LOCUS       V031032                 6663 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031032
VERSION     V031032
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2810
                     /label="Gatad2b(NM_001379655)"
                     /note="Gatad2b(NM_001379655)"
                     /gene="Gatad2b"
     CDS             2829..2858
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2877..2900
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2920..2936)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2947..3569
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3718..4306)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4635..4682
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4914..5708)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5743..6100)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6102..6206)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6233..6661
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggatagaatg acagaagatg ctctgcgcct gaatcttttg aagaggagtt
     1081 tggacccagc agatgagcga gatgatgttc tggcaaaacg actgaaaatg gaggggcatg
     1141 aggccatgga acggctgaaa atgttagcac tgctcaaaag gaaagatttg gcaaatcttg
     1201 aggtgccgca tgagttacct actaagcaag atggcagtgg tgtcaagggt tatgaagaga
     1261 aacttaatgg aaatctcagg cctcatggag acaacaacag gactgctgga aggcccggca
     1321 aagaaaacat caatgatgag cctgtggata tgagtgctag acggagtgag ccagaccgag
     1381 gaaggctaac tccttcccca gacataattg ttttgtctga taatgaggcc tccagtcccc
     1441 gatccagctc ccggatggaa gagagactca aagcagccaa cctagagatg tttaagggga
     1501 aaggcatgga ggagcggcag cagcttatca agcaactgag ggacgagcta cgattggaag
     1561 aagctcgcct ggtgctgcta aagaaactga gacagagtca gctacagaaa gagaatgtgg
     1621 tccaaaagac tccagttgta cagaatgcag catctattgt tcagccatct cctgcacatg
     1681 tgggacaaca aggcttatct aagcttccct cccgacccgg ggctcaagga attgaacctc
     1741 aaaatatgag aacattacag ggtcacagtg tcatccgttc agcaaccaac accacccttc
     1801 cacacatgtt gatgtctcaa cgtgttattg caccaaatcc agcccaactt cagggccagc
     1861 ggggtccacc gaagcctggc attgtacgca ccacaacacc taacatgaat cctgccatca
     1921 gctaccaacc acagtcaagt tcgtctgttc cttgtcagcg cacaacgtct tctgccatct
     1981 atatgaacct tgcctcccat attcaaccag ggactgtgaa cagagtgtcc tcaccacttc
     2041 ctagccccag tgccatgagt gacgctgcca actcacaggc tgcagccaag ttggcccttc
     2101 gaaaacagct ggaaaagacg ctcctggaga taccacctcc aaagcctcct gctcccttgc
     2161 ttcacttcct gcctagtgca gccaatagcg agtttatcta catggtaggc ttggaagaag
     2221 ttgtacagag tgtcattgac agccaaggca aaaactgtgc ctcacttctg agggtcgaac
     2281 cctttgtatg tgcccagtgc cgcacagatt tcacccctca ctggaagcaa gaaaagaatg
     2341 gtaagatcct ctgtgagcag tgtatgactt ccaaccagaa aaaggctcta aaagctgaac
     2401 acactaaccg gctgaaaaac gctttcgtta aagccctaca gcaggaacag gaaattgaac
     2461 agcgattaca gcagcaggca gcactctccc caacgacagc tccagctgta tccagtgtca
     2521 gtaaacagga gaccatcatg agacatcata cactgcggca ggctccacag ccccagagca
     2581 gcctccagcg tggtataccc acatctgccc gatccatgct ttcaaacttt gcacaggcac
     2641 cccaattgtc tgtgccaggt ggcctccttg gtatgccagg tgtcaacatt gcatacttga
     2701 acactggcat cggaggacat aaagccccaa gtttggcaga ccggcagcgt gaataccttt
     2761 tagacatgat ccctcccagg tctatatcgc agtccatcag tgggcagaaa acgcgtacgc
     2821 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt
     2881 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca
     2941 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     3001 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     3061 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     3121 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     3181 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     3241 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     3301 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     3361 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     3421 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct
     3481 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt
     3541 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc
     3601 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3661 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3721 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3781 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3841 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3901 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3961 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4021 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4081 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4141 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4201 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4261 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4321 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4381 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4441 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc
     4501 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg
     4561 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg
     4621 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc
     4681 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct
     4741 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     4801 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     4861 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
     4921 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     4981 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     5041 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa
     5101 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     5161 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     5221 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     5281 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     5341 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     5401 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     5461 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     5521 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     5581 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     5641 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     5701 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc
     5761 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     5821 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     5881 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     5941 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     6001 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     6061 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat
     6121 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6181 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     6241 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6301 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6361 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6421 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6481 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6541 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6601 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6661 ttt
//
