LOCUS       V029943                 8401 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029943
VERSION     V029943
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3060
                     /label="IFI16(NM_001364867)"
                     /note="IFI16(NM_001364867)"
                     /gene="IFI16"
     CDS             3067..3093
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3097..3123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3127..3153
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3177..3765
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3831..3879
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4081..4509
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4523..4852
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4919..5713
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5889..6010
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6059..6075)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6083..6099
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6107..6137)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6152..6173
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6461..7049)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7220..8080)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8081..8185)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggg aaaaaaatac
      721 aagaacattg ttctactaaa aggattagag gtcatcaatg attatcattt tagaatggtt
      781 aagtccttac tgagcaacga tttaaaactt aatttaaaaa tgagagaaga gtatgacaaa
      841 attcagattg ctgacttgat ggaagaaaag ttccgaggtg atgctggttt gggcaaacta
      901 ataaaaattt tcgaagatat accaacgctt gaagacctgg ctgaaactct taaaaaagaa
      961 aagttaaaag taaaaggacc agccctatca agaaagagga agaaggaagt ggatgctact
     1021 tcacctgcac cctccacaag cagcactgtc aaaactgaag gagcagaggc aactcctgga
     1081 gctcagaaaa gaaaaaaatc aaccaaagaa aaggctggac ccaaagggag taaggtgtcc
     1141 gaggaacaga ctcagcctcc ctctcctgca ggagccggca tgtccacagc catgggccgt
     1201 tccccatctc ccaagacctc attgtcagct ccacccaaca gttcttcaac tgagaacccg
     1261 aaaacagtgg ccaaatgtca ggtaactccc agaagaaatg ttctccaaaa acgcccagtg
     1321 atagtgaagg tactgagtac aacaaagcca tttgaatatg agaccccaga aatggagaaa
     1381 aaaataatgt ttcatgctac agtggctaca cagacacagt tcttccatgt gaaggtttta
     1441 aacaccagct tgaaggagaa attcaatgga aagaaaatca tcatcatatc agattatttg
     1501 gaatatgata gtctcctaga ggtcaatgaa gaatctactg tatctgaagc tggtcctaac
     1561 caaacgtttg aggttccaaa taaaatcatc aacagagcaa aggaaactct gaagattgat
     1621 attcttcaca aacaagcttc aggaaatatt gtatatgggg tatttatgct acataagaaa
     1681 acagtaaatc agaagaccac aatctacgaa attcaggatg atagaggaaa aatggatgta
     1741 gtggggacag gacaatgtca caatatcccc tgtgaagaag gagataagct ccaacttttc
     1801 tgctttcgac ttagaaaaaa gaaccagatg tcaaaactga tttcagaaat gcatagtttt
     1861 atccagataa agaaaaaaac aaacccgaga aacaatgacc ccaagagcat gaagctaccc
     1921 caggaacagc gtcagcttcc atatccttca gaggccagca caaccttccc tgagagccat
     1981 cttcggactc ctcagatgcc accaacaact ccatccagca gtttcttcac caagaaaagt
     2041 gaagacacaa tctccaaaat gaatgacttc atgaggatgc agatactgaa ggaagggagt
     2101 cattttccag gaccgttcat gaccagcata ggcccagctg agagccatcc ccacactcct
     2161 cagatgcctc catcaacacc aagcagcagt ttcttaacca cgaaaagtga agacacaatc
     2221 tccaaaatga atgacttcat gaggatgcag atactgaagg aagggagtca ttttccagga
     2281 ccgttcatga ccagcatagg cccagctgag agccatcccc acactcctca gatgcctcca
     2341 tcaacaccaa gcagcagttt cttaaccacg ttgaaaccaa gactgaagac tgaacctgaa
     2401 gaagtttcca tagaagacag tgcccagagt gacctcaaag aagtgatggt gctgaacgca
     2461 acagaatcat ttgtatatga gcccaaagag cagaagaaaa tgtttcatgc cacagtggca
     2521 actgagaatg aagtcttccg agtgaaggtt tttaatattg acctaaagga gaagttcacc
     2581 ccaaagaaga tcattgccat agcaaattat gtttgccgca atgggttcct ggaggtatat
     2641 cctttcacac ttgtggctga tgtgaatgct gaccgaaaca tggagatccc aaaaggattg
     2701 attagaagtg ccagcgtaac tcctaaaatc aatcagcttt gctcacaaac taaaggaagt
     2761 tttgtgaatg gggtgtttga ggtacataag aaaaatgtaa ggggtgaatt cacttattat
     2821 gaaatacaag ataatacagg gaagatggaa gtggtggtgc atggacgact gaccacaatc
     2881 aactgtgagg aaggagataa actgaaactc acctgctttg aattggcacc gaaaagtggg
     2941 aataccgggg agttgagatc tgtaattcat agtcacatca aggtcatcaa gaccaggaaa
     3001 aacaagaaag acatactcaa tcctgattca agtatggaaa cttcaccaga ctttttcttc
     3061 ctcgagtacc catacgacgt acctgattat gcaacttatc cttatgatgt acctgattat
     3121 gctagctatc cttatgatgt cccagactac gcatagtgag ggcccgatat ctcgacaatc
     3181 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     3241 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3301 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     3361 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     3421 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     3481 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     3541 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     3601 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     3661 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     3721 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga aacgggggag
     3781 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     3841 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     3901 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     3961 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     4021 tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc
     4081 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4141 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4201 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta
     4261 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4321 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4381 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4441 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4501 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4561 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4621 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4681 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4741 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     4801 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     4861 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     4921 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     4981 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     5041 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     5101 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     5161 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     5221 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     5281 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     5341 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     5401 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     5461 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     5521 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     5581 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     5641 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     5701 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc
     5761 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5821 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     5881 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5941 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6001 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6061 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6121 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6181 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6241 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6301 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6361 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6421 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6481 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6541 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6601 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     6661 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6721 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6781 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6841 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6901 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6961 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7021 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7081 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7141 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7201 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7261 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7321 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7381 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7441 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7501 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7561 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7621 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7681 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7741 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7801 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7861 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7921 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7981 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8041 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8101 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8161 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     8221 gatcgggaga tctcccgatc ccctatggtc gactctcagt acaatctgct ctgatgccgc
     8281 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     8341 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     8401 g
//
