LOCUS       V030896                 6417 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030896
VERSION     V030896
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2564
                     /label="Glp2r(NM_175681)"
                     /note="Glp2r(NM_175681)"
                     /gene="Glp2r"
     CDS             2583..2612
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2631..2654
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2674..2690)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2701..3323
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3472..4060)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4389..4436
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4668..5462)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5497..5854)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5856..5960)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5987..6415
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gcgtcggctc tggggccctg ggacgccctt cctcgccctg cttctgctgg
     1081 tttccatcaa gcaagttact acaggatctc tccttgagga gacagttcag aagtgggctc
     1141 agtataagga gacgtgtctg aaagacttgc tcgagaaacc ttctggtgta ttttgtaatg
     1201 gaacatttga taagtacgtg tgctggcctc attcttttcc tggaaatgtc tctgtcccct
     1261 gtccttcata cttaccttgg tggaataaag agagcccagg aagggcctac agacactgct
     1321 tggctcaggg gacttggcag aagcaagaga actccacaga tacttggcag gatgagtcag
     1381 aatgctcaga gaaccacagc ttcaaacaaa acgtggatca ctatcaccat accttgttgt
     1441 ccaccttgca gctgatgtac actgtgggct actccctgtc tctcatctcc ctcttcttgg
     1501 ctcttacact cttcttgttc cttcgaaaac tgcactgcac acgcaattac atccacatga
     1561 acctgttcgc ttcgttcatc ctgagagctc tggttgtcct tgtgaaggac atggtcttct
     1621 acaactctta ctccaggagg cctgacagtg agagtggatg gatgtcatat ctgtcagaga
     1681 tttctgcctc ctgccgctct gtccaggtcc tcctgcacta ctttgtgggc accaatcact
     1741 tgtggttgct ggttgaagga ctttacctcc atgctctgct ggagccaaca gtgcttcctg
     1801 aaaggcggct gtggcccaag tacctggtgg tgggttgggc cttccccatg ctgtttgtta
     1861 ttccctggat ttttgtccga gcaagcctgg agaatacagg gtgctgggca gtaaatgaga
     1921 ataagaaaat ctggtggatc atcagaggac ccattctgct ttgtgtaaca gttaatttct
     1981 tcatcttcct caagattctc aagcttctca tttctaagtt cagagctcat cagatgtgct
     2041 tcagagacta caaatataga ctggcaaaat caaccctgct cctcattctt ttgatggggg
     2101 ttcatgagtt cctcttcact ttcttcaccg acgaccaagt tcaaggattt tcaagactta
     2161 ttcgtctctt catccagttg accctgagct ccttccatgg atttctggtg gccttgcagt
     2221 acggctttgc cagtagagag gtgaaggcag agctgagaaa gacctggggc cgcttcttat
     2281 tagctcgcca ctggggctgc agagcctgtg tcctagggaa gaacttccgg ttcctgggga
     2341 aatgttccaa gaagctgtcg gagggagatg gtgccgagac actccagaag ctgcaatctt
     2401 caggtgtcag ctcacacctg accgctggga acctgcggga tcatggggca cagcctcaca
     2461 ggggccgtgg agcttggccc cgggcaagca gcctgtctga gagcagcgag ggagacttca
     2521 ccctggccaa taccatggag gagattctgg aagagagtga gatcacgcgt acgcggccgc
     2581 tcgagcagaa actcatctca gaagaggatc tggcagcaaa tgatatcctg gattacaagg
     2641 atgacgacga taaggtttaa acggccggcc gcggtcatag ctgtttcctg aacagatccc
     2701 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     2761 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     2821 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     2881 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     2941 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     3001 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     3061 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     3121 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     3181 attttaaaat aactatacca gcaggaggac gtccagacac agcataggct acctggccat
     3241 gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc
     3301 actcagtaga tgcctgttga attgggtacg cggccagcgg cgagcggtat cagctcactc
     3361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3901 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4201 aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag
     4261 ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta
     4321 ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc
     4381 gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat
     4441 agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg
     4501 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa
     4561 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg
     4621 caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg
     4681 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     4741 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     4801 atgtcctgat agcgatccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     4861 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
     4921 ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     4981 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     5041 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     5101 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     5161 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     5221 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     5281 tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     5341 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     5401 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     5461 atgcgaaacg atcctcatcc tgtctcttga tcgatctttg caaaagccta ggcctccaaa
     5521 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     5581 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     5641 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     5701 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     5761 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     5821 acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca atattattga
     5881 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5941 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
     6001 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6061 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6121 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6181 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6241 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6301 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6361 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaatttt
//
