LOCUS       V043162                 8455 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043162
VERSION     V043162
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3114
                     /label="HSP90B1(NM_003299)"
                     /note="HSP90B1(NM_003299)"
                     /gene="HSP90B1"
     CDS             3121..3147
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3151..3177
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3181..3207
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3231..3819
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3885..3933
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4135..4563
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4577..4906
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4973..5767
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5943..6064
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6113..6129)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6137..6153
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6161..6191)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6206..6227
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6515..7103)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7274..8134)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8135..8239)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgag ggccctgtgg
      721 gtgctgggcc tctgctgcgt cctgctgacc ttcgggtcgg tcagagctga cgatgaagtt
      781 gatgtggatg gtacagtaga agaggatctg ggtaaaagta gagaaggatc aaggacggat
      841 gatgaagtag tacagagaga ggaagaagct attcagttgg atggattaaa tgcatcacaa
      901 ataagagaac ttagagagaa gtcggaaaag tttgccttcc aagccgaagt taacagaatg
      961 atgaaactta tcatcaattc attgtataaa aataaagaga ttttcctgag agaactgatt
     1021 tcaaatgctt ctgatgcttt agataagata aggctaatat cactgactga tgaaaatgct
     1081 ctttctggaa atgaggaact aacagtcaaa attaagtgtg ataaggagaa gaacctgctg
     1141 catgtcacag acaccggtgt aggaatgacc agagaagagt tggttaaaaa ccttggtacc
     1201 atagccaaat ctgggacaag cgagttttta aacaaaatga ctgaagcaca ggaagatggc
     1261 cagtcaactt ctgaattgat tggccagttt ggtgtcggtt tctattccgc cttccttgta
     1321 gcagataagg ttattgtcac ttcaaaacac aacaacgata cccagcacat ctgggagtct
     1381 gactccaatg aattttctgt aattgctgac ccaagaggaa acactctagg acggggaacg
     1441 acaattaccc ttgtcttaaa agaagaagca tctgattacc ttgaattgga tacaattaaa
     1501 aatctcgtca aaaaatattc acagttcata aactttccta tttatgtatg gagcagcaag
     1561 actgaaactg ttgaggagcc catggaggaa gaagaagcag ccaaagaaga gaaagaagaa
     1621 tctgatgatg aagctgcagt agaggaagaa gaagaagaaa agaaaccaaa gactaaaaaa
     1681 gttgaaaaaa ctgtctggga ctgggaactt atgaatgata tcaaaccaat atggcagaga
     1741 ccatcaaaag aagtagaaga agatgaatac aaagctttct acaaatcatt ttcaaaggaa
     1801 agtgatgacc ccatggctta tattcacttt actgctgaag gggaagttac cttcaaatca
     1861 attttatttg tacccacatc tgctccacgt ggtctgtttg acgaatatgg atctaaaaag
     1921 agcgattaca ttaagctcta tgtgcgccgt gtattcatca cagacgactt ccatgatatg
     1981 atgcctaaat acctcaattt tgtcaagggt gtggtggact cagatgatct ccccttgaat
     2041 gtttcccgcg agactcttca gcaacataaa ctgcttaagg tgattaggaa gaagcttgtt
     2101 cgtaaaacgc tggacatgat caagaagatt gctgatgata aatacaatga tactttttgg
     2161 aaagaatttg gtaccaacat caagcttggt gtgattgaag accactcgaa tcgaacacgt
     2221 cttgctaaac ttcttaggtt ccagtcttct catcatccaa ctgacattac tagcctagac
     2281 cagtatgtgg aaagaatgaa ggaaaaacaa gacaaaatct acttcatggc tgggtccagc
     2341 agaaaagagg ctgaatcttc tccatttgtt gagcgacttc tgaaaaaggg ctatgaagtt
     2401 atttacctca cagaacctgt ggatgaatac tgtattcagg cccttcccga atttgatggg
     2461 aagaggttcc agaatgttgc caaggaagga gtgaagttcg atgaaagtga gaaaactaag
     2521 gagagtcgtg aagcagttga gaaagaattt gagcctctgc tgaattggat gaaagataaa
     2581 gcccttaagg acaagattga aaaggctgtg gtgtctcagc gcctgacaga atctccgtgt
     2641 gctttggtgg ccagccagta cggatggtct ggcaacatgg agagaatcat gaaagcacaa
     2701 gcgtaccaaa cgggcaagga catctctaca aattactatg cgagtcagaa gaaaacattt
     2761 gaaattaatc ccagacaccc gctgatcaga gacatgcttc gacgaattaa ggaagatgaa
     2821 gatgataaaa cagttttgga tcttgctgtg gttttgtttg aaacagcaac gcttcggtca
     2881 gggtatcttt taccagacac taaagcatat ggagatagaa tagaaagaat gcttcgcctc
     2941 agtttgaaca ttgaccctga tgcaaaggtg gaagaagagc ccgaagaaga acctgaagag
     3001 acagcagaag acacaacaga agacacagag caagacgaag atgaagaaat ggatgtggga
     3061 acagatgaag aagaagaaac agcaaaggaa tctacagctg aaaaagatga attgctcgag
     3121 tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga ttatgctagc
     3181 tatccttatg atgtcccaga ctacgcatag tgagggcccg atatctcgac aatcaacctc
     3241 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3301 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3361 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     3421 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     3481 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     3541 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     3601 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     3661 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     3721 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     3781 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaaacggg ggaggctaac
     3841 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     3901 gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     3961 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     4021 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     4081 cggcaggccc tgccatagca gatctgcgca gctggggctc tagggggtat ccccacgcgc
     4141 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4201 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4261 ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt
     4321 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4381 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4441 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4501 ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4561 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4621 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4681 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4741 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4801 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     4861 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     4921 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     4981 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5041 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5101 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     5161 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5221 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5281 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5341 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5401 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5461 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     5521 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5581 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5641 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5701 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5761 cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc aacctgccat
     5821 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5881 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5941 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6001 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6061 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     6121 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6181 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6241 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6301 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6361 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6421 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6481 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6541 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6601 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6661 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     6721 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6781 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6841 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6901 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     6961 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7021 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7081 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7141 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7201 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7261 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7321 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7381 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7441 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7501 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7561 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7621 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7681 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7741 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7801 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7861 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7921 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7981 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8041 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8101 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8161 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8221 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     8281 gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg ccgcatagtt
     8341 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     8401 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagg
//
