LOCUS       62056_9290        7744 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        202..581
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             893..919
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     polyA_signal    3311..3535
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3581..4009
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4023..4352
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4419..5210
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5387..5520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5557..5573)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5581..5597)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5605..5635)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5650..5671)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5959..6544)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6718..7575)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7576..7680)
                     /label=AmpR promoter
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttgccacca tgtaccctta
      901 tgacgtcccc gattacgccg gtaccgagct cggatcccac aaatcaagag ttaaaggtta
      961 tctgagacta aaaatgactt atttacctaa aaccagtggc tcagaagatg ataatgcaga
     1021 acaggctgag gaattagagc ctggctgggt tgttttggac caaccagatg ctgcttgcca
     1081 tttgcagcaa caacaagaac cttctcctct acctccaggg tgggaagaga ggcaggatat
     1141 ccttggaagg acctattatg taaaccatga atctagaaga acacagtgga aaagaccaac
     1201 ccctcaggac aacctaacag atgctgagaa tggcaacatt caactgcaag cacaacgtgc
     1261 atttaccacc aggcggcaga tatccgagga aacagaaagt gttgacaacc gagagtcttc
     1321 cgagaactgg gaaattataa gagaagatga agccaccatg tatagcaacc aggccttccc
     1381 atcacctcca ccgtcaagta acttggatgt tccaactcat cttgcagaag aattgaatgc
     1441 cagactcacc atttttggaa attcagccgt gagccagcca gcatcgagct caaatcattc
     1501 cagcagaaga ggcagcttac aagcctatac ttttgaggaa caacctacac ttcctgtgct
     1561 tttgcctact tcatctggat taccaccagg ttgggaagaa aaacaagatg aaagaggaag
     1621 atcatattat gtagatcaca attccagaac gactacttgg acaaagccca ctgtacaggc
     1681 cacagtggag accagtcagc tgacctcaag ccagagttct gcaggccctc aatcacaagc
     1741 ctccaccagt gattcaggcc agcaggtgac ccagccatct gaaattgagc aaggattcct
     1801 tcctaaaggc tgggaagtcc ggcatgcacc aaatgggagg cctttcttta ttgaccacaa
     1861 cactaaaacc accacctggg aagatccaag attgaaaatt ccagcccatc tgagaggaaa
     1921 gacatcactt gatacttcca atgatctagg gcctttacct ccaggatggg aagagagaac
     1981 tcacacagat ggaagaatct tctacataaa tcacaatata aaaagaacac aatgggaaga
     2041 tcctcggttg gagaatgtag caataactgg accagcagtg ccctactcca gggattacaa
     2101 aagaaagtat gagttcttcc gaagaaagtt gaagaagcag aatgacattc caaacaaatt
     2161 tgaaatgaaa cttcgccgag caactgttct tgaagactct taccggagaa ttatgggtgt
     2221 caagagagca gacttcctga aggctcgact gtggattgag tttgatggtg aaaagggatt
     2281 ggattatgga ggagttgcca gagaatggtt cttcctgatc tcaaaggaaa tgtttaaccc
     2341 ttattatggg ttgtttgaat attctgctac ggacaattat accctacaga taaatccaaa
     2401 ctctggattg tgtaacgaag atcacctctc ttacttcaag tttattggtc gggtagctgg
     2461 aatggcagtt tatcatggca aactgttgga tggttttttc atccgcccat tttacaagat
     2521 gatgcttcac aaaccaataa cccttcatga tatggaatct gtggatagtg aatattacaa
     2581 ttccctaaga tggattcttg aaaatgaccc aacagaattg gacctcaggt ttatcataga
     2641 tgaagaactt tttggacaga cacatcaaca tgagctgaaa aatggtggat cagaaatagt
     2701 tgtcaccaat aagaacaaaa aggaatatat ttatcttgta atacaatggc gatttgtaaa
     2761 ccgaatccag aagcaaatgg ctgcttttaa agagggattc tttgaactaa taccacagga
     2821 tctcatcaaa atttttgatg aaaatgaact agagcttctt atgtgtggac tgggagatgt
     2881 tgatgtgaat gactggaggg aacatacaaa gtataaaaat ggctacagtg caaatcatca
     2941 ggttatacag tggttttgga aggctgtttt aatgatggat tcagaaaaaa gaataagatt
     3001 acttcagttt gtcactggca catctcgggt gcctatgaat ggatttgctg aactatacgg
     3061 ttcaaatgga ccacagtcat ttacagttga acagtggggt actcctgaaa agctgccaag
     3121 agctcatacc tgttttaatc gcctggactt gccaccttat gaatcatttg aagaattatg
     3181 ggataaactt cagatggcaa ttgaaaacac ccagggcttt gatggagttg attagaattc
     3241 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga
     3301 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     3361 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     3421 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     3481 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     3541 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     3601 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3661 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3721 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3781 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3841 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3901 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3961 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     4021 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4081 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4141 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4201 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4261 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     4321 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     4381 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     4441 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4501 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4561 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     4621 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4681 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4741 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     4801 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     4861 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     4921 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     4981 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5041 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5101 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     5161 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5221 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     5281 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     5341 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     5401 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5461 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     5521 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5581 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5641 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5701 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5761 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5821 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5881 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5941 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6001 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6061 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6121 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6181 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6241 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6301 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6361 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6421 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6481 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6541 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6601 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     6661 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6721 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6781 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6841 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6901 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6961 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7021 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7081 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7141 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7201 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7261 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7321 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7381 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7441 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7501 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7561 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7621 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7681 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     7741 tggt
//
