LOCUS       V031071                 5130 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031071
VERSION     V031071
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1277
                     /label="Hamp(NM_032541)"
                     /note="Hamp(NM_032541)"
                     /gene="Hamp"
     CDS             1296..1325
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1344..1367
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1387..1403)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1414..2036
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2185..2773)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3102..3149
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3381..4175)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4210..4567)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4569..4673)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      4700..5128
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcactcagc actcggaccc aggctgcctg tctcctgctt ctcctccttg
     1081 ccagcctgag cagcaccacc tatctccatc aacagatgag acagactaca gagctgcagc
     1141 ctttgcacgg ggaagaaagc agggcagaca ttgcgatacc aatgcagaag agaaggaaga
     1201 gagacaccaa cttccccatc tgcatcttct gctgtaaatg ctgtaacaat tcccagtgtg
     1261 gtatctgttg caaaacaacg cgtacgcggc cgctcgagca gaaactcatc tcagaagagg
     1321 atctggcagc aaatgatatc ctggattaca aggatgacga cgataaggtt taaacggccg
     1381 gccgcggtca tagctgtttc ctgaacagat cccgggtggc atccctgtga cccctcccca
     1441 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     1501 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     1561 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     1621 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     1681 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     1741 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     1801 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     1861 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag
     1921 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc
     1981 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt
     2041 acgcggccag cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2101 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2161 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2221 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2281 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2341 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2401 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2461 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2521 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2581 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     2641 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2701 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2761 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2821 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2881 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat
     2941 ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     3001 gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     3061 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     3121 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
     3181 cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc
     3241 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     3301 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     3361 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     3421 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     3481 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcgatc cgccacaccc
     3541 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     3601 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     3661 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     3721 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     3781 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     3841 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     3901 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     3961 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     4021 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     4081 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     4141 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     4201 tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     4261 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     4321 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     4381 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     4441 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     4501 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc
     4561 gcctcaggac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4621 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4681 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4741 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4801 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4861 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4921 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4981 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5041 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5101 ctgatttaac aaaaatttaa cgcgaatttt
//
