LOCUS       V030898                 6258 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030898
VERSION     V030898
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2405
                     /label="Htr2c(NM_008312)"
                     /note="Htr2c(NM_008312)"
                     /gene="Htr2c"
     CDS             2424..2453
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2472..2495
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2515..2531)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2542..3164
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3313..3901)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4230..4277
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4509..5303)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5338..5695)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5697..5801)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5828..6256
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggtgaacctg ggcactgcgg tgcgctcact ccttgtgcac ctaattggcc
     1081 tattggtttg gcagttcgat atttccataa gtccagtagc agctatagta actgacactt
     1141 ttaattcctc cgatggtgga cgcttgtttc aattcccgga cggggtacaa aactggccag
     1201 cactttcaat agtcgtgatt ataatcatga caataggggg caacattctc gttatcatgg
     1261 cagtaagcat ggagaagaaa ctgcacaatg ctaccaatta tttcttaatg tccctagcca
     1321 ttgctgatat gctggtggga ctacttgtca tgcccctgtc tctgcttgca attctttatg
     1381 attatgtctg gcctttacct agatatttgt gccccgtctg gatttcacta gatgtgctat
     1441 tttcaactgc gtccatcatg cacctctgcg ccatatcgct ggaccggtat gtagcaatac
     1501 gtaatcctat tgagcatagc cggttcaatt cgcggactaa ggccatcatg aagattgcca
     1561 tcgtttgggc aatatcaata ggagtttcag ttcctatccc tgtgattgga ctgagggacg
     1621 aaagcaaagt gttcgtgaat aatactacct gcgtgctcaa tgacccgaac ttcgttctca
     1681 tcgggtcctt cgtggcattc ttcatcccgt tgacaattat ggtgatcacc tacttcttaa
     1741 cgatctacgt cctacgccgt caaaccctga tgttacttcg aggtcacacc gaggaggaac
     1801 tgcgtaatat cagcctgaac tttctaaagt gctgctgcaa gaagggtgat gaggaagaga
     1861 acgctcccaa ccccaatcca gatcagaagc cacgtcgaaa gaagaaagaa aagcggccta
     1921 gaggcaccat gcaagctatc aacaatgaga agaaagcttc caaagtcctt ggcattgtat
     1981 tctttgtgtt tctgatcatg tggtgcccgt ttttcatcac caatatcctg tcggtgcttt
     2041 gtgggaaggc ctgtaaccaa aagctaatgg agaaacttct caatgtgttt gtttggattg
     2101 gctatgtgtg ttcaggcatc aatcctctgg tgtacactct cttcaacaaa atttaccgaa
     2161 gggctttctc taaatatttg cgctgcgatt ataagccaga caaaaagcct cctgttcgac
     2221 agattcctag ggttgctgcc actgctttgt ctgggaggga gctcaatgtt aacatttatc
     2281 ggcataccaa tgaacgtgta gttaggaaag ctaatgacac agagcctggc atagagatgc
     2341 aggtagagaa tttagagctg ccggtcaatc cctctaatgt ggtcagcgag aggattagta
     2401 gtgtgacgcg tacgcggccg ctcgagcaga aactcatctc agaagaggat ctggcagcaa
     2461 atgatatcct ggattacaag gatgacgacg ataaggttta aacggccggc cgcggtcata
     2521 gctgtttcct gaacagatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     2581 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     2641 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     2701 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     2761 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     2821 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     2881 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     2941 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     3001 gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca
     3061 cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact
     3121 gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagcg
     3181 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3241 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3301 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3361 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3421 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3481 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3541 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3601 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3661 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3721 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3781 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3841 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3901 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3961 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4021 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc
     4081 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga
     4141 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg
     4201 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
     4261 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     4321 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     4381 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     4441 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     4501 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     4561 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     4621 atatcacggg tagccaacgc tatgtcctga tagcgatccg ccacacccag ccggccacag
     4681 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     4741 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
     4801 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     4861 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     4921 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     4981 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     5041 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     5101 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
     5161 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     5221 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     5281 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt
     5341 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     5401 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     5461 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     5521 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     5581 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     5641 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
     5701 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5761 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5821 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5881 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5941 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6001 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6061 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6121 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6181 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6241 aaatttaacg cgaatttt
//
