LOCUS       V044988                 9694 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044988
VERSION     V044988
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..4350
                     /label="HDAC6(NM_006044)"
                     /note="HDAC6(NM_006044)"
                     /gene="HDAC6"
     CDS             4357..4446
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    4470..5058
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    5124..5172
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5374..5802
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5816..6145
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6212..7006
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7182..7303
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7352..7368)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7376..7392
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7400..7430)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7445..7466
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7754..8342)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8513..9373)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9374..9478)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgac ctcaaccggc
      721 caggattcca ccacaaccag gcagcgaaga agtaggcaga acccccagtc gccccctcag
      781 gactccagtg tcacttcgaa gcgaaatatt aaaaagggag ccgttccccg ctctatcccc
      841 aatctagcgg aggtaaagaa gaaaggcaaa atgaagaagc tcggccaagc aatggaagaa
      901 gacctaatcg tgggactgca agggatggat ctgaaccttg aggctgaagc actggctggc
      961 actggcttgg tgttggatga gcagttaaat gaattccatt gcctctggga tgacagcttc
     1021 ccggaaggcc ctgagcggct ccatgccatc aaggagcaac tgatccagga gggcctccta
     1081 gatcgctgcg tgtcctttca ggcccggttt gctgaaaagg aagagctgat gttggttcac
     1141 agcctagaat atattgatct gatggaaaca acccagtaca tgaatgaggg agaactccgt
     1201 gtcctagcag acacctacga ctcagtttat ctgcatccga actcatactc ctgtgcctgc
     1261 ctggcctcag gctctgtcct caggctggtg gatgcggtcc tgggggctga gatccggaat
     1321 ggcatggcca tcattaggcc tcctggacat cacgcccagc acagtcttat ggatggctat
     1381 tgcatgttca accacgtggc tgtggcagcc cgctatgctc aacagaaaca ccgcatccgg
     1441 agggtcctta tcgtagattg ggatgtgcac cacggtcaag gaacacagtt caccttcgac
     1501 caggacccca gtgtcctcta tttctccatc caccgctacg agcagggtag gttctggccc
     1561 cacctgaagg cctctaactg gtccaccaca ggtttcggcc aaggccaagg atataccatc
     1621 aatgtgcctt ggaaccaggt ggggatgcgg gatgctgact acattgctgc tttcctgcac
     1681 gtcctgctgc cagtcgccct cgagttccag cctcagctgg tcctggtggc tgctggattt
     1741 gatgccctgc aaggggaccc caagggtgag atggccgcca ctccggcagg gttcgcccag
     1801 ctaacccacc tgctcatggg tctggcagga ggcaagctga tcctgtctct ggagggtggc
     1861 tacaacctcc gcgccctggc tgaaggcgtc agtgcttcgc tccacaccct tctgggagac
     1921 ccttgcccca tgctggagtc acctggtgcc ccctgccgga gtgcccaggc ttcagtttcc
     1981 tgtgctctgg aagcccttga gcccttctgg gaggttcttg tgagatcaac tgagaccgtg
     2041 gagagggaca acatggagga ggacaatgta gaggagagcg aggaggaagg accctgggag
     2101 ccccctgtgc tcccaatcct gacatggcca gtgctacagt ctcgcacagg gctggtctat
     2161 gaccaaaata tgatgaatca ctgcaacttg tgggacagcc accaccctga ggtaccccag
     2221 cgcatcttgc ggatcatgtg ccgtctggag gagctgggcc ttgccgggcg ctgcctcacc
     2281 ctgacaccgc gccctgccac agaggctgag ctgctcacct gtcacagtgc tgagtacgtg
     2341 ggtcatctcc gggccacaga gaaaatgaaa acccgggagc tgcaccgtga gagttccaac
     2401 tttgactcca tctatatctg ccccagtacc ttcgcctgtg cacagcttgc cactggcgct
     2461 gcctgccgcc tggtggaggc tgtgctctca ggagaggttc tgaatggtgc tgctgtggtg
     2521 cgtcccccag gacaccacgc agagcaggat gcagcttgcg gtttttgctt tttcaactct
     2581 gtggctgtgg ctgctcgcca tgcccagact atcagtgggc atgccctacg gatcctgatt
     2641 gtggattggg atgtccacca cggtaatgga actcagcaca tgtttgagga tgaccccagt
     2701 gtgctatatg tgtccctgca ccgctatgat catggcacct tcttccccat gggggatgag
     2761 ggtgccagca gccagatcgg ccgggctgcg ggcacaggct tcaccgtcaa cgtggcatgg
     2821 aacgggcccc gcatgggtga tgctgactac ctagctgcct ggcatcgcct ggtgcttccc
     2881 attgcctacg agtttaaccc agaactggtg ctggtctcag ctggctttga tgctgcacgg
     2941 ggggatccgc tggggggctg ccaggtgtca cctgagggtt atgcccacct cacccacctg
     3001 ctgatgggcc ttgccagtgg ccgcattatc cttatcctag agggtggcta taacctgaca
     3061 tccatctcag agtccatggc tgcctgcact cgctccctcc ttggagaccc accacccctg
     3121 ctgaccctgc cacggccccc actatcaggg gccctggcct caatcactga gaccatccaa
     3181 gtccatcgca gatactggcg cagcttacgg gtcatgaagg tagaagacag agaaggaccc
     3241 tccagttcta agttggtcac caagaaggca ccccaaccag ccaaacctag gttagctgag
     3301 cggatgacca cacgagaaaa gaaggttctg gaagcaggca tggggaaagt cacctcggca
     3361 tcatttgggg aagagtccac tccaggccag actaactcag agacagctgt ggtggccctc
     3421 actcaggacc agccctcaga ggcagccaca gggggagcca ctctggccca gaccatttct
     3481 gaggcagcca ttgggggagc catgctgggc cagaccacct cagaggaggc tgtcggggga
     3541 gccactccgg accagaccac ctcagaggag actgtgggag gagccattct ggaccagacc
     3601 acctcagagg atgctgttgg gggagccacg ctgggccaga ctacctcaga ggaggctgta
     3661 ggaggagcta cactggccca gaccacctcg gaggcagcca tggagggagc cacactggac
     3721 cagactacgt cagaggaggc tccagggggc accgagctga tccaaactcc tctagcctcg
     3781 agcacagacc accagacccc cccaacctca cctgtgcagg gaactacacc ccagatatct
     3841 cccagtacac tgattgggag tctcaggacc ttggagctag gcagcgaatc tcagggggcc
     3901 tcagaatctc aggccccagg agaggagaac ctactaggag aggcagctgg aggtcaggac
     3961 atggctgatt cgatgctgat gcagggatct aggggcctca ctgatcaggc catattttat
     4021 gctgtgacac cactgccctg gtgtccccat ttggtggcag tatgccccat acctgcagca
     4081 ggcctagacg tgacccaacc ttgtggggac tgtggaacaa tccaagagaa ttgggtgtgt
