LOCUS       V030833                 5886 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030833
VERSION     V030833
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2033
                     /label="Il12b(NM_001303244)"
                     /note="Il12b(NM_001303244)"
                     /gene="Il12b"
     CDS             2052..2081
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2100..2123
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2143..2159)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2170..2792
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2941..3529)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3858..3905
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4137..4931)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4966..5323)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5325..5429)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5456..5884
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gtgtcctcag aagctaacca tctcctggtt tgccatcgtt ttgctggtgt
     1081 ctccactcat ggccatgtgg gagctggaga aagacgttta tgttgtagag gtggactgga
     1141 ctcccgatgc ccctggagaa acagtgaacc tcacctgtga cacgcctgaa gaagatgaca
     1201 tcacctggac ctcagaccag agacatggag tcataggctc tggaaagacc ctgaccatca
     1261 ctgtcaaaga gtttctagat gctggccagt acacctgcca caaaggaggc gagactctga
     1321 gccactcaca tctgctgctc cacaagaagg aaaatggaat ttggtccact gaaattttaa
     1381 aaaatttcaa aaacaagact ttcctgaagt gtgaagcacc aaattactcc ggacggttca
     1441 cgtgctcatg gctggtgcaa agaaacatgg acttgaagtt caacatcaag agcagtagca
     1501 gttcccctga ctctcgggca gtgacatgtg gaatggcgtc tctgtctgca gagaaggtca
     1561 cactggacca aagggactat gagaagtatt cagtgtcctg ccaggaggat gtcacctgcc
     1621 caactgccga ggagaccctg cccattgaac tggcgttgga agcacggcag cagaataaat
     1681 atgagaacta cagcaccagc ttcttcatca gggacatcat caaaccagac ccgcccaaga
     1741 acttgcagat gaagcctttg aagaactcac aggtggaggt cagctgggag taccctgact
     1801 cctggagcac tccccattcc tacttctccc tcaagttctt tgttcgaatc cagcgcaaga
     1861 aagaaaagat gaaggagaca gaggaggggt gtaaccagaa aggtgcgttc ctcgtagaga
     1921 agacatctac cgaagtccaa tgcaaaggcg ggaatgtctg cgtgcaagct caggatcgct
     1981 attacaattc ctcatgcagc aagtgggcat gtgttccctg cagggtccga tccacgcgta
     2041 cgcggccgct cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg
     2101 attacaagga tgacgacgat aaggtttaaa cggccggccg cggtcatagc tgtttcctga
     2161 acagatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     2221 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     2281 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     2341 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     2401 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     2461 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     2521 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     2581 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     2641 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta
     2701 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc
     2761 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc
     2821 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2881 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2941 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3001 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3061 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3121 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3181 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3241 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3301 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3361 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3421 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3481 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3541 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3601 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3661 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt
     3721 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac
     3781 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga
     3841 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat
     3901 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc
     3961 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     4021 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     4081 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     4141 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     4201 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     4261 gccaacgcta tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca
     4321 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     4381 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc
     4441 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     4501 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     4561 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     4621 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     4681 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     4741 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     4801 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     4861 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     4921 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag
     4981 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     5041 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     5101 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     5161 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     5221 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     5281 ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa
     5341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     5461 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5521 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5581 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5641 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5701 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5761 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5821 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5881 aatttt
//
