LOCUS       V046185                12342 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046185
VERSION     V046185
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..7022
                     /label="Hcfc1(NM_001411234)"
                     /note="Hcfc1(NM_001411234)"
                     /gene="Hcfc1"
     CDS             7029..7094
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    7118..7706
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7772..7820
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8022..8450
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8464..8793
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8860..9654
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9830..9951
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10000..10016)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10024..10040
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10048..10078)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10093..10114
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10402..10990)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11161..12021)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12022..12126)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatggc ttcggctgtg tctcccgcaa
      781 acttgccagc ggtgcttctg cagccccgct ggaaacgggt ggtgggctgg tcgggtcccg
      841 tgccccgacc ccgccacggc caccgtgcag tggctatcaa ggagcttata gtggtgtttg
      901 gcggcggcaa cgaggggata gtggacgaac tacacgtgta caacactgca accaaccagt
      961 ggttcatccc agctgtgaga ggggatatcc ctccagggtg tgcagcctat ggctttgtgt
     1021 gtgatggtac tcgcctattg gtgtttggtg gaatggtaga gtatggaaaa tacagcaacg
     1081 acctctatga actccaggca agtcgttggg aatggaagag actgaaggca aagacaccca
     1141 aaaatgggcc tcctccatgt cctcggcttg gacatagctt ctcccttgtg ggcaacaaat
     1201 gttacctgtt tgggggtctg gccaatgata gtgaggaccc caagaacaac attccgaggt
     1261 acctgaatga cttatatatt ctcgaactac ggccaggctc tggagtggta gcttgggaca
     1321 tccccatcac ttacggtgtc ctgcctccac cccgggagtc acatactgct gtggtctaca
     1381 ctgaaaaaga taacaagaaa tccaagctgg tgatctatgg agggatgagt ggctgcaggc
     1441 taggggacct ttggaccctg gacattgaga cactgacatg gaataagccc agccttagtg
     1501 gggtggcacc ccttcctcgc agcctccact ctgcaaccac cataggaaac aaaatgtatg
     1561 tatttggtgg ctgggtgccc cttgtcatgg acgatgtcaa agtggccaca cacgagaagg
     1621 agtggaagtg taccaacaca ctggcttgtc tcaacctgga taccatggcc tgggaaacca
     1681 tcctgatgga tacattggag gacaacattc ctcgagctcg agcaggccac tgtgctgttg
     1741 ccatcaatac tcgtctgtat atttggagtg gccgtgatgg ctaccgcaag gcctggaaca
     1801 accaggtctg ctgcaaggac ttgtggtatt tggagacaga aaagccacca cccccagccc
     1861 gagtacaact agtacgagcc aacaccaact cactggaggt tagctggggt gcagtggcaa
     1921 cagctgacag ttaccttcta caactccaga aatatgacat tcctgccaca gctgctacgg
     1981 ctacctcccc cactcccaat ccagtcccgt ctgtgcctgc caaccctccc aagagccctg
     2041 cgccagcagc agctgcacct gctgtacagc cactgaccca agtaggcatc acacttgtgc
     2101 cccaggctgc cactgcaccc ccaagcacaa ccaccatcca ggtcttgccg acagtgccag
     2161 gcagctccat ttctgtgccc actgcagcca ggactcaagg tgtccctgct gttctcaaag
     2221 tgactggtcc tcaagctaca acaggaacac cactggttac catgagacct gcaagccagg
     2281 ctggaaaagc tcctgtcact gtgacttccc tgcctgccag tgttcgaatg gttgtaccca
     2341 cacagagtgc ccaggggacg gtgatcggca gcaacccaca gatgagtggg atggccgcat
     2401 tggctgctgc tgctgctgcc acacagaaaa tccctccatc ctcagcaccc acggtgctga
     2461 gtgtcccagc agggaccacc atcgtcaaga cagtggctgt gacacctggc acgaccactc
     2521 ttccagccac tgtgaaggtg gcctcctccc ctgtcatggt gagcaaccca gccactcgaa
     2581 tgctaaagac tgcagctgcc caagtgggga catctgtgtc ctctgctgcc aacacatcta
     2641 ctcgccctat catcacagta cacaaatcag gaactgtaac agtggcccag caagcccagg
     2701 tggtgaccac ggtggtaggt ggagtcacca agaccatcac cctagtgaag agccccatct
     2761 ctgtcccagg aggcagtgct ctgatttcca atctgggaaa agtgatgtcg gtggtccaga
     2821 ccaaaccagt tcagacatca gcagtgacag gccaagcatc tacaggtcct gtgactcaga
     2881 tcatccagac caaaggaccc ctgccagcgg ggactatcct gaagctggtg acatcagcag
     2941 atggcaagcc cacaaccatc attaccacca cacaggctag tggggcaggg accaagccca
     3001 ctatcctggg catcagtagt gtttctccca gcaccaccaa acctggcaca actaccatta
     3061 ttaagaccat tcctatgtcg gccattatca cccaggcagg tgccacaggt gttaccagca
     3121 gtcctggcat taagtcccca attacaatta tcaccaccaa agtgatgact tcaggaacag
     3181 gagcgcctgc taaaatcatc actgctgtcc ccaagattgc tactggccat gggcaacaag
     3241 gagtgaccca ggtggtgcta aagggggccc ctggacaacc aggcaccatc ctccgtactg
     3301 tgcctatggg cggcgttcgc ctggtcaccc ctgtcaccgt ctctgctgtc aagccagctg
     3361 tcaccacatt ggttgtgaag ggtaccacag gtgttacaac gctaggcaca gtgacaggca
     3421 ctgtctccac cagcctggcc ggagctgggg cacatagcac cagtgcttcc ctggctacac
     3481 ctatcactac cttgggcact attgctacgc tctcaagcca ggtgatcaac cctactgcta
     3541 tcacagtgtc agctgcacag actacactaa cagctgctgg tgggcttacc acacccacaa
     3601 tcacaatgca gcctgtctcc cagcctaccc aggtcactct gattacagca cccagtgggg
