LOCUS       V018902                 9095 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018902
VERSION     V018902
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             683..3754
                     /label="HERC5(NM_016323)"
                     /note="HERC5(NM_016323)"
                     /gene="HERC5"
     CDS             3761..3787
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3791..3817
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3821..3847
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3871..4459
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4525..4573
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4775..5203
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5217..5546
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5613..6407
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6583..6704
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6753..6769)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6777..6793
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6801..6831)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6846..6867
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7155..7743)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7914..8774)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8775..8879)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatggagcg gaggtcgcgg aggaagtcgc ggcgcaacgg
      721 gcgctcgacc gcgggcaagg ccgccgcgac ccagcccgcg aagtctccgg gcgcacagct
      781 ctggctcttt cccagcgccg cgggcctcca ccgcgcgctg ctccggaggg tggaggtgac
      841 gcgccaactc tgctgctcgc cggggcgcct cgcggtcttg gaacgcggcg gggcgggcgt
      901 ccaggttcac cagctgctcg ccgggagcgg cggcgcccgg acgccgaaat gcattaaatt
      961 aggaaaaaac atgaagatac attccgtgga ccaaggagca gagcacatgc tgattctctc
     1021 atcagatgga aaaccatttg agtatgacaa ctatagcatg aaacatctaa ggtttgaaag
     1081 cattttacaa gaaaaaaaaa taattcagat cacatgtgga gattaccatt ctcttgcact
     1141 ctcaaaaggt ggtgagcttt ttgcctgggg acagaacctg catgggcagc ttggagttgg
     1201 aaggaaattt ccctcaacca ccacaccaca gattgtggag cacctcgcag gagtaccctt
     1261 ggctcagatt tctgccggag aagcccacag catggcctta tccatgtctg gcaacattta
     1321 ttcatgggga aaaaatgaat gtggacaact aggcctgggc cacactgaga gtaaagatga
     1381 tccatccctt attgaaggac tagacaatca gaaagttgaa tttgtcgctt gtggtggctc
     1441 tcacagtgcc ctactcacac aggatgggct gctgtttact ttcggtgctg gaaaacatgg
     1501 gcaacttggt cataattcaa cacagaatga gctaagaccc tgtttggtgg ctgagcttgt
     1561 tgggtataga gtgactcaga tagcatgtgg aaggtggcac acacttgcct atgtttctga
     1621 tttgggaaag gtcttttcct ttggttctgg aaaagatgga caactgggaa atggtggaac
     1681 acgtgaccag ctgatgccgc ttccagtgaa agtatcatca agtgaagaac tcaaacttga
     1741 aagccatacc tcagaaaagg agttaataat gattgctgga gggaatcaaa gcattttgct
     1801 ctggataaag aaagagaatt catatgttaa tctgaagagg acaattccta ctctgaatga
     1861 agggactgta aagagatgga ttgctgatgt ggagactaaa cggtggcaga gcacaaaaag
     1921 ggaaatccaa gagatatttt catctcctgc ttgtctaact ggaagttttt taaggaaaag
     1981 aagaactaca gaaatgatgc ctgtttattt ggacttaaat aaagcaagaa acatcttcaa
     2041 ggagttaacc caaaaggact ggattactaa catgataacc acctgcctca aagataatct
     2101 gctcaaaaga cttccatttc attctccacc ccaagaagct ttagaaattt tcttccttct
     2161 cccagaatgt cctatgatgc atatttccaa caactgggag agccttgtgg ttccatttgc
     2221 aaaggttgtt tgtaaaatga gtgaccagtc ttcactggtt ctggaagagt attgggcaac
     2281 tctgcaagaa tccactttca gcaaactggt ccagatgttt aaaacagccg tcatatgcca
     2341 gttggattac tgggatgaaa gtgctgagga gaatggtaat gttcaagctc tcctagaaat
     2401 gttgaagaag ctgcacaggg taaaccaggt gaaatgtcaa ctacctgaaa gtattttcca
     2461 agtagacgaa ctcttgcacc gtctcaattt ttttgtagaa gtatgcagaa ggtacttgtg
     2521 gaaaatgact gtggacgctt cagaaaatgt acaatgctgc gtcatattca gtcactttcc
     2581 atttatcttt aataatctgt cgaaaattaa actactacat acagacacac ttttaaaaat
     2641 agagagtaaa aaacataaag cttatcttag gtcggcagca attgaggaag aaagagagtc
     2701 tgaattcgct ttgaggccca cgtttgatct aacagtcaga aggaatcact tgattgagga
     2761 tgttttgaat cagctaagtc aatttgagaa tgaagacctg aggaaagagt tatgggtttc
     2821 atttagtgga gaaattgggt atgacctcgg aggagtcaag aaagagttct tctactgtct
     2881 gtttgcagag atgatccagc cggaatatgg gatgttcatg tatcctgaag gggcttcctg
     2941 catgtggttt cctgtcaagc ctaaatttga gaagaaaaga tacttctttt ttggggttct
     3001 atgtggactt tccctgttca attgcaatgt tgccaacctt cctttcccac tggcactgtt
     3061 taagaaactt ttggaccaaa tgccatcatt ggaagacttg aaagaactca gtcctgattt
     3121 gggaaagaat ttgcaaacac ttctggatga tgaaggtgat aactttgagg aagtatttta
     3181 catccatttt aatgtgcact gggacagaaa cgacacaaac ttaattccta atggaagtag
     3241 cataactgtc aaccagacta acaagagaga ctatgtttct aagtatatca attacatttt
     3301 caacgactct gtaaaggcgg tttatgaaga atttcggaga ggattttata aaatgtgcga
     3361 cgaagacatt atcaaattat tccaccccga agaactgaag gatgtgattg ttggaaatac
     3421 agattatgat tggaaaacat ttgaaaagaa tgcacgttat gaaccaggat ataacagttc
     3481 acatcccacc atagtgatgt tttggaaggc tttccacaaa ttgactctgg aagaaaagaa
     3541 aaaattcctt gtatttctta caggaactga cagactacaa atgaaagatt taaataatat
     3601 gaaaataaca ttttgctgtc ctgaaagttg gaatgaaaga gaccctataa gagcactgac
     3661 atgtttcagt gtcctcttcc tccctaaata ttctacaatg gaaacagttg aagaagcgct
     3721 tcaagaagcc atcaacaaca acagaggatt tggcctcgag tacccatacg acgtacctga
     3781 ttatgcaact tatccttatg atgtacctga ttatgctagc tatccttatg atgtcccaga
