LOCUS       V030883                 6072 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030883
VERSION     V030883
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2219
                     /label="Hrh2(NM_001010973)"
                     /note="Hrh2(NM_001010973)"
                     /gene="Hrh2"
     CDS             2238..2267
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2286..2309
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2329..2345)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2356..2978
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3127..3715)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4044..4091
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4323..5117)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5152..5509)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5511..5615)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5642..6070
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagcccaat ggcacggttc attcctgctg tttggactct attgcattga
     1081 aagtcaccat cagtgtggtc ctcaccaccc tcatcttcat cactgttgct ggcaatgtgg
     1141 tcgtctgcct ggctgtcagc ttgaatcgtc ggctccgcag tctgaccaat tgcttcattg
     1201 tgtccctggc agccactgac ctgcttcttg gcctcctggt gatgcccttc tctgccattt
     1261 accagttgtc cttcaagtgg agctttggcc aggtcttctg caacatctac accagcctgg
     1321 atgtgatgct ctgcacagct tccatcctca acctcttcat gatcagcttg gaccgctact
     1381 gcgctgtcac ggacccactg cggtaccccg tgttggtcac ccctgttcgc gttgccatct
     1441 ctttggtctt tatttgggtc atttccatca ccctctcctt cctctctatt cacctaggat
     1501 ggaacagcag aaatgggacc agagggggca atgacacctt caagtgcaaa gtgcaggtca
     1561 acgaggtata tggactggtg gatgggatgg tcaccttcta cctgcctctt ctcatcatgt
     1621 gtgtcaccta ctacagaatc tttaagatcg ccagggagca agccaagagg atcaaccaca
     1681 tcagctcctg gaaggcagcc accatcagag aacacaaagc caccgtgacc ctggctgccg
     1741 tcatgggagc attcatcgtc tgctggtttc cttacttcac tgccttcgtt taccgtggac
     1801 tgagagggga tgacgctgtg aacgaggtgg tcgagggtat cgtcctatgg ttgggttatg
     1861 ccaattcagc cctgaacccc atcctgtacg ccaccctcaa cagagacttc cgcatggcgt
     1921 accaacagct cttccactgc aagcttgcaa gccacaactc tcacaaaact tccctgaggt
     1981 tgaacaactc tctgctgtcc aggagccaaa gccgagaagg tagatggcag gaggagaagc
     2041 ccctgaagct tcaggtgtgg agtgggacag aactcacaca cccccaggga agcccagtca
     2101 ggacacgact gagtcactca agttgtctcc tgagcctgag cctcctgtcc ttcatctgga
     2161 agctgggaac ttggattcat cacaggagac ccttccaacc atcactacat atcagtgcta
     2221 cgcgtacgcg gccgctcgag cagaaactca tctcagaaga ggatctggca gcaaatgata
     2281 tcctggatta caaggatgac gacgataagg tttaaacggc cggccgcggt catagctgtt
     2341 tcctgaacag atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     2401 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     2461 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     2521 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     2581 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     2641 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     2701 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     2761 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     2821 ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat
     2881 aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc
     2941 tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcggcgagc
     3001 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3061 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3121 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3181 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3241 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3301 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3361 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3421 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3481 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3541 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3601 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3661 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3721 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3781 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3841 ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc ctgccgcccc
     3901 gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga
     3961 agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc
     4021 ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct
     4081 ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag
     4141 cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     4201 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     4261 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     4321 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     4381 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     4441 cgggtagcca acgctatgtc ctgatagcga tccgccacac ccagccggcc acagtcgatg
     4501 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     4561 acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc
     4621 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     4681 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     4741 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     4801 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     4861 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     4921 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     4981 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     5041 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     5101 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
     5161 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     5221 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     5281 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     5341 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     5401 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     5461 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
     5521 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5581 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5641 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5701 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5761 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5821 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5881 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5941 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6001 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6061 aacgcgaatt tt
//