     4141 ctctcttgct atcaggtcta ctgtggtcgt tacatcaatg gccacatgct ccaacaccat
     4201 ggaaattctg gacacccgct ggtcctcagc tacatcgacc tgtcagcctg gtgttactac
     4261 tgtcaggcct atgtccacca ccaggctctc ctagatgtga agaacatcgc ccaccagaac
     4321 aagtttgggg aggatatgcc ccacccacac ctcgaggaac aaaaactaat atcagaggaa
     4381 gatttggaac agaaactaat cagtgaagaa gatttagagc aaaagttaat ttccgaggag
     4441 gacttatagt gagggcccga tatctcgaca atcaacctct ggattacaaa atttgtgaaa
     4501 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4561 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4621 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4681 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4741 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4801 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4861 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4921 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4981 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5041 tttgggccgc ctccccgcct ggaaacgggg gaggctaact gaaacacgga aggagacaat
     5101 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
     5161 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     5221 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     5281 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag
     5341 atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     5401 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5461 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5521 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5581 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5641 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5701 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt
     5761 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     5821 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5881 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5941 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6001 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6061 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     6121 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     6181 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     6241 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     6301 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     6361 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     6421 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     6481 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     6541 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     6601 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6661 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6721 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6781 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6841 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6901 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6961 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     7021 ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     7081 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     7141 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     7201 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7261 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     7321 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7381 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7441 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7501 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7561 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7621 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7681 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7741 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7801 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7861 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7921 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     7981 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8041 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8101 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8161 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8221 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8281 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8341 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8401 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8461 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8521 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8581 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8641 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8701 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8761 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8821 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8881 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8941 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9001 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9061 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9121 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9181 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9241 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9301 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9361 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9421 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9481 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     9541 gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     9601 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     9661 cttgaccgac aattgcatga agaatctgct tagg
//
