LOCUS       V042133                10815 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042133
VERSION     V042133
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..5495
                     /label="GLI3(NM_000168)"
                     /note="GLI3(NM_000168)"
                     /gene="GLI3"
     CDS             5502..5567
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    5591..6179
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    6245..6293
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6495..6923
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6937..7266
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7333..8127
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8303..8424
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8473..8489)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8497..8513
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8521..8551)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8566..8587
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8875..9463)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9634..10494)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10495..10599)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgga ggcccagtcc cacagctcca
      781 cgaccactga aaagaaaaaa gttgagaatt ccatagtgaa gtgctccact cgaacagatg
      841 tgagcgagaa agccgttgcc tccagcacca cttctaatga ggatgaaagt cctggacaga
      901 cttatcacag agagagaaga aacgcaatca ctatgcagcc acagaatgtc caggggctca
      961 gcaaagtcag tgaggaacct tcaacatcga gtgacgagag ggcctcattg atcaagaaag
     1021 agatccatgg gtccctgcca cacgtggcgg agccctctgt gccgtaccgc gggacggtgt
     1081 ttgccatgga ccccaggaat ggttacatgg agccccacta ccaccctcct catcttttcc
     1141 ctgccttcca tcctcctgta ccaattgatg ccagacatca tgagggccgt taccattacg
     1201 atccatctcc gattcctcca ttgcatatga cttccgcctt atctagtagc cctacgtatc
     1261 cggacctgcc cttcattagg atctccccac accggaaccc cactgctgct tccgagtctc
     1321 ccttcagccc tccacatccc tacattaatc cctacatgga ctatatccgc tccttgcaca
     1381 gcagcccatc gctctccatg atctcagcaa cccgtgggct gagccctaca gatgcgcccc
     1441 atgcaggagt cagcccagca gaatactatc atcagatggc cctgctaact ggccagcgca
     1501 gcccctatgc agacattatt ccctcagctg ccaccgccgg cacgggggcc atccacatgg
     1561 aatatcttca tgctatggat agcaccagat tctccagccc caggctgtca gccaggccga
     1621 gccgaaaacg tacactgtcc atatcaccac tctccgatca tagctttgac cttcagacca
     1681 tgataaggac gtctcccaac tccttggtca cgattctcaa taattcccgt agcagctctt
     1741 cagcaagtgg ctcctatggt cacttatctg caagtgcaat cagccctgcc ttgagcttca
     1801 cctactcttc cgcgcccgtc tctctccaca tgcatcagca gatcctaagc cgacaacaga
     1861 gcttaggttc agcctttgga cacagccctc cactcatcca ccctgcccca acttttccaa
     1921 cacagaggcc tattccaggg atccctacgg ttctgaaccc cgtccaggtc agctccggcc
     1981 cttctgagtc ctcacagaac aagcccacga gtgagtctgc agtgagcagc actggtgacc
     2041 cgatgcacaa caagaggtcc aagatcaaac ccgatgaaga cctccccagc ccaggggctc
     2101 gggggcagca ggaacagccc gaaggaacaa cccttgtcaa ggaggaaggg gacaaagatg
     2161 aaagcaaaca ggagcctgaa gtcatctatg agacaaactg ccactgggaa ggctgcgcga
     2221 gggagttcga cacccaagag cagcttgtgc accatataaa taacgaccat attcatggag
     2281 agaagaagga gttcgtgtgc aggtggctgg actgctcaag agagcagaaa cccttcaaag
     2341 cccagtatat gttggtagtg catatgagaa gacacacggg cgagaagcct cacaaatgca
     2401 cttttgaagg ttgcacaaag gcctactcga gactagaaaa cttgaaaaca cacttgagat
     2461 ctcacactgg agagaaacca tacgtctgtg agcacgaagg ttgcaacaag gctttctcaa
     2521 atgcctctga tcgcgccaaa caccaaaaca gaacgcattc caatgagaaa ccatatgtgt
     2581 gcaaaatccc aggctgcact aagcgttaca cagacccaag ctccctccgg aaacatgtga
     2641 agacagtgca tggcccagag gctcatgtca ccaagaagca gcgaggggac atccatcctc
     2701 ggccgccacc cccgagagat tccggcagcc attcacagtc caggtcgcct ggccgaccga
     2761 ctcagggagc ccttggtgag cagcaggacc tcagcaacac tacctcaaag cgggaagaat
     2821 gcctccaggt gaaaaccgtc aaggcagaga agccaatgac atctcagcca agccctggtg
     2881 gtcagtcttc atgcagcagc caacagtccc ccatcagcaa ctattccaac agtgggctcg
     2941 agcttcctct gaccgatgga ggtagtatag gagacctcag tgccatcgat gaaaccccaa
     3001 tcatggactc aaccatttcc actgcaacca cagcccttgc tttgcaagcc aggagaaacc
     3061 cggcagggac caaatggatg gagcacgtaa aactagaaag gctaaaacaa gtgaatggaa
     3121 tgtttccgcg actgaacccc attctacccc ctaaagcccc tgcggtctct cctctcatag
     3181 gaaatggcac acagtccaac aacacctgca gcttgggtgg gcccatgacg cttctcccgg
     3241 gcagaagcga cctctctggg gtggacgtca ctatgctgaa catgctcaac agaagggaca
     3301 gcagcgccag caccatcagc tcggcctacc tgagcagccg ccgctcctca gggatctcgc
     3361 cctgcttctc cagccgccgc tccagcgagg cgtcacaggc cgagggccgg ccgcagaacg
     3421 tgagcgtggc cgactcctac gaccccatct ccaccgacgc ctcgcgccgc tccagcgaag
     3481 ccagccagag cgacggcctg cccagcctgc tcagcctcac gcccgcccag cagtaccgcc
     3541 tcaaggccaa gtacgcggct gccacaggag ggccgccgcc gacgcccctg cccaacatgg
     3601 agaggatgag cctgaagacg cgcctggcgc tgctcgggga tgccctcgag cctggcgtgg
     3661 ccctgcctcc agttcatgcc ccgaggaggt gcagcgacgg gggagcccac ggctacgggc
     3721 ggcgccacct gcagccgcac gatgcgccgg gccacggcgt gaggagggcc agcgacccgg
     3781 tgcggacagg ctccgagggc ctggccctgc ctcgtgtgcc gcgcttcagc agcctcagca
     3841 gctgcaaccc cccggcgatg gccacgtccg cggagaagcg cagtctcgtg cttcagaatt
     3901 acacgcggcc cgagggcggc cagtcccgaa acttccactc gtccccctgt cctcccagca
     3961 tcaccgagaa cgtcaccctg gagtccctga ccatggacgc tgatgccaac ctgaacgatg
     4021 aggatttcct gccggacgac gtggtgcagt atttaaattc ccagaaccaa gcagggtacg
     4081 agcagcactt ccccagcgcc ctcccggacg acagcaaagt gccccacggg cccggtgact
     4141 ttgacgcgcc cgggctgcca gacagccacg ctggccagca gttccatgcc ctcgagcagc
     4201 cctgccccga gggcagcaaa accgacctgc ccattcagtg gaacgaagtc agctccggaa
     4261 gcgccgacct gtcctcctcc aagctcaagt gtgggccgcg gcccgctgtg ccgcagactc
     4321 gcgcctttgg gttctgcaac ggcatggtcg tccacccgca gaaccccttg aggagcgggc
     4381 ctgctggggg ctatcagacc ctcggggaga acagcaaccc ctacggtggc ccagagcact
     4441 tgatgctcca caacagcccc ggaagtggca ccagtggaaa cgccttccat gaacagccct
     4501 gtaaggcccc gcagtatggg aactgtctca acaggcagcc agtggcccct ggtgcactcg
     4561 acggtgcctg tggtgccggg attcaagcct caaagctgaa gagcaccccc atgcaaggga
     4621 gcgggggcca gctgaatttc ggcctgccgg tagcgccaaa tgagtcagct ggcagcatgg
     4681 tgaatggcat gcagaaccag gacccagtgg gacaggggta cctggctcac cagctcctcg
     4741 gcgacagcat gcagcacccg ggggcaggcc gccccggtca gcagatgctt gggcagatta