     3661 ttgaagcaca gcctgtacat gaccttcctg tatccatttt ggcctcacct actacagagc
     3721 agcccacagc aacagtcacc atcgctgact caggccaggg tgatgtgcag cccggcactg
     3781 tgacactggt gtgttccaac ccaccctgtg aaacccatga aacaggcacc accaacacag
     3841 ctaccaccac tgttgtggct aaccttgggg gacatcctca acctacccag gtgcagtttg
     3901 tttgtgacag acaggagaca gctgcttcac ttgtgacctc agctgtagga caacagaatg
     3961 gtaatgtggt ccgtgtctgt tcaaaccccc cctgtgagac ccatgagacg ggcactacca
     4021 acactgccac aacagccacc tccaacatgg ctgggcagca tggctgctcg aaccccccct
     4081 gtgagactca tgagacaggc accaccagca ctgccactac agcaatgtcc agcatgggca
     4141 ctgggcagca gcgagacact cgtcgtacca ctaacacccc cactgtagtg cggatcactg
     4201 tggctcctgg ggcattggag agagtccagg gtaccgtgaa gcctcagtgc caaacccagc
     4261 agaccaacat gaccaccacc accatgactg tgcaggccac tggagctcca tgctcagctg
     4321 gccccctgct taggccaagt gtggcactgg agtctgggag ccacagccct gcctttgtgc
     4381 aactagccct tccaagtgtc agagttgggc taagtggccc cagcagcaag gacatgccca
     4441 cagggcgcca accagagaca tatcatactt acacaactaa taccccaacc acaacccgct
     4501 ctatcatggt tgctggggag cttggtgcag ctcgggtggt ccccacatct acatatgaga
     4561 gcctccaggc aagctctcct agcagcacca tgactatgac agccctagag gcactgctgt
     4621 gcccttcggc tactgtcacc caagtctgct ccaacccgcc atgtgagacc catgagacgg
     4681 gtaccaccaa caccgccact acctccaatg cgggcagtgc tcagcgagta tgctccaacc
     4741 cgccttgtga gactcatgag acgggcacca cacacacagc taccactgcc acatcaaatg
     4801 gaggtgcagg ccagcctgag ggtggacaac agcctgccag tggccatccc tgcgagacac
     4861 accagaccac ttccactggc accactatgt cagtcagtgt gggtaccctg attcctgatg
     4921 ctacttcctc tcatggaacc ctggagtcgg gcttagaggt ggtagcagtg cccactgtca
     4981 cctcccaggc tggttccaca ttgctggcct ctttcccaac acagagggta tgctccaacc
     5041 ctccttgcga gacccacgag acaggtacca cgcacacagc caccactgtc acctctaaca
     5101 tgagctcaaa ccaagaccct ccaccagctg ccagtgacca aggagaggtg gcaagcaccc
     5161 aaggtgacag cacaaatatc accagtgcca gtgctatcac tacaagtgtg tcttctacat
     5221 tgccacgagc agtgaccact gtgacacagt ctacaccagt ccctggtccc tctgtgccga
     5281 acatctcatc attgactgag actaccccag gggctctgaa ttccgaagtc cccatcccag
     5341 ccacgataac agtgaccata gccaacacag aaacttctga catgcccttc tctgctgttg
     5401 acatcctgca gcccccagag gaactccagg tctcaccagg gcctcgccag cagctgcctc
     5461 cacggcaact cctgcagtct gcctccacac ccctgatggg ggagtctact gaggtcctgt
     5521 cagcctccca gacccctgag ctccaggccg ccgtggatct gagcagcact ggggacccat
     5581 cttcaggcca ggagcctacc acctctgctg tcgtggccac tgtggtggtc caaccacccc
     5641 cacccacaca gtctgaagta gaccagttat cacttcccca agagctgatg gctgaagccc
     5701 aggcgggcac cacaaccctt atggtaacag ggctcactcc agaggagctg gcagtgactg
     5761 ctgctgctga agcagctgct caagctgcag ccactgaaga agcccaagcc ttggccatcc
     5821 aggctgtgct ccaggccgca cagcaggctg tcatgggcac tggggagccc atggatacat
     5881 ctgaagcagc agcagcagtg acacaagcag aactgggtca cctttcagct gaaggccaag
     5941 agggtcaggc taccaccata cccattgtgt tgacacagca ggagcttgca gccctggtgc
     6001 agcagcagca gcagctccag gaggctcaag ctcaagccca gcaacagcac catcttccca
     6061 ctgaggctct ggccccagct gacagtctca atgacccatc catcgagagc aactgcctca
     6121 acgagttagc tagtgctgtc ccaagcaccg tggctttgct accctcaaca gctaccgaga
     6181 gcctggctcc atctaacaca tttgtggctc cccagcctgt tgtagctagt ccagcaaaga
     6241 tgcaggctgc agctaccctt actgaagtgg ccaatggcat tgagtccctg ggtgtgaaac
     6301 cggacttgcc acccccaccc agcaaagccc ctgtgaaaaa ggagaaccag tggtttgatg
     6361 tgggggtcat taagggtacc agtgtaatgg tgacacacta ttttctgcca ccagatgatg
     6421 ctgttcagtc agatgatgac tcaggcacgg tcccagacta taaccagcta aagaagcagg
     6481 agctacagcc aggcacggct tacaaatttc gagttgctgg aatcaatgct tgtggccggg
     6541 gacccttcag tgagatctca gcctttaaga cttgtctgcc tgggttccca ggggctcctt
     6601 gtgctattaa aatcagcaag agcccagatg gtgctcacct cacctgggag ccaccgtctg
     6661 tgacctccgg caagatcatc gagtactctg tgtacctggc catccagagc tcacaggcca
     6721 gtggtgagcc aaagagctcc accccagccc agctggcctt catgcgagtg tactgtgggc
     6781 ctagcccttc ctgcctagtg cagtcctcca gcctctccaa cgcccacatt gactatacta
     6841 caaagcctgc catcatcttc cgcattgctg cccgcaatga aaagggctac ggccctgcca
     6901 cacaagtgag gtggttgcaa gaaactagta aagacagctc tggcaccaag ccggccagca
     6961 agcggcccat gtcgtctcca gaaatgaaat ctgctccaaa gaagtctaag gctgatggtc
     7021 agggatccga ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg
     7081 atgacgatga caagtagtga gggcccgata tctcgacaat caacctctgg attacaaaat
     7141 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7201 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7261 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7321 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7381 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7441 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7501 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     7561 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7621 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     7681 gatctccctt tgggccgcct ccccgcctgg aaacggggga ggctaactga aacacggaag
     7741 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     7801 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     7861 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     7921 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     7981 catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     8041 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     8101 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     8161 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga
     8221 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     8281 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     8341 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc
     8401 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     8461 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8521 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8581 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8641 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8701 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     8761 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     8821 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     8881 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     8941 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     9001 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     9061 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     9121 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     9181 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     9241 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     9301 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     9361 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     9421 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     9481 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     9541 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     9601 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     9661 ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     9721 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     9781 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     9841 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9901 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9961 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
    10021 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    10081 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    10141 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    10201 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    10261 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    10321 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    10381 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    10441 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    10501 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    10561 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
    10621 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    10681 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    10741 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10801 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    10861 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10921 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10981 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    11041 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    11101 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    11161 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    11221 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    11281 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    11341 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    11401 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    11461 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    11521 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    11581 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    11641 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    11701 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    11761 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    11821 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    11881 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    11941 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    12001 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    12061 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    12121 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    12181 cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
    12241 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
    12301 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gg
//
