LOCUS       V041411                 9133 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041411
VERSION     V041411
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3792
                     /label="Hip1(NM_146001)"
                     /note="Hip1(NM_146001)"
                     /gene="Hip1"
     CDS             3799..3825
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3829..3855
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3859..3885
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3909..4497
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4563..4611
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4813..5241
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5255..5584
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5651..6445
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6621..6742
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6791..6807)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6815..6831
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6839..6869)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6884..6905
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7193..7781)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7952..8812)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8813..8917)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgga ccgaatggcc
      721 agctccatga agcaggtatc caacccgctg cccaaagtgt tgagccgccg cggagttggt
      781 gctgggatgg aggctgcgga gcgcgagagc ttcgagcgga ctcagacggt cagcgtcaat
      841 aaggccatta atacgcagga agtggccgtg aaagagaaac atgccaggac gtgtattctg
      901 ggcacccacc atgagaaagg agcgcagacc ttttggtctg tggtcaaccg actgcctctc
      961 tccagcaacg ccatgctgtg ctggaagttt tgtcacgtgt tccacaaact tcttcgagat
     1021 ggacatccga acgtgctgaa agactctctg agatacaaaa atgagttaag cgacatgagc
     1081 aggatgtggg gccacctgag cgaggggtat ggacagcttt gcagcatcta cctcaaactg
     1141 ctgagaacaa ggatggagta ccacaccaaa aatcccaggt tcccgggcaa cctccagatg
     1201 agtgaccgac agctggatga ggccggagaa agtgacgtta acaacttttt tcagctcaca
     1261 gtggagatgt ttgattacct ggagtgtgaa ctgaacctct tccagactgt gttcaactcc
     1321 ttggacatgt cccgttcggt gtccgtgacc acggctggcc agtgccgcct cgcaccgctc
     1381 atccaggtca tcctggactg cagtcacctc tatgactaca ctgtcaagct gctcttcaag
     1441 ctccactcct gtcttccagc tgacaccctg cagggccacc gagaccgctt catggagcag
     1501 ttcacaaagt tgaaagatct gtttcagcgc tccagcaacc tacagtactt caagcggctc
     1561 attcagatcc cccagctgcc cgagaatcca cccaacttcc tacgagcctc ggccctgtca
     1621 gagcacatca gtcctgtggt ggtgatcccg gcagaggtgt catccccaga cagtgagcct
     1681 gtcctggaga aggatgacct catggacatg gacgcctccc agcagacttt gtttgacaac
     1741 aagtttgatg acgtctttgg cagctcattg agcagcgacc ctttcaattt caacaatcaa
     1801 aatggcgtga acaaggacga gaaggaccac ttgattgaac gcctgtacag agagatcagt
     1861 ggactgacag ggcagctgga caacatgaag attgagagcc agcgggccat gctgcagctg
     1921 aagggtcgag tgagtgagct ggaggcagag ctagcagagc agcagcactt gggccggcag
     1981 gctatggatg actgcgagtt cctgcgcact gagctggatg aactgaagag gcagcgagag
     2041 gacacggaga aggcacagcg cagcctgact gagatagaaa gaaaggccca ggctaatgaa
     2101 cagaggtata gcaagttaaa agagaagtac agtgaactgg tgcagaacca tgctgacctg
     2161 ctgcggaaga acgcagaggt gaccaaacag gtgtccgtgg cccggcaagc ccaggtggat
     2221 ttggaaagag agaaaaaaga gctagcagat tcctttgcac ggactcaaga gcaacaggat
     2281 gttctagaga acctgaagca tgaactggcc accagcagac aggagctgca ggtcctccac
     2341 agcaacctgg aaacctctgc ccagtcagaa gcgaaatggc tgacacagat cgccgagttg
     2401 gagaaggaac aaggcagctt ggcgactgtt gcagctcaga gagaggaaga gttatcagcc
     2461 ctccgagacc agctggaaag cacccagatc aagctggctg gggcccagga atccatgtgc
     2521 cagcaggtga aggaccagag gaaaaccctc ttggcaggga tcaggaaggc tgcggagcgt
     2581 gagatacagg aggcgctgag ccagcttgag gaacccaccc tcatcagctg tgcaggatcc
     2641 acagatcacc ttctctccaa agtcagctcc gtttccagct gcctcgagca actggaaaag
     2701 aacggcagcc agtatctggc ctgcccagaa gatattagtg agcttctgca ctcgatcacc
     2761 ctgcttgccc acttgaccgg tgacactatc atccagggga gtgccaccag cctccgggcc
     2821 ccaccggagc cagccgactc gctgacggag gcctgtaggc agtatggcag agaaaccctg
     2881 gcctatctgt cctccctgga ggaagaggga actatggaga atgctgacgt cacagccctt
     2941 aggaactgcc tcagcagggt caagaccctt ggcgaggagc tgctgcccag gggcctggac
     3001 atcaagcagg aagagctggg tgacctggtg gacaaggaga tggcagccac ttcagctgcc
     3061 attgaagctg ccaccacccg gatagaggaa attctcagta agtcccgagc aggagacacg
     3121 ggagtcaagc tggaggtgaa tgagaggatc ctgggttcct gtaccagcct gatgcaggcc
     3181 atcaaggtgc tcgttgtggc ctccaaggac ctccagaagg agatagtgga gagtggcagg
     3241 ggtactgcat cccctaaaga attttacgcc aagaactctc ggtggacgga agggctgata
     3301 tccgcctcca aagctgttgg ttggggagct accatcatgg tggatgctgc tgatcttgtg
     3361 gtccaaggca aagggaagtt cgaggagctg atggtgtgtt cacgcgagat tgctgccagt
     3421 actgcccagc tcgtggctgc atccaaggtg aaagcgaaca agggcagcct caatctgacc
     3481 cagctgcagc aggcctctcg aggagtgaac caggccacag ccgctgtggt ggcctcaacc
     3541 atttctggca aatctcagat tgaggaaaca gacagtatgg acttctcaag catgacactg
     3601 acccagatca agcgccagga gatggattcc caggttaggg tgctggagct ggaaaatgac
     3661 ctgcagaagg agcgtcagaa actaggagag ctacggaaga aacactacga gctggcgggc
     3721 gtggctgagg gctgggagga agggacagaa gcatcaccgt ctactgtcca agaagcaata
     3781 ccggacaaag agctcgagta cccatacgac gtacctgatt atgcaactta tccttatgat
     3841 gtacctgatt atgctagcta tccttatgat gtcccagact acgcatagtg agggcccgat
     3901 atctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3961 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4021 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4081 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4141 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4201 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4261 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     4321 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4381 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4441 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     4501 gaaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     4561 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     4621 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     4681 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     4741 tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta
     4801 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4861 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4921 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag
     4981 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5041 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5101 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5161 cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt
     5221 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     5281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     5341 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5401 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5461 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5521 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5581 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     5641 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5701 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5761 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5821 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5881 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5941 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     6001 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6061 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6121 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     6181 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     6241 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     6301 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6361 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6421 tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag
     6481 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6541 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6601 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6661 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6721 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     6781 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6841 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6901 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6961 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7021 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7081 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7141 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7201 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7261 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7321 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7381 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7441 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7501 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7561 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7621 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7681 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7741 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7801 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7861 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7921 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7981 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8041 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8101 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8161 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8221 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8281 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8341 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8401 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8461 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8521 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8581 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8641 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8701 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8761 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8821 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8881 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8941 ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca gtacaatctg
     9001 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     9061 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     9121 gaatctgctt agg
//
