LOCUS       62056_9280        6830 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        202..581
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             872..1525
                     /codon_start=1
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     polyA_signal    2397..2621
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2667..3095
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3109..3438
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3505..4296
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4473..4606
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4643..4659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4667..4683)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4691..4721)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4736..4757)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5045..5630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5804..6661)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6662..6766)
                     /label=AmpR promoter
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagccac catgtcccct atactaggtt attggaaaat
      901 taagggcctt gtgcaaccca ctcgacttct tttggaatat cttgaagaaa aatatgaaga
      961 gcatttgtat gagcgcgatg aaggtgataa atggcgaaac aaaaagtttg aattgggttt
     1021 ggagtttccc aatcttcctt attatattga tggtgatgtt aaattaacac agtctatggc
     1081 catcatacgt tatatagctg acaagcacaa catgttgggt ggttgtccaa aagagcgtgc
     1141 agagatttca atgcttgaag gagcggtttt ggatattaga tacggtgttt cgagaattgc
     1201 atatagtaaa gactttgaaa ctctcaaagt tgattttctt agcaagctac ctgaaatgct
     1261 gaaaatgttc gaagatcgtt tatgtcataa aacatattta aatggtgatc atgtaaccca
     1321 tcctgacttc atgttgtatg acgctcttga tgttgtttta tacatggacc caatgtgcct
     1381 ggatgcgttc ccaaaattag tttgttttaa aaaacgtatt gaagctatcc cacaaattga
     1441 taagtacttg aaatccagca agtatatagc atggcctttg cagggctggc aagccacgtt
     1501 tggtggtggc gaccatcctc caaaaggagg agggggaagc aagcttttca aaaagatcaa
     1561 agtgctgggc tccggtgcgt tcggcacggt gtataaggga ctctggatcc cagaaggtga
     1621 gaaagttaaa attcccgtcg ctatcaagga attaagagaa gcaacatctc cgaaagccaa
     1681 caaggaaatc ctcgatgaag cctacgtgat ggccagcgtg gacaaccccc acgtgtgccg
     1741 cctgctgggc atctgcctca cctccaccgt gcagctcatc acgcagctca tgcccttcgg
     1801 ctgcctcctg gactatgtcc gggaacacaa agacaatatt ggctcccagt acctgctcaa
     1861 ctggtgtgtg cagatcgcaa agggcatgaa ctacttggag gaccgtcgct tggtgcaccg
     1921 cgacctggca gccaggaacg tactggtgaa aacaccgcag catgtcaaga tcacagattt
     1981 tgggctggcc aaactgctgg gtgcggaaga gaaagaatac catgcagaag gaggcaaagt
     2041 gcctatcaag tggatggcat tggaatcaat tttacacaga atctataccc accagagtga
     2101 tgtctggagc tacggggtga ctgtttggga gttgatgacc tttggatcca agccatatga
     2161 cggaatccct gccagcgaga tctcctccat cctggagaaa ggagaacgcc tccctcagcc
     2221 acccatatgt accatcgatg tctacatgat catggtcaag tgctggatga tagacgcaga
     2281 tagtcgccca aagttccgtg agttgatcat cgaattctcc aaaatggccc gagaccccca
     2341 gcgctacctt tagctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     2401 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     2461 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     2521 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     2581 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     2641 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     2701 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2761 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2821 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2881 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2941 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3001 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3061 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     3121 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3181 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3241 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3301 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3361 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3421 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     3481 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     3541 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     3601 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     3661 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     3721 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     3781 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     3841 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     3901 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     3961 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4021 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4081 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4141 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4201 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4261 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     4321 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     4381 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     4441 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     4501 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4561 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     4621 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4681 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     4741 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     4801 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4861 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4921 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4981 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5041 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5101 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5161 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5221 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5281 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5341 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5401 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5461 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5521 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5581 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5641 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5701 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5761 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5821 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5881 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5941 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6001 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6061 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6121 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6181 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6241 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6301 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6361 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6421 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6481 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6541 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6601 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6661 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6721 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     6781 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
//
