LOCUS       V030903                 6246 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030903
VERSION     V030903
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2393
                     /label="Gabra1(NM_010250)"
                     /note="Gabra1(NM_010250)"
                     /gene="Gabra1"
     CDS             2412..2441
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2460..2483
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2503..2519)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2530..3152
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3301..3889)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4218..4265
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4497..5291)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5326..5683)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5685..5789)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5816..6244
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaagaaaagt cggggtctct ctgactatct ttgggcctgg accctcattc
     1081 tgagcacact gtcgggaaga agctatggac agccctccca agatgaactt aaagacaaca
     1141 ccactgtctt cacaagaatt ttggaccgac tgctggacgg ttatgacaat cgtctgagac
     1201 caggtttggg agagcgtgta actgaagtga agaccgacat tttcgtcacc agtttcggac
     1261 cagtttcaga ccacgatatg gagtatacaa tagatgtgtt tttccgtcaa agttggaagg
     1321 atgaaagatt aaaattcaaa ggacccatga cagtgctccg gctaaacaac cttatggcca
     1381 gtaaaatctg gactccagat acatttttcc acaatggaaa gaagtctgtg gcccacaaca
     1441 tgaccatgcc taataagctc ctgcgtatca cagaggatgg cactctgctg tacaccatga
     1501 ggttgaccgt gagagctgaa tgcccaatgc acttggaaga cttccctatg gatgcccatg
     1561 cctgcccact aaaattcgga agctatgctt atacaagagc agaagttgtc tatgagtgga
     1621 ccagagagcc agcccgttca gtggttgtag cagaagatgg gtcacgttta aaccagtatg
     1681 accttcttgg acaaacagtt gactctggaa ttgttcagtc cagtactgga gaatatgtgg
     1741 ttatgacgac tcacttccac ttgaagagaa aaattggcta ctttgttatt caaacatatc
     1801 tgccgtgcat aatgacagtt attctctccc aagtctcctt ctggctcaac agagagtcag
     1861 taccagcaag aactgtcttt ggagtgacga ctgttctgac tatgacaacc ttgagtatca
     1921 gtgccagaaa ttccctcccg aaggtggctt atgcaacagc tatggactgg tttattgcag
     1981 tatgctatgc ctttgttttc tcagctctga ttgagtttgc cacagtaaac tatttcacca
     2041 agagagggta tgcgtgggat ggcaaaagcg tggttccaga aaagccaaag aaagtaaagg
     2101 atcctctcat taagaaaaac aacacatatg ctcctacagc aaccagctat acccctaact
     2161 tagccagggg tgaccccggc ttggcaacta ttgctaaaag tgcgaccata gaaccgaaag
     2221 aagtcaagcc tgagacaaaa ccaccagaac ccaagaaaac ctttaacagc gtcagcaaaa
     2281 tcgaccgact gtcaagaata gcctttccgc tgctatttgg aatctttaac ttagtctatt
     2341 gggccacgta tttaaacaga gagcctcagc taaaagcccc cacaccccat caaacgcgta
     2401 cgcggccgct cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg
     2461 attacaagga tgacgacgat aaggtttaaa cggccggccg cggtcatagc tgtttcctga
     2521 acagatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     2581 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     2641 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     2701 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     2761 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     2821 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     2881 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     2941 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     3001 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta
     3061 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc
     3121 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc
     3181 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3721 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3841 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3901 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3961 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4021 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt
     4081 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac
     4141 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga
     4201 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat
     4261 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc
     4321 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     4381 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     4441 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     4501 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     4561 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     4621 gccaacgcta tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca
     4681 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     4741 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc
     4801 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     4861 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     4921 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     4981 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     5041 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     5101 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     5161 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     5221 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     5281 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag
     5341 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     5401 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     5461 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     5521 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     5581 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     5641 ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa
     5701 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5761 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     5821 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5881 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5941 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6001 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6061 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6121 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6181 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6241 aatttt
//
