LOCUS       V042987                 8242 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042987
VERSION     V042987
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2901
                     /label="HSP90AA1(NM_005348)"
                     /note="HSP90AA1(NM_005348)"
                     /gene="HSP90AA1"
     CDS             2908..2934
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2938..2964
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2968..2994
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3018..3606
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3672..3720
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3922..4350
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4364..4693
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4760..5554
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5730..5851
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5900..5916)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5924..5940
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5948..5978)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5993..6014
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6302..6890)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7061..7921)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7922..8026)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgcc tgaggaaacc
      721 cagacccaag accaaccgat ggaggaggag gaggttgaga cgttcgcctt tcaggcagaa
      781 attgcccagt tgatgtcatt gatcatcaat actttctact cgaacaaaga gatctttctg
      841 agagagctca tttcaaattc atcagatgca ttggacaaaa tccggtatga aagcttgaca
      901 gatcccagta aattagactc tgggaaagag ctgcatatta accttatacc gaacaaacaa
      961 gatcgaactc tcactattgt ggatactgga attggaatga ccaaggctga cttgatcaat
     1021 aaccttggta ctatcgccaa gtctgggacc aaagcgttca tggaagcttt gcaggctggt
     1081 gcagatatct ctatgattgg ccagttcggt gttggttttt attctgctta tttggttgct
     1141 gagaaagtaa ctgtgatcac caaacataac gatgatgagc agtacgcttg ggagtcctca
     1201 gcagggggat cattcacagt gaggacagac acaggtgaac ctatgggtcg tggaacaaaa
     1261 gttatcctac acctgaaaga agaccaaact gagtacttgg aggaacgaag aataaaggag
     1321 attgtgaaga aacattctca gtttattgga tatcccatta ctctttttgt ggagaaggaa
     1381 cgtgataaag aagtaagcga tgatgaggct gaagaaaagg aagacaaaga agaagaaaaa
     1441 gaaaaagaag agaaagagtc ggaagacaaa cctgaaattg aagatgttgg ttctgatgag
     1501 gaagaagaaa agaaggatgg tgacaagaag aagaagaaga agattaagga aaagtacatc
     1561 gatcaagaag agctcaacaa aacaaagccc atctggacca gaaatcccga cgatattact
     1621 aatgaggagt acggagaatt ctataagagc ttgaccaatg actgggaaga tcacttggca
     1681 gtgaagcatt tttcagttga aggacagttg gaattcagag cccttctatt tgtcccacga
     1741 cgtgctcctt ttgatctgtt tgaaaacaga aagaaaaaga acaacatcaa attgtatgta
     1801 cgcagagttt tcatcatgga taactgtgag gagctaatcc ctgaatatct gaacttcatt
     1861 agaggggtgg tagactcgga ggatctccct ctaaacatat cccgtgagat gttgcaacaa
     1921 agcaaaattt tgaaagttat caggaagaat ttggtcaaaa aatgcttaga actctttact
     1981 gaactggcgg aagataaaga gaactacaag aaattctatg agcagttctc taaaaacata
     2041 aagcttggaa tacacgaaga ctctcaaaat cggaagaagc tttcagagct gttaaggtac
     2101 tacacatctg cctctggtga tgagatggtt tctctcaagg actactgcac cagaatgaag
     2161 gagaaccaga aacatatcta ttatatcaca ggtgagacca aggaccaggt agctaactca
     2221 gcctttgtgg aacgtcttcg gaaacatggc ttagaagtga tctatatgat tgagcccatt
     2281 gatgagtact gtgtccaaca gctgaaggaa tttgagggga agactttagt gtcagtcacc
     2341 aaagaaggcc tggaacttcc agaggatgaa gaagagaaaa agaagcagga agagaaaaaa
     2401 acaaagtttg agaacctctg caaaatcatg aaagacatat tggagaaaaa agttgaaaag
     2461 gtggttgtgt caaaccgatt ggtgacatct ccatgctgta ttgtcacaag cacatatggc
     2521 tggacagcaa acatggagag aatcatgaaa gctcaagccc taagagacaa ctcaacaatg
     2581 ggttacatgg cagcaaagaa acacctggag ataaaccctg accattccat tattgagacc
     2641 ttaaggcaaa aggcagaggc tgataagaac gacaagtctg tgaaggatct ggtcatcttg
     2701 ctttatgaaa ctgcgctcct gtcttctggc ttcagtctgg aagatcccca gacacatgct
     2761 aacaggatct acaggatgat caaacttggt ctgggtattg atgaagatga ccctactgct
     2821 gatgatacca gtgctgctgt aactgaagaa atgccacccc ttgaaggaga tgacgacaca
     2881 tcacgcatgg aagaagtaga cctcgagtac ccatacgacg tacctgatta tgcaacttat
     2941 ccttatgatg tacctgatta tgctagctat ccttatgatg tcccagacta cgcatagtga
     3001 gggcccgata tctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3061 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3121 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3181 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3241 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3301 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3361 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     3421 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     3481 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     3541 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3601 ccccgcctgg aaacggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     3661 ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc
     3721 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     3781 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     3841 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcagat ctgcgcagct
     3901 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3961 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4021 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca
     4081 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4141 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4201 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4261 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg
     4321 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4381 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4441 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4501 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4561 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4621 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4681 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     4741 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     4801 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     4861 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     4921 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     4981 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5041 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5101 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5161 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5221 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5281 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     5341 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5401 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5461 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5521 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga
     5581 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     5641 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5701 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     5761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     5881 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5941 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6001 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6061 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6121 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6181 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6241 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6301 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6361 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6421 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6481 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6541 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6601 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6661 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6721 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     6781 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6841 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6901 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6961 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7021 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7081 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7141 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7201 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7261 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7321 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7381 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7441 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7501 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7561 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7621 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7681 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7741 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7801 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7861 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7921 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7981 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8041 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt cgactctcag
     8101 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     8161 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     8221 ttgcatgaag aatctgctta gg
//