     4801 gtgctacctc acacatcaac atctaccaag ggccagagag ctgcctgcca ggggctcacg
     4861 gcatgggcag ccagccgtca agcttggcag ttgtcagggg ctaccagcca tgtgccagct
     4921 ttgggggcag caggcgccag gctatgccga gggacagcct tgctctgcag tcaggacagc
     4981 tcagtgacac aagtcagacc tgcagggtga atggtatcaa gatggagatg aaagggcagc
     5041 cccatccgct gtgctctaat ctgcagaatt actctggtca gttctatgac caaaccgtgg
     5101 gcttcagtca gcaagacacg aaagctggtt cattctctat ttcagacgcc agctgcctgc
     5161 tacaggggac cagcgccaaa aactctgagt tactttcccc aggtgctaat caggtgacaa
     5221 gcacagtgga cagcctcgac agccatgacc tggaaggggt acagattgac ttcgatgcca
     5281 tcatagacga tggggaccac tccagcctga tgtcgggggc cctgagccca agtatcattc
     5341 agaacctttc ccatagctcc tcccgcctca ccacgcctcg ggcgtccctc ccattcccag
     5401 cgctgtccat gagcaccacc aacatggcta tcggggacat gagttctttg ctgacctccc
     5461 tagcggaaga aagcaaattc cttgcagtta tgcaaggatc cgactacaaa gaccatgacg
     5521 gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag tgagggcccg
     5581 atatctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5641 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5701 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5761 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5821 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5881 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5941 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     6001 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6061 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6121 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     6181 tggaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     6241 atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg
     6301 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     6361 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     6421 gctcgcagcc aacgtcgggg cggcaggccc tgccatagca gatctgcgca gctggggctc
     6481 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6541 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6601 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt
     6661 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     6721 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     6781 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     6841 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat
     6901 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     6961 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7021 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7081 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7141 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7201 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7261 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     7321 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     7381 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     7441 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     7501 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     7561 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     7621 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     7681 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     7741 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     7801 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     7861 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     7921 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     7981 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     8041 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     8101 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca
     8161 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     8221 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     8281 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     8341 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8401 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     8461 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8521 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8581 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8641 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8701 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8761 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8821 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8881 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8941 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9001 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9061 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9121 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9181 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9241 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9301 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9361 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9421 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9481 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9541 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9601 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9661 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9721 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9781 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9841 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9901 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9961 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10021 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10081 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10141 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10201 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10261 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10321 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10381 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10441 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10501 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10561 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10621 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc
    10681 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
    10741 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
    10801 aagaatctgc ttagg
//
