LOCUS V028970 8032 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028970 VERSION V028970 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..2712 /label="HDAC10(NM_032019)" /note="HDAC10(NM_032019)" /gene="HDAC10" CDS 2719..2784 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 2808..3396 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3462..3510 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3712..4140 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4154..4483 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4550..5344 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5520..5641 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5690..5706) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5714..5730 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5738..5768) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5783..5804 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6092..6680) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6851..7711) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7712..7816) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggg gaccgcgctt 721 gtgtaccatg aggacatgac ggccacccgg ctgctctggg acgaccccga gtgcgagatc 781 gagcgtcctg agcgcctgac cgcagccctg gatcgcctgc ggcagcgcgg cctggaacag 841 aggtgtctgc ggttgtcagc ccgcgaggcc tcggaagagg agctgggcct ggtgcacagc 901 ccagagtatg tatccctggt cagggagacc caggtcctag gcaaggagga gctgcaggcg 961 ctgtccggac agttcgacgc catctacttc cacccgagta cctttcactg cgcgcggctg 1021 gccgcagggg ctggactgca gctggtggac gctgtgctca ctggagctgt gcaaaatggg 1081 cttgccctgg tgaggcctcc cgggcaccat ggccagaggg cggctgccaa cgggttctgt 1141 gtgttcaaca acgtggccat agcagctgca catgccaagc agaaacacgg gctacacagg 1201 atcctcgtcg tggactggga tgtgcaccat ggccagggga tccagtatct ctttgaggat 1261 gaccccagcg tcctttactt ctcctggcac cgctatgagc atgggcgctt ctggcctttc 1321 ctgcgagagt cagatgcaga cgcagtgggg cggggacagg gcctcggctt cactgtcaac 1381 ctgccctgga accaggttgg gatgggaaac gctgactacg tggctgcctt cctgcacctg 1441 ctgctcccac tggcctttga gtttgaccct gagctggtgc tggtctcggc aggatttgac 1501 tcagccatcg gggaccctga ggggcaaatg caggccacgc cagagtgctt cgcccacctc 1561 acacagctgc tgcaggtgct ggccggcggc cgggtctgtg ccgtgctgga gggcggctac 1621 cacctggagt cactggcgga gtcagtgtgc atgacagtac agacgctgct gggtgacccg 1681 gccccacccc tgtcagggcc aatggcgcca tgtcagagtg ccctagagtc catccagagt 1741 gcccgtgctg cccaggcccc gcactggaag agcctccagc agcaagatgt gaccgctgtg 1801 ccgatgagcc ccagcagcca ctccccagag gggaggcctc cacctctgct gcctgggggt 1861 ccagtgtgta aggcagctgc atctgcaccg agctccctcc tggaccagcc gtgcctctgc 1921 cccgcaccct ctgtccgcac cgctgttgcc ctgacaacgc cggatatcac attggttctg 1981 ccccctgacg tcatccaaca ggaagcgtca gccctgaggg aggagacaga agcctgggcc 2041 aggccacacg agtccctggc ccgggaggag gccctcactg cacttgggaa gctcctgtac 2101 ctcttagatg ggatgctgga tgggcaggtg aacagtggta tagcagccac tccagcctct 2161 gctgcagcag ccaccctgga tgtggctgtt cggagaggcc tgtcccacgg agcccagagg 2221 ctgctgtgcg tggccctggg acagctggac cggcctccag acctcgccca tgacgggagg 2281 agtctgtggc tgaacatcag gggcaaggag gcggctgccc tatccatgtt ccatgtctcc 2341 acgccactgc cagtgatgac cggtggtttc ctgagctgca tcttgggctt ggtgctgccc 2401 ctggcctatg gcttccagcc tgacctggtg ctggtggcgc tggggcctgg ccatggcctg 2461 cagggccccc acgctgcact cctggctgca atgcttcggg ggctggcagg gggccgagtc 2521 ctggccctcc tggaggagaa ctccacaccc cagctagcag ggatcctggc ccgggtgctg 2581 aatggagagg cacctcctag cctaggccct tcctctgtgg cctccccaga ggacgtccag 2641 gccctgatgt acctgagagg gcagctggag cctcagtgga agatgttgca gtgccatcct 2701 cacctggtgg ctctcgagga ctacaaagac catgacggtg attataaaga tcatgacatc 2761 gactacaagg atgacgatga caagtagtga gggcccgata tctcgacaat caacctctgg 2821 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 2881 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 2941 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 3001 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 3061 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 3121 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 3181 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca 3241 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 3301 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc 3361 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aaacggggga ggctaactga 3421 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa 3481 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc 3541 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt 3601 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg 3661 caggccctgc catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct 3721 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 3781 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 3841 gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac 3901 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 3961 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 4021 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 4081 tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 4141 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 4201 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 4261 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 4321 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 4381 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 4441 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 4501 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 4561 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 4621 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 4681 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 4741 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 4801 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 4861 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 4921 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 4981 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 5041 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 5101 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 5161 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 5221 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 5281 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 5341 ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac 5401 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 5461 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 5521 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 5581 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 5641 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 5701 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 5761 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 5821 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 5881 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 5941 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 6001 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 6061 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 6121 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 6181 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 6241 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta 6301 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 6361 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 6421 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 6481 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 6541 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 6601 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 6661 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 6721 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 6781 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 6841 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 6901 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 6961 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 7021 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 7081 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 7141 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 7201 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 7261 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 7321 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 7381 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 7441 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 7501 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 7561 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 7621 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 7681 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 7741 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 7801 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 7861 atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag 7921 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta 7981 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gg //