     3841 ctacgcatag tgagggcccg atatctcgac aatcaacctc tggattacaa aatttgtgaa
     3901 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3961 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4021 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4081 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4141 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4201 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4261 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4321 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4381 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4441 ctttgggccg cctccccgcc tggaaacggg ggaggctaac tgaaacacgg aaggagacaa
     4501 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt
     4561 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     4621 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     4681 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagca
     4741 gatctgcgca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     4801 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4861 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4921 ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4981 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5041 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5101 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat
     5161 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     5221 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5281 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     5341 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5401 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5461 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     5521 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     5581 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     5641 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     5701 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     5761 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     5821 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     5881 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     5941 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     6001 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     6061 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     6121 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     6181 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     6241 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     6301 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     6361 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     6421 gttcgcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     6481 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6541 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     6601 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6661 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     6721 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6781 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6841 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6901 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6961 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7021 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7081 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7141 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7201 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7261 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7321 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     7381 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7441 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7501 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7561 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7621 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7681 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7741 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7801 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7861 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7921 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7981 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8041 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8101 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8161 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8221 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8281 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8341 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8401 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8461 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8521 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8581 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8641 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8701 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8761 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8821 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8881 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     8941 ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     9001 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     9061 gcttgaccga caattgcatg aagaatctgc ttagg
//
