LOCUS       V031205                 6582 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031205
VERSION     V031205
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2729
                     /label="Il1rl1(NM_001420349)"
                     /note="Il1rl1(NM_001420349)"
                     /gene="Il1rl1"
     CDS             2748..2777
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2796..2819
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2839..2855)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2866..3488
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3637..4225)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4554..4601
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4833..5627)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5662..6019)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6021..6125)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6152..6580
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gattgacaga cagagaatgg gactttgggc tttggcaatt ctgacacttc
     1081 ccatgtattt gacagttacg gagggcagta aatcgtcctg gggtctggaa aatgaggctt
     1141 taattgtgag atgcccccaa agaggacgct cgacttatcc tgtggaatgg tattactcag
     1201 atacaaatga aagtattcct actcaaaaaa gaaatcggat ctttgtctca agagatcgtc
     1261 tgaagtttct accagccaga gtggaagact ctgggattta tgcttgtgtt atcagaagcc
     1321 ccaacttgaa taagactgga tacttgaatg tcaccataca taaaaagccg ccaagctgca
     1381 atatccctga ttatttgatg tactcgacag tacgtggatc agataaaaat ttcaagataa
     1441 cgtgtccaac aattgacctg tataattgga cagcacctgt tcagtggttt aagaactgca
     1501 aagctctcca agagccaagg ttcagggcac acaggtccta cttgttcatt gacaacgtga
     1561 ctcatgatga tgaaggtgac tacacttgtc aattcacaca cgcggagaat ggaaccaact
     1621 acatcgtgac ggccaccaga tcattcacag ttgaagaaaa aggcttttct atgtttccag
     1681 taattacaaa tcctccatac aaccacacaa tggaagtgga aataggaaaa ccagcaagta
     1741 ttgcctgttc agcttgcttt ggcaaaggct ctcacttctt ggctgatgtc ctgtggcaga
     1801 ttaacaaaac agtagttgga aattttggtg aagcaagaat tcaagaagag gaaggtcgaa
     1861 atgaaagttc cagcaatgac atggattgtt taacctcagt gttaaggata actggtgtga
     1921 cagaaaagga cctgtccctg gaatatgact gtctggccct gaaccttcat ggcatgataa
     1981 ggcacaccat aaggctgaga aggaaacaac caattgatca ccgaagcatc tactacatag
     2041 ttgctggatg tagtttattg ctaatgttta tcaatgtctt ggtgatagtc ttaaaagtgt
     2101 tctggattga ggttgctctg ttctggagag atatagtgac accttacaaa acccggaacg
     2161 atggcaagct ctacgatgcg tacatcattt accctcgggt cttccggggc agcgcggcgg
     2221 gaacccactc tgtggagtac tttgttcacc acactctgcc cgacgttctt gaaaataaat
     2281 gtggctacaa attgtgcatt tatgggagag acctgttacc tgggcaagat gcagccaccg
     2341 tggtggaaag cagtatccag aatagcagaa gacaggtgtt tgttctggcc cctcacatga
     2401 tgcacagcaa ggaatttgcc tacgagcagg agattgctct gcacagcgcc ctcatccaga
     2461 acaactccaa ggtgattctt attgaaatgg agcctctggg tgaggcaagc cgactacagg
     2521 ttggggacct gcaagattct ctccagcatc ttgtgaaaat tcaggggacc atcaagtgga
     2581 gggaagatca tgtggccgac aagcagtctc taagttccaa attctggaag catgtgaggt
     2641 accaaatgcc agtgccagaa agagcctcca agacggcatc tgttgcggct ccgttgagtg
     2701 gcaaggcatg cttagacctg aaacacttta cgcgtacgcg gccgctcgag cagaaactca
     2761 tctcagaaga ggatctggca gcaaatgata tcctggatta caaggatgac gacgataagg
     2821 tttaaacggc cggccgcggt catagctgtt tcctgaacag atcccgggtg gcatccctgt
     2881 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     2941 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     3001 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     3061 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     3121 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     3181 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     3241 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     3301 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta
     3361 taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga
     3421 catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct
     3481 gttgaattgg gtacgcggcc agcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3541 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3601 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3661 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3721 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3781 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3841 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3901 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3961 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4021 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4081 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4141 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4201 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4261 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4321 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4381 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     4441 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     4501 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     4561 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     4621 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     4681 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     4741 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     4801 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     4861 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     4921 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcga
     4981 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     5041 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     5101 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     5161 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     5221 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     5281 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     5341 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     5401 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     5461 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     5521 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     5581 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     5641 catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     5701 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     5761 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     5821 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     5881 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     5941 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     6001 ctggttcttt ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt
     6061 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     6121 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     6181 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6241 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6301 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6361 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6421 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6481 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6541 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt tt
//
