LOCUS V019789 7607 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019789 VERSION V019789 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 683..2287 /label="Fmo2(NM_018881)" /note="Fmo2(NM_018881)" /gene="Fmo2" CDS 2294..2359 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 2383..2971 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3037..3085 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3287..3715 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3729..4058 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4125..4919 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5095..5216 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5265..5281) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5289..5305 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5313..5343) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5358..5379 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5667..6255) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6426..7286) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7287..7391) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccgcca ccatggcaaa gaaggttgtg gtgattggag caggggtcag 721 tggcctaatc tctctgaagt gctgcgtgga cgagggtctg gagcccacct gcttcgaaag 781 gactgaagat atagggggac tgtggagatt caaagaaaac gtggaagatg gccgcgctag 841 catctaccgc tctgtcatta ccaacactag caaagaaatg tcctgtttca gtgacttccc 901 gatgccggag gacttcccca acttcctgca caactctaaa ctcctggaat attttaggat 961 ctttgccaag aaatttgatc tcctaaaata tattcagttt cagaccactg tcattagtgt 1021 gaaaaaacgc ccggacttcg catcttctgg gcaatgggaa gtttatactc agagcaacgg 1081 aaaggagcaa cgcactgtct ttgacgctgt tatggtttgc agcggccatc acatccagcc 1141 tcacctgcca ctcaagtcat tcccaggtat tgagaggttc cgaggccagt atttccatag 1201 ccgcgaatac aagcacccag tggggtttga ggggaaacgc atcctggtgg ttggaatagg 1261 aaactcagct gcagatattg cctctgaact gagtaagacg gccgcgcagg tgtttgtcag 1321 caccagacat ggatcctggg tcatgagtcg aatctctgaa gatggctacc cttgggacat 1381 ggtgttccac actagattta gctccatgct ccgaaatgtc ctgccccgca cggtcgtcaa 1441 gtggatgatg gaacaacaga tgaatcgatg gttcaaccat gaaaattatg gtctggtgcc 1501 tcagaacaaa taccttatga aagaacctgt gctaaacgac gacctcccca gccgcctgct 1561 gtacggagcc atcaaagtaa aaacacgggt gaaggagctc acggagacag ctgtggtctt 1621 cgaggatggc acggtggagg aagacgtgga tatcattgtc tttgccacgg ggtatacgtt 1681 ctctttttct ttcctggaag actcacttgt taaagtggag gataataggg tttccctgta 1741 caaagccatg tttccccctc acctggagaa gccaaccctt gcgtgcatag gtctcatcca 1801 gcctctaggc tccatcttcc caaccgtaga gcttcaggcg cgctgggcga caagagtttt 1861 caaaggcttg tgtagcttgc cttcggagac gactatgatg gcagacattg ttgaaagaaa 1921 tgaaaaaaga gttaacctgt ttgggaaaag ccagagtcag atactgcaga ccaactacgt 1981 tgactacctg gatgaactcg ccttggagat aggtgcaaag ccagatttcg tctctctctt 2041 tttcaaagac cctaaactgg ctgtgaaact ctacttcgga ccctgtaatt cctaccagta 2101 ccgcctggtt ggacctgggc agtgggaagg agccaggaac gccatcctca cccagaagca 2161 gaggatcctg aaacccttaa agacccggac tctccagtcc tctgatagtg ccccagtgtc 2221 tttcctgctc aagatcctgg ggctgctcgc tgttgttctg gccttctttt tccaacttca 2281 ggggtttctc gaggactaca aagaccatga cggtgattat aaagatcatg acatcgacta 2341 caaggatgac gatgