LOCUS       V013993                 8201 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013993
VERSION     V013993
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             683..2881
                     /gene="HIC1"
                     /label="Hypermethylated in cancer 1 protein"
                     /note="Hypermethylated in cancer 1 protein from Homo
                     sapiens. Accession#: Q14526"
     CDS             2930..2953
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    2977..3565
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3631..3679
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3881..4309
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4323..4652
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4719..5510
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5689..5822
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5859..5875)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5883..5899)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5907..5937)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5952..5973)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6261..6849)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7023..7880)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7881..7985)
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatgacctt cccggaagct gacatcctgc tgaaatctgg
      721 tgaatgcgct ggtcagacca tgctggacac catggaagct ccgggtcact ctcgtcagct
      781 gctgctgcag ctgaacaacc agcgtaccaa aggtttcctg tgcgacgtta tcatcgttgt
      841 tcagaacgct ctgttccgtg ctcacaaaaa cgttctggct gcttcttctg cttacctgaa
      901 atctctggtt gttcacgaca acctgctgaa cctggaccac gacatggttt ctccggctgt
      961 tttccgtctg gttctggact tcatctacac cggtcgtctg gctgacggtg ctgaagctgc
     1021 tgctgctgct gctgttgctc cgggtgctga accgtctctg ggtgctgttc tggctgctgc
     1081 ttcttacctg cagatcccgg acctggttgc tctgtgcaaa aaacgtctga aacgtcacgg
     1141 taaatactgc cacctgcgtg gtggtggtgg tggtggtggt ggttacgctc cgtacggtcg
     1201 tccgggtcgt ggtctgcgtg ctgctacccc ggttatccag gcttgctacc cgtctccggt
     1261 tggtccgccg ccgccgccgg ctgctgaacc gccgtctggt ccggaagctg ctgttaacac
     1321 ccactgcgct gaactgtacg cttctggtcc gggtccggct gctgctctgt gcgcttctga
     1381 acgtcgttgc tctccgctgt gcggtctgga cctgtctaaa aaatctccgc cgggttctgc
     1441 tgctccggaa cgtccgctgg ctgaacgtga actgccgccg cgtccggact ctccgccgtc
     1501 tgctggtccg gctgcttaca aagaaccgcc gctggctctg ccgtctctgc cgccgctgcc
     1561 gttccagaaa ctggaagaag ctgctccgcc gtctgacccg ttccgtggtg gttctggttc
     1621 tccgggtccg gaaccgccgg gtcgtccgga cggtccgtct ctgctgtacc gttggatgaa
     1681 acacgaaccg ggtctgggtt cttacggtga cgaactgggt cgtgaacgtg gttctccgtc
     1741 tgaacgttgc gaagaacgtg gtggtgacgc tgctgtttct ccgggtggtc cgccgctggg
     1801 tctggctccg ccgccgcgtt acccgggttc tctggacggt ccgggtgctg gtggtgacgg
     1861 tgacgactac aaatcttctt ctgaagaaac cggttcttct gaagacccgt ctccgccggg
     1921 tggtcacctg gaaggttacc cgtgcccgca cctggcttac ggtgaaccgg aatctttcgg
     1981 tgacaacctg tacgtttgca tcccgtgcgg taaaggtttc ccgtcttctg aacagctgaa
     2041 cgctcacgtt gaagctcacg ttgaagaaga agaagctctg tacggtcgtg ctgaagctgc
     2101 tgaagttgct gctggtgctg ctggtctggg tccgccgttc ggtggtggtg gtgacaaagt
     2161 tgctggtgct ccgggtggtc tgggtgaact gctgcgtccg taccgttgcg cttcttgcga
     2221 caaatcttac aaagacccgg ctaccctgcg tcagcacgaa aaaacccact ggctgacccg
     2281 tccgtacccg tgcaccatct gcggtaaaaa attcacccag cgtggtacca tgacccgtca
     2341 catgcgttct cacctgggtc tgaaaccgtt cgcttgcgac gcttgcggta tgcgtttcac
     2401 ccgtcagtac cgtctgaccg aacacatgcg tatccactct ggtgaaaaac cgtacgaatg
     2461 ccaggtttgc ggtggtaaat tcgctcagca gcgtaacctg atctctcaca tgaaaatgca
     2521 cgctgttggt ggtgctgctg gtgctgctgg tgctctggct ggtctgggtg gtctgccggg
     2581 tgttccgggt ccggacggta aaggtaaact ggacttcccg gaaggtgttt tcgctgttgc
     2641 tcgtctgacc gctgaacagc tgtctctgaa acagcaggac aaagctgctg ctgctgaact
     2701 gctggctcag accacccact tcctgcacga cccgaaagtt gctctggaat ctctgtaccc
     2761 gctggctaaa ttcaccgctg aactgggtct gtctccggac aaagctgctg aagttctgtc
     2821 tcagggtgct cacctggctg ctggtccgga cggtcgtacc atcgaccgtt tctctccgac
     2881 cctcgaggac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga
     2941 tgacgatgac aagtagtgag ggcccgatat ctcgacaatc aacctctgga ttacaaaatt
     3001 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3061 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3121 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3181 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3241 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3301 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3361 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     3421 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3481 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3541 atctcccttt gggccgcctc cccgcctgga aacgggggag gctaactgaa acacggaagg
     3601 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     3661 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     3721 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     3781 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     3841 atagcagatc tgcgcagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     3901 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3961 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4021 caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4081 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4141 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4201 acaacactca accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg
     4261 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     4321 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4381 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4441 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4501 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4561 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     4621 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     4681 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     4741 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4801 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4861 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     4921 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4981 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     5041 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     5101 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     5161 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     5221 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     5281 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5341 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5401 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     5461 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5521 tctggggttc gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     5581 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     5641 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     5701 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5761 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     5821 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     5881 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5941 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6001 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6061 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6241 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6421 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     6481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6661 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     6721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7021 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7081 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7141 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7201 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7261 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7321 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7381 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7441 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7501 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7561 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7621 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7681 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7741 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7801 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7861 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7921 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7981 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     8041 ccctatggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     8101 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     8161 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag g
//
