LOCUS V025326 6424 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025326 VERSION V025326 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..1083 /label="H2BC21(NM_003528)" /note="H2BC21(NM_003528)" /gene="H2BC21" CDS 1090..1116 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1120..1146 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1150..1176 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 1200..1788 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 1854..1902 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2104..2532 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2546..2875 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2942..3736 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3912..4033 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4082..4098) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4106..4122 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4130..4160) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4175..4196 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4484..5072) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5243..6103) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6104..6208) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgcc tgaaccggca 721 aaatccgctc cggcccctaa aaagggctcc aagaaagccg tcaccaaagc ccagaagaaa 781 gacggcaaga agcgcaagcg cagccgcaaa gagagctact ccatctacgt gtacaaggtg 841 ctgaagcagg tccaccccga caccggcatc tcgtccaagg ccatgggcat catgaactcc 901 ttcgtcaacg acatcttcga gcgcatcgcg ggagaggctt cccgcctggc gcactacaac 961 aagcgctcca ccatcacatc ccgcgagatc cagacggccg tgcgcctgct gctgcccggc 1021 gagctggcca agcacgccgt gtccgagggc accaaggcgg tcaccaagta caccagctcc 1081 aagctcgagt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat 1141 tatgctagct atccttatga tgtcccagac tacgcatagt gagggcccga tatctcgaca 1201 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 1261 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 1321 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 1381 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 1441 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 1501 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 1561 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 1621 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 1681 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 1741 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaaacgggg 1801 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat 1861 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt 1921 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg 1981 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca 2041 acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc 2101 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 2161 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 2221 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat 2281 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 2341 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 2401 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 2461 tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 2521 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 2581 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 2641 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 2701 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 2761 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 2821 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 2881 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 2941 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 3001 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 3061 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 3121 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 3181 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 3241 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 3301 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 3361 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 3421 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 3481 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 3541 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 3601 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 3661 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 3721 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca 3781 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 3841 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 3901 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 3961 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 4021 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 4081 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 4141 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 4201 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 4261 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 4321 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 4381 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 4441 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 4501 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 4561 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 4621 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat 4681 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 4741 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 4801 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 4861 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 4921 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 4981 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 5041 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 5101 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 5161 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 5221 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 5281 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 5341 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 5401 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 5461 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 5521 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 5581 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 5641 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 5701 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 5761 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 5821 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 5881 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 5941 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 6001 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 6061 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 6121 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 6181 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 6241 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc 6301 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc 6361 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct 6421 tagg //