LOCUS V031032 6663 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031032 VERSION V031032 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..2810 /label="Gatad2b(NM_001379655)" /note="Gatad2b(NM_001379655)" /gene="Gatad2b" CDS 2829..2858 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2877..2900 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2920..2936) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2947..3569 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(3718..4306) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 4635..4682 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4914..5708) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(5743..6100) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(6102..6206) /gene="bla" /label="AmpR promoter" rep_origin 6233..6661 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat ggatagaatg acagaagatg ctctgcgcct gaatcttttg aagaggagtt 1081 tggacccagc agatgagcga gatgatgttc tggcaaaacg actgaaaatg gaggggcatg 1141 aggccatgga acggctgaaa atgttagcac tgctcaaaag gaaagatttg gcaaatcttg 1201 aggtgccgca tgagttacct actaagcaag atggcagtgg tgtcaagggt tatgaagaga 1261 aacttaatgg aaatctcagg cctcatggag acaacaacag gactgctgga aggcccggca 1321 aagaaaacat caatgatgag cctgtggata tgagtgctag acggagtgag ccagaccgag 1381 gaaggctaac tccttcccca gacataattg ttttgtctga taatgaggcc tccagtcccc 1441 gatccagctc ccggatggaa gagagactca aagcagccaa cctagagatg tttaagggga 1501 aaggcatgga ggagcggcag cagcttatca agcaactgag ggacgagcta cgattggaag 1561 aagctcgcct ggtgctgcta aagaaactga gacagagtca gctacagaaa gagaatgtgg 1621 tccaaaagac tccagttgta cagaatgcag catctattgt tcagccatct cctgcacatg 1681 tgggacaaca aggcttatct aagcttccct cccgacccgg ggctcaagga attgaacctc 1741 aaaatatgag aacattacag ggtcacagtg tcatccgttc agcaaccaac accacccttc 1801 cacacatgtt gatgtctcaa cgtgttattg caccaaatcc agcccaactt cagggccagc 1861 ggggtccacc gaagcctggc attgtacgca ccacaacacc taacatgaat cctgccatca 1921 gctaccaacc acagtcaagt tcgtctgttc cttgtcagcg cacaacgtct tctgccatct 1981 atatgaacct tgcctcccat attcaaccag ggactgtgaa cagagtgtcc tcaccacttc 2041 ctagccccag tgccatgagt gacgctgcca actcacaggc tgcagccaag ttggcccttc 2101 gaaaacagct ggaaaagacg ctcctggaga taccacctcc aaagcctcct gctcccttgc 2161 ttcacttcct gcctagtgca gccaatagcg agtttatcta catggtaggc ttggaagaag 2221 ttgtacagag tgtcattgac agccaaggca aaaactgtgc ctcacttctg agggtcgaac 2281 cctttgtatg tgcccagtgc cgcacagatt tcacccctca ctggaagcaa gaaaagaatg 2341 gtaagatcct ctgtgagcag tgtatgactt ccaaccagaa aaaggctcta aaagctgaac 2401 acactaaccg gctgaaaaac gctttcgtta aagccctaca gcaggaacag gaaattgaac 2461 agcgattaca gcagcaggca gcactctccc caacgacagc tccagctgta tccagtgtca 2521 gtaaacagga gaccatcatg agacatcata cactgcggca ggctccacag ccccagagca 2581 gcctccagcg tggtataccc acatctgccc gatccatgct ttcaaacttt gcacaggcac 2641 cccaattgtc tgtgccaggt ggcctccttg gtatgccagg tgtcaacatt gcatacttga 2701 acactggcat cggaggacat aaagccccaa gtttggcaga ccggcagcgt gaataccttt 2761 tagacatgat ccctcccagg tctatatcgc agtccatcag tgggcagaaa acgcgtacgc 2821 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt 2881 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca 2941 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc 3001 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag 3061 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg 3121 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa 3181 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc 3241 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag 3301 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac 3361 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc 3421 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct 3481 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt 3541 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc 3601 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 3661 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 3721 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 3781 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 3841 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 3901 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 3961 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 4021 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 4081 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 4141 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 4201 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 4261 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 4321 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 4381 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 4441 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc 4501 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg 4561 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg 4621 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc 4681 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct 4741 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc 4801 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg 4861 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag 4921 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa 4981 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc 5041 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa 5101 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga 5161 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc 5221 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct 5281 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc 5341 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac 5401 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca 5461 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc 5521 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc 5581 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag 5641 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt 5701 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc 5761 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc 5821 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt 5881 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat 5941 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta 6001 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc 6061 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat 6121 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 6181 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc 6241 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 6301 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 6361 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 6421 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 6481 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 6541 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 6601 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 6661 ttt //