LOCUS       V031016                 7860 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031016
VERSION     V031016
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..4007
                     /label="Ighmbp2(NM_009212)"
                     /note="Ighmbp2(NM_009212)"
                     /gene="Ighmbp2"
     CDS             4026..4055
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             4074..4097
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4117..4133)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4144..4766
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4915..5503)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5832..5879
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(6111..6905)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6940..7297)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7299..7403)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7430..7858
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcctcgtcc accgtggaga gtttcgtggc ccagcagcta cagctgctgg
     1081 agctggagcg ggacgccgag gtggaggagc gcaggtcctg gcaggaacac agttctctga
     1141 gagagcttca gagccgaggg gtgtgcttgc tgaagcttca ggtatccagc cagcgcacag
     1201 ggctgtatgg gcagcggctg gtcacctttg agcccaggaa gtttggacct gcagtggtgc
     1261 ttcctagcaa cagcttcacc tctggtgata ttgtgggtct gtatgatact aatgaaaaca
     1321 gtcaactggc cactggggtc ctgacccgaa tcacccaaaa atcagtcaca gtggcttttg
     1381 atgagtccca tgatctccag ctgaacctgg acagagagaa tacctacaga ctgctgaagc
     1441 ttgccaacga tgtcacctac aaacgcctga aaaaagcact gatgacactg aagaagtacc
     1501 actctgggcc agcatcctcc ctcatagaca tcctgttggg cagctccacc ccaagtcctg
     1561 ccatggaaat acccccactc tctttctaca acacaactct ggacctttcc cagaaagaag
     1621 ctgtgtcctt tgcactggcc cagaaagaac ttgccatcat ccatgggcct cctggcactg
     1681 ggaaaaccac aactgtggtg gaaataatcc ttcaagctgt gaagcaaggc ttaaaggtcc
     1741 tgtgctgtgc tccctccaac atcgctgtgg acaacctggt ggagcgtctg gctctgtgca
     1801 agaagcggat tctgcgcctg ggtcaccccg cccgcctcct ggagtccgtt cagcaccact
     1861 cactggatgc agtgctagca cgcagtgaca acgcccagat tgttgctgac atcagaaggg
     1921 acatagacca ggtctttggc aagaacaaaa agacgcagga taagagagag aaaggtaatt
     1981 ttcgaagtga aattaagctg ctaaggaagg aactgaagga gagggaagaa gcagccatag
     2041 ttcagagcct cactgcggca gatgtggttc tagccaccaa cacaggtgca tcgtctgatg
     2101 gccctctgaa gctgctgcct gaggactact ttgatgtggt ggtggtggac gaatgtgccc
     2161 aggccctaga agccagctgc tggatccccc tgctgaaggc cccaaagtgc atcctagctg
     2221 gggaccacag acagctgcca cccaccaccg tctctcacag ggcggcactg gctgggctgt
     2281 cccgcagcct gatggagcgt ctggcagaga agcatggtgc tggtgtggtg aggatgctga
     2341 cggtacagta ccgaatgcac caggccatca tgtgctgggc ctcggaggcc atgtaccacg
     2401 ggcagcttac ttcccatccc tctgtggcag gacacctcct gaaggacctc ccaggcgtga
     2461 ctgacacaga ggagacacgt gtcccgctgc tgctcataga caccgctggc tgtgggcttc
     2521 tggagctgga ggaggaggac agccagtcca agggaaatcc gggcgaagtt cgcctcgtca
     2581 ctttgcacat ccaggctctg gtggatgctg gggtccaggc aggtgacatt gccgtcatcg
     2641 caccctacaa ccttcaggtg gatctgctca gacagagcct ctccaacaag caccctgagc
     2701 tggagattaa gtctgttgat ggctttcaag gccgagagaa agaggctgtg ctcctgacct
     2761 ttgtcaggtc caataggaaa ggtgaagttg gttttctggc tgaggacagg cggattaatg
     2821 ttgctgttac ccgtgctcgg cggcacgtgg cagtcatctg tgattcccac actgtcaaca
     2881 accatgcttt tttgaagacc ttggtggatt atttcacaga gcatggggag gtacgcacag
     2941 cctttgaata cctggatgac atcgtccctg agaactacac ccatgagggt tcccagggcc
     3001 acagccgtgt ccccaaaccc aagtgcccca gcacctccat caggaagcct gccagtgatc
     3061 aggagagtgg ccaggagacc agagcagccc ctagacatgg ccgcaggaag ccaagcgaga
     3121 agcccccggg ctctcacgtc cagtcccagc acagctccag tgcaaatggc tctgacagaa
     3181 ctggaggccc agaccggaca gagcacttta gggctacgat tgaggagttt gtggctagca
     3241 aggagtccca gttggagttt cccacatccc tgagttccca tgacaggctg cgagtccacc
     3301 agttagctga ggagttcggg ctgaggcacg acagcaccgg ggaggggaag gctcggcaca
     3361 tcacagtgag caggaggagc cctgccagct ctggcagtgt agccccacag ccttcctcac
     3421 cgcccagccc tgcacaggct gagcctgagc ctcgggcaga ggagcccgtc accgtcgtgc
     3481 aggcacattg ccctgtacag ctggatctga aggcactgca tctggagagg ctgcagcggc
     3541 agcaaagctc ccaggctcag acagccaagg gtcagccagg tggggactcg aggccacaga
     3601 aggcttcaca gaagaaaaag aaaaaagaac caaaaggtcc agttatggct ctgccctgtg
     3661 aggaggactt cgacgccctg gtgtctgctg tggtgaaggc tgacaacacc tgtagcttct
     3721 ccaagtgctc ggccagcacc accactctgg gccagttctg catgcactgt agccaccgct
     3781 actgcctcag ccaccatctg cctgaaatcc acggctgtgg agaaaaggct cgtgcccatg
     3841 cccgccagag gattagccgg gaaggagtgc tgtatgcagg cagtgggacc aaggacaggg
     3901 ccctggaccc ggccaagagg gcccagctgc agaggaggct ggacaagaag ctgggcgagc
     3961 tcagcagcca gaggacaagc aggaagaagg agaaggagag ggggacaacg cgtacgcggc
     4021 cgctcgagca gaaactcatc tcagaagagg atctggcagc aaatgatatc ctggattaca
     4081 aggatgacga cgataaggtt taaacggccg gccgcggtca tagctgtttc ctgaacagat
     4141 cccgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     4201 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     4261 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     4321 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     4381 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     4441 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     4501 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     4561 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     4621 ctgattttaa aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc
     4681 catgcccaac cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg
     4741 tccactcagt agatgcctgt tgaattgggt acgcggccag cggcgagcgg tatcagctca
     4801 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4861 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4921 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4981 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5041 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5101 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5161 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5221 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5281 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5341 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5401 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5461 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5521 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5581 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5641 ctaaagtata tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc
     5701 gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc
     5761 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac
     5821 cccgcgttta tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt
     5881 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg
     5941 gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg
     6001 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
     6061 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
     6121 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     6181 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     6241 gctatgtcct gatagcgatc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     6301 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
     6361 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     6421 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     6481 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     6541 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     6601 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     6661 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     6721 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     6781 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     6841 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     6901 atcatgcgaa acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc
     6961 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
     7021 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
     7081 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct
     7141 ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt
     7201 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa
     7261 ctgacacaca ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat
     7321 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7381 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     7441 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7501 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7561 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7621 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7681 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7741 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7801 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
//
