LOCUS       V030696                 5688 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030696
VERSION     V030696
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1835
                     /label="Il1b(NM_008361)"
                     /note="Il1b(NM_008361)"
                     /gene="Il1b"
     CDS             1854..1883
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1902..1925
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1945..1961)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1972..2594
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2743..3331)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3660..3707
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3939..4733)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4768..5125)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5127..5231)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5258..5686
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcaactgtt cctgaactca actgtgaaat gccacctttt gacagtgatg
     1081 agaatgacct gttctttgaa gttgacggac cccaaaagat gaagggctgc ttccaaacct
     1141 ttgacctggg ctgtccagat gagagcatcc agcttcaaat ctcacagcag cacatcaaca
     1201 agagcttcag gcaggcagta tcactcattg tggctgtgga gaagctgtgg cagctacctg
     1261 tgtctttccc gtggaccttc caggatgagg acatgagcac cttcttttcc ttcatctttg
     1321 aagaagagcc catcctctgt gactcatggg atgatgatga taacctgctg gtgtgtgacg
     1381 ttcccattag acagctgcac tacaggctcc gagatgaaca acaaaaaagc ctcgtgctgt
     1441 cggacccata tgagctgaaa gctctccacc tcaatggaca gaatatcaac caacaagtga
     1501 tattctccat gagctttgta caaggagaac caagcaacga caaaatacct gtggccttgg
     1561 gcctcaaagg aaagaatcta tacctgtcct gtgtaatgaa agacggcaca cccaccctgc
     1621 agctggagag tgtggatccc aagcaatacc caaagaagaa gatggaaaag cggtttgtct
     1681 tcaacaagat agaagtcaag agcaaagtgg agtttgagtc tgcagagttc cccaactggt
     1741 acatcagcac ctcacaagca gagcacaagc ctgtcttcct gggaaacaac agtggtcagg
     1801 acataattga cttcaccatg gaatctgtgt cttccacgcg tacgcggccg ctcgagcaga
     1861 aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag gatgacgacg
     1921 ataaggttta aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat
     1981 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     2041 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     2101 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     2161 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     2221 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     2281 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     2341 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     2401 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
     2461 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg
     2521 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag
     2581 atgcctgttg aattgggtac gcggccagcg gcgagcggta tcagctcact caaaggcggt
     2641 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2701 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2761 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2821 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2881 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2941 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3001 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3061 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3121 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3181 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3241 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3301 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3361 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3421 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3481 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc
     3541 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca
     3601 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
     3661 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
     3721 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
     3781 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     3841 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     3901 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     3961 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     4021 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     4081 tagcgatccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     4141 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     4201 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     4261 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     4321 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     4381 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     4441 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     4501 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca
     4561 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     4621 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     4681 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     4741 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc
     4801 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     4861 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     4921 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     4981 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     5041 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     5101 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat
     5161 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5221 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     5281 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5341 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5401 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5461 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5521 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5581 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5641 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaatttt
//
