LOCUS V030652 6521 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030652 VERSION V030652 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 683..1180 /label="IMMP1L(NM_144981)" /note="IMMP1L(NM_144981)" /gene="IMMP1L" CDS 1187..1213 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1217..1243 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1247..1273 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 1297..1885 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 1951..1999 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2201..2629 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2643..2972 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3039..3833 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4009..4130 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4179..4195) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4203..4219 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4227..4257) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4272..4293 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4581..5169) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5340..6200) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6201..6305) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccgcca ccatgcttcg tggtgttctg gggaaaacct ttcgacttgt 721 tggctatact attcaatatg gctgtatagc tcattgtgct tttgaatacg ttggtggtgt 781 tgtcatgtgt tctggaccat caatggagcc tacaattcaa aattcagata ttgtctttgc 841 agaaaatctt agtcgacatt tttatggtat ccaaagaggt gacattgtga ttgcaaaaag 901 cccaagtgat ccaaaatcaa atatttgtaa aagagtaatt ggtttggaag gagacaaaat 961 cctcaccact agtccatcag atttctttaa aagccatagt tatgtgccaa tgggtcatgt 1021 ttggttagaa ggtgacaatc tacagaattc tacagattcc aggtgctatg gacctattcc 1081 atatggacta ataagaggac gaatcttctt taagatttgg cctctgagtg attttggatt 1141 tttacgtgcc agccctaatg gccacagatt ttctgatgat ctcgagtacc catacgacgt 1201 acctgattat gcaacttatc cttatgatgt acctgattat gctagctatc cttatgatgt 1261 cccagactac gcatagtgag ggcccgatat ctcgacaatc aacctctgga ttacaaaatt 1321 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 1381 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 1441 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 1501 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 1561 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 1621 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 1681 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg 1741 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 1801 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 1861 atctcccttt gggccgcctc cccgcctgga aacgggggag gctaactgaa acacggaagg 1921 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg 1981 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat 2041 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac 2101 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc 2161 atagcagatc tgcgcagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 2221 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 2281 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 2341 caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac 2401 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 2461 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 2521 acaacactca accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg 2581 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 2641 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 2701 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 2761 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 2821 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 2881 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 2941 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 3001 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 3061 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 3121 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 3181 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 3241 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 3301 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 3361 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 3421 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 3481 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 3541 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 3601 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 3661 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 3721 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 3781 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 3841 tctggggttc gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 3901 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 3961 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 4021 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 4081 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 4141 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 4201 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 4261 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 4321 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 4381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 4441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 4501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 4561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 4621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 4681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 4741 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 4801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 4861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 4921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 4981 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 5041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 5101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 5161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 5221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 5281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 5341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 5401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 5461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 5521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 5581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 5641 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 5701 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 5761 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 5821 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 5881 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 5941 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 6001 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 6061 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 6121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 6181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 6241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 6301 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc 6361 ccctatggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc 6421 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa 6481 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag g //