LOCUS       V031462                 6524 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031462
VERSION     V031462
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1004..2722
                     /label="Hspd1(NM_001356512)"
                     /note="Hspd1(NM_001356512)"
                     /gene="Hspd1"
     CDS             2735..2764
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     primer_bind     complement(2781..2797)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2808..3430
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3579..4167)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4496..4543
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4775..5569)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5604..5961)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5963..6067)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6094..6522
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccgcc accatgcttc gactacccac
     1021 agtccttcgc cagatgagac cagtgtcccg ggcactggct cctcatctca ctcgggccta
     1081 tgccaaagat gtaaaatttg gtgcggacgc tcgagcctta atgcttcaag gtgtagacct
     1141 tttagcagat gctgtagctg ttacaatggg gccaaaggga agaactgtga ttattgaaca
     1201 gagttgggga agtcccaaag taacaaaaga tggggtcact gttgcaaagt caattgattt
     1261 aaaggataaa tacaaaaata ttggagctaa acttgttcag gacgttgcca ataacacaaa
     1321 cgaagaggct ggggatggca ccaccactgc cactgttctg gcacgatcta ttgccaagga
     1381 gggctttgag aagatcagca aaggggctaa tccagtggaa atccggagag gtgtgatgtt
     1441 ggctgtggat gctgtaattg ctgaacttaa gaaacagtct aaacctgtga caacccctga
     1501 agaaattgct caggttgcta caatttctgc aaatggagac aaagacattg ggaacatcat
     1561 ttctgatgca atgaaaaagg ttggaagaaa gggtgtcatc acagtgaagg atggaaaaac
     1621 cctgaatgat gagctagaaa ttattgaagg catgaagttt gatagaggat atatttcccc
     1681 gtattttatt aacacatcaa aaggtcaaaa gtgtgaattc caagatgcct atgtcttgtt
     1741 gagtgaaaag aaaatttcca gtgttcagtc cattgtccct gctcttgaaa ttgctaatgc
     1801 tcatcggaag ccattggtca taatcgccga agacgttgac ggagaagctc taagcacgct
     1861 ggttttgaac aggctaaaag ttggtcttca ggttgtggca gtcaaagctc caggatttgg
     1921 ggacaatagg aagaaccagc ttaaagatat ggctattgct actggtggtg cagtgtttgg
     1981 agaagagggg ttgaatctaa atcttgaaga tgttcaagct catgacttag gaaaagttgg
     2041 ggaggtcatt gtcaccaaag atgatgccat gcttttgaaa ggaaaaggtg acaaagctca
     2101 cattgaaaaa cgtattcaag aaatcactga gcagctagac atcacaacta gtgaatatga
     2161 aaaagaaaag ctgaacgagc gacttgctaa actttcagat ggagtagctg tgttgaaggt
     2221 tggaggaaca agtgatgttg aagtgaatga gaaaaaagac agagttactg atgctctcaa
     2281 tgctacaaga gcagctgttg aagaaggcat tgttctagga gggggctgcg ctctgcttcg
     2341 gtgcatccca gccttggatt cattaaagcc tgctaatgaa gaccagaaaa taggtataga
     2401 aattattaaa agagcactta aaattcctgc aatgacgatt gctaagaatg caggtgttga
     2461 aggatctttg atagttgaga aaattctgca gagttcctca gaagttggtt atgacgccat
     2521 gcttggagat tttgtgaaca tggtggaaaa agggatcatt gatccaacaa aggttgtgag
     2581 aactgcctta ctggatgctg ctggggtggc ctccttgcta actacagccg aagctgtagt
     2641 gacagaaatt cctaaagaag agaaggaccc tggaatgggt gcaatgggtg gcatgggagg
     2701 gggtatggga ggcggcatgt tcaggcggcc gctcgagcag aaactcatct cagaagagga
     2761 tctgtaaacg gccggccgcg gtcatagctg tttcctgaac agatcccggg tggcatccct
     2821 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     2881 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     2941 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     3001 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     3061 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     3121 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     3181 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     3241 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttaaaataac
     3301 tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc caaccggtgg
     3361 gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact cagtagatgc
     3421 ctgttgaatt gggtacgcgg ccagcggcga gcggtatcag ctcactcaaa ggcggtaata
     3481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4321 taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca
     4381 cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg
     4441 ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca
     4501 aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct
     4561 tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc
     4621 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     4681 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc
     4741 gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     4801 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     4861 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     4921 gatccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     4981 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     5041 tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     5101 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     5161 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     5221 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     5281 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     5341 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca
     5401 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     5461 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     5521 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     5581 ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     5641 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     5701 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     5761 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     5821 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
     5881 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
     5941 agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc atttatcagg
     6001 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6061 ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg
     6121 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6181 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6241 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6301 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6361 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6421 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     6481 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa tttt
//
