LOCUS       V028970                 8032 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028970
VERSION     V028970
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2712
                     /label="HDAC10(NM_032019)"
                     /note="HDAC10(NM_032019)"
                     /gene="HDAC10"
     CDS             2719..2784
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    2808..3396
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3462..3510
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3712..4140
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4154..4483
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4550..5344
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5520..5641
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5690..5706)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5714..5730
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5738..5768)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5783..5804
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6092..6680)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6851..7711)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7712..7816)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggg gaccgcgctt
      721 gtgtaccatg aggacatgac ggccacccgg ctgctctggg acgaccccga gtgcgagatc
      781 gagcgtcctg agcgcctgac cgcagccctg gatcgcctgc ggcagcgcgg cctggaacag
      841 aggtgtctgc ggttgtcagc ccgcgaggcc tcggaagagg agctgggcct ggtgcacagc
      901 ccagagtatg tatccctggt cagggagacc caggtcctag gcaaggagga gctgcaggcg
      961 ctgtccggac agttcgacgc catctacttc cacccgagta cctttcactg cgcgcggctg
     1021 gccgcagggg ctggactgca gctggtggac gctgtgctca ctggagctgt gcaaaatggg
     1081 cttgccctgg tgaggcctcc cgggcaccat ggccagaggg cggctgccaa cgggttctgt
     1141 gtgttcaaca acgtggccat agcagctgca catgccaagc agaaacacgg gctacacagg
     1201 atcctcgtcg tggactggga tgtgcaccat ggccagggga tccagtatct ctttgaggat
     1261 gaccccagcg tcctttactt ctcctggcac cgctatgagc atgggcgctt ctggcctttc
     1321 ctgcgagagt cagatgcaga cgcagtgggg cggggacagg gcctcggctt cactgtcaac
     1381 ctgccctgga accaggttgg gatgggaaac gctgactacg tggctgcctt cctgcacctg
     1441 ctgctcccac tggcctttga gtttgaccct gagctggtgc tggtctcggc aggatttgac
     1501 tcagccatcg gggaccctga ggggcaaatg caggccacgc cagagtgctt cgcccacctc
     1561 acacagctgc tgcaggtgct ggccggcggc cgggtctgtg ccgtgctgga gggcggctac
     1621 cacctggagt cactggcgga gtcagtgtgc atgacagtac agacgctgct gggtgacccg
     1681 gccccacccc tgtcagggcc aatggcgcca tgtcagagtg ccctagagtc catccagagt
     1741 gcccgtgctg cccaggcccc gcactggaag agcctccagc agcaagatgt gaccgctgtg
     1801 ccgatgagcc ccagcagcca ctccccagag gggaggcctc cacctctgct gcctgggggt
     1861 ccagtgtgta aggcagctgc atctgcaccg agctccctcc tggaccagcc gtgcctctgc
     1921 cccgcaccct ctgtccgcac cgctgttgcc ctgacaacgc cggatatcac attggttctg
     1981 ccccctgacg tcatccaaca ggaagcgtca gccctgaggg aggagacaga agcctgggcc
     2041 aggccacacg agtccctggc ccgggaggag gccctcactg cacttgggaa gctcctgtac
     2101 ctcttagatg ggatgctgga tgggcaggtg aacagtggta tagcagccac tccagcctct
     2161 gctgcagcag ccaccctgga tgtggctgtt cggagaggcc tgtcccacgg agcccagagg
     2221 ctgctgtgcg tggccctggg acagctggac cggcctccag acctcgccca tgacgggagg
     2281 agtctgtggc tgaacatcag gggcaaggag gcggctgccc tatccatgtt ccatgtctcc
     2341 acgccactgc cagtgatgac cggtggtttc ctgagctgca tcttgggctt ggtgctgccc
     2401 ctggcctatg gcttccagcc tgacctggtg ctggtggcgc tggggcctgg ccatggcctg
     2461 cagggccccc acgctgcact cctggctgca atgcttcggg ggctggcagg gggccgagtc
     2521 ctggccctcc tggaggagaa ctccacaccc cagctagcag ggatcctggc ccgggtgctg
     2581 aatggagagg cacctcctag cctaggccct tcctctgtgg cctccccaga ggacgtccag
     2641 gccctgatgt acctgagagg gcagctggag cctcagtgga agatgttgca gtgccatcct
     2701 cacctggtgg ctctcgagga ctacaaagac catgacggtg attataaaga tcatgacatc
     2761 gactacaagg atgacgatga caagtagtga gggcccgata tctcgacaat caacctctgg
     2821 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2881 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2941 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3001 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3061 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3121 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3181 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     3241 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3301 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3361 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aaacggggga ggctaactga
     3421 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     3481 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     3541 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     3601 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     3661 caggccctgc catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct
     3721 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3781 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3841 gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac
     3901 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3961 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4021 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4081 tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4141 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4201 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4261 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4321 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4381 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     4441 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     4501 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4561 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4621 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     4681 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     4741 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     4801 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     4861 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     4921 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     4981 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5041 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     5101 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5161 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5221 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5281 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5341 ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     5401 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5461 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     5521 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5581 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5641 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     5701 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5761 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     5821 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     5881 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5941 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6001 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6061 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6121 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6181 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6241 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     6301 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6361 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6421 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6481 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     6541 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6601 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6661 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6721 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6781 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6841 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6901 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6961 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7021 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7081 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7141 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7201 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7261 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7321 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7381 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7441 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7501 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7561 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7621 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7681 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7741 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7801 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     7861 atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag
     7921 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     7981 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gg
//
