgggatca
     1261 aatgcgccaa gtgcagcata ggcttcagca agaacgactt cgtgatgcgt gcccgctcta
     1321 aggtgtacca catcgagtgt ttccgctgtg tagcctgcag ccgacagctc atcccgggag
     1381 acgaattcgc cctgcgggag gatgggcttt tctgccgtgc agaccacgat gtggtggaga
     1441 gagccagcct gggagctgga gaccctctca gtcccttgca tccagcgcgg cctctgcaaa
     1501 tggcagccga acccatctcg gctaggcagc cagctctgcg gccgcacgtc cacaagcagc
     1561 cggagaagac cacccgagtg cggactgtgc tcaacgagaa gcagctgcac accttgcgga
     1621 cctgctatgc cgccaaccct cggccagatg cgctcatgaa ggagcaacta gtggagatga
     1681 cgggcctcag tcccagagtc atccgagtgt ggtttcaaaa caagcggtgc aaggacaaga
     1741 aacgcagcat catgatgaag cagctccagc agcagcaacc caacgacaaa actaatatcc
     1801 aggggatgac aggaactccc atggtggctg ctagtccgga gagacatgat ggtggtttac
     1861 aggctaaccc agtagaggtg caaagttacc agccgccctg gaaagtactg agtgacttcg
     1921 ccttgcaaag cgacatagat cagcctgctt ttcagcaact ggtcaatttt tcagaaggag
     1981 gaccaggctc taattctact ggcagtgaag tagcatcgat gtcctcgcag ctcccagata
     2041 cacccaacag catggtagcc agtcctattg aggcaacgcg tacgcggccg ctcgagcaga
     2101 aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag gatgacgacg
     2161 ataaggttta aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat
     2221 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     2281 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     2341 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     2401 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     2461 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     2521 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     2581 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     2641 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
     2701 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg
     2761 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag
     2821 atgcctgttg aattgggtac gcggccagcg gcgagcggta tcagctcact caaaggcggt
     2881 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2941 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3001 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3061 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3121 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3181 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3241 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3301 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3361 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3421 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3481 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3541 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3601 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3661 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3721 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc
     3781 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca
     3841 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
     3901 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
     3961 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
     4021 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     4081 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     4141 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     4201 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     4261 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     4321 tagcgatccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     4381 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     4441 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     4501 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     4561 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     4621 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     4681 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     4741 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca
     4801 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     4861 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     4921 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     4981 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc
     5041 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     5101 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     5161 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     5221 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     5281 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     5341 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat
     5401 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5461 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     5521 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5581 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5641 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5701 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5761 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5821 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5881 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaatttt
//
