LOCUS       V031060                 7770 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031060
VERSION     V031060
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3917
                     /label="Ireb2(NM_022655)"
                     /note="Ireb2(NM_022655)"
                     /gene="Ireb2"
     CDS             3936..3965
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3984..4007
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4027..4043)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4054..4676
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4825..5413)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5742..5789
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(6021..6815)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6850..7207)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7209..7313)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7340..7768
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggactcccca agtgcaggat acacctttga gtaccttatt gaaacattaa
     1081 atggcaattc acagaagaag ttcttcaatg tacctaaact tggcggcacc aagtatgata
     1141 ttctgcctta ctcaatacgg gtcctattgg aagctgctgt acgaaattgt gatggctttt
     1201 taatgaaaaa ggaagatgtt atgaacattt tggactggaa aaccaaacag agcaatgttg
     1261 aagtgccctt tttccctgcc cgtgttgttc ttcaagattt cactggaata ccggcaatgg
     1321 tggattttgc tgctatgagg gaggcagtga aaactcttgg aggtgatcct aagaaagtcc
     1381 accctgcctg tccgacagat ctcacagtgg accactcttt acagattgac ttcagtaaat
     1441 gtgcaataca gaatgcacca aatcctggag gtggtgacct acagaaagca ggaaagctct
     1501 ctccacttaa agtgcagtct aagaagcttc catgtcgagg ccagactacc tgccggggat
     1561 cgtgtgattc tggagagcta agccggaact caggaacatt ttcttcacag attgagaata
     1621 cacctgtctt gtgtcctttt catttgcaac cagtgcctga acctgagaca gtgttaaaaa
     1681 atcaagaagt agaatttggc agaaatcgag agaggcttca gtttttcaag tggagctcag
     1741 gagcttttaa gaatgtggca gtcatcccgc ctggaactgg aatggctcat caagtgaact
     1801 tagaatattt atcaagagta gtttttgaag aaacggacct gctcttccca gacagtgtag
     1861 ttggcacaga ttctcatata accatggtga acggattggg gattcttggg tggggagttg
     1921 gtggcattga gacagaggca gttatgcttg gcctgccagt tactcttact ttaccagagg
     1981 tggttggatg tgagctaact gggtcatcca atgcttttgt tacatccata gatattgtcc
     2041 ttggcattac aaagcacctc aggcaagtag gcgtggctgg aaagtttgtt gagttctttg
     2101 gaagtggagt ttcacaatta tctattgtcg atagaactac catagcaaac atgtgtccag
     2161 aatatggtgc tatcctcagc tttttccctg ttgacaatgt gacactacga catttagaac
     2221 atacaggttt tgacaaaacc aaacttgagt caatggaaaa ataccttaaa gctgtgaaat
     2281 tgtttcgaaa tgatgagaat tcttcagaac ctgaatattc tcaggtgata cagattaatc
     2341 tgaattcaat agttgcatct gtcagtggtc caaaaaggcc tcaggatagg gttgctgtaa
     2401 cagatatgaa aagtgatttt caggcttgct taaatgaaaa ggttggattt aaaggcttcc
     2461 aagttgcagc agaaaaacaa agtgacactg tctctgttcg ttatgatgga agtgagtata
     2521 agctgtccca tggatccgtg gtcattgctg cagttatcag ttgtaccaat aattgcaatc
     2581 catctgtgat gcttgctgca ggtcttctgg ctaaaaaggc tgttgaaatt ggtctacgag
     2641 ttaaacctta tatacgaaca agtttgtctc caggcagtgg gatggttaca cattacctca
     2701 gttcaagtgg agtgctaccc tatcttagca agctagggtt tgacatagtt ggctatggat
     2761 gttcaacatg tgtaggaaat acagcacctt tatcagaagc agttttgaat gcagtaaaac
     2821 agggtgattt ggttacctgt ggagttttat ctggaaacaa acatttcgaa ggccgtctct
     2881 gtgattgtgt ccgtgccaat tatctcgcct ctccgccctt agtggtggct tatgccatag
     2941 caggcacagt gaatatagac ttccagacag agcctttagg tactgactct acgggcaagg
     3001 aaatttacct gcatgacatt tggcctagtc gagaagaagt tcatcagatg gaggaagaac
     3061 atgttatatt gtcaatgttt aaaacactaa aagagaaagt agagatggga aataaacggt
     3121 ggaattcgtt agaagctcca gactccgtgc tgtttccatg ggatgtgaag tctacttaca
     3181 tcagatgccc ttcatttttt gataagctta ccaaagagcc agctgcatcc cagcctattg
     3241 agaatgccca tgtcctgttg tatttgggag actctgtcac tacagatcat atatcacccg
     3301 ctggaagtat tgctaggagt agtgctgctg ctaagtattt gacaaacaga ggccttactc
     3361 cccgagagtt caactcttat ggagctcgaa gaggtaatga tgctgtgatg acaagaggca
     3421 catttgcaaa catcaagctt tttaataagt ttattgggaa gccagctccc aaaacaattc
     3481 attttccatc gggacaaacg ctcgatgtat ttgaagctgc agaactgtac cagaaagaag
     3541 gtatcccact gataatttta gcaggaaaaa aatatggttc aggaaattca agagattggg
     3601 ctgccaaggg accctatttg ctgggtgtaa aagctgtttt ggctgagagc tatgaaaaaa
     3661 tacacaaaga tcatttgatt ggaattggta tagcaccact tgaattcctc ccaggagaaa
     3721 atgcagattc cttgggcctc tctggcagag aagtattttc tttatcattt cctgaagaac
     3781 tctttcctgg aattacatta aatataaaga caagcactgg aaaagagttc agcgtgattg
     3841 catcatttgc aaatgacgtg gagataactt tgtacaaaca tggaggctta ttaaactttg
     3901 tggctcgaaa attcttaacg cgtacgcggc cgctcgagca gaaactcatc tcagaagagg
     3961 atctggcagc aaatgatatc ctggattaca aggatgacga cgataaggtt taaacggccg
     4021 gccgcggtca tagctgtttc ctgaacagat cccgggtggc atccctgtga cccctcccca
     4081 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     4141 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     4201 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     4261 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     4321 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     4381 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     4441 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     4501 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag
     4561 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc
     4621 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt
     4681 acgcggccag cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4741 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4801 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4861 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4921 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4981 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5041 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5101 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5161 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5221 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5281 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5341 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5401 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5461 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5521 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat
     5581 ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     5641 gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     5701 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     5761 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
     5821 cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc
     5881 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     5941 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     6001 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     6061 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     6121 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcgatc cgccacaccc
     6181 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     6241 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     6301 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     6361 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     6421 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     6481 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     6541 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     6601 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     6661 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     6721 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     6781 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     6841 tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     6901 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     6961 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     7021 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     7081 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     7141 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc
     7201 gcctcaggac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7261 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7321 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7381 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7441 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7501 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7561 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7621 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7681 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7741 ctgatttaac aaaaatttaa cgcgaatttt
//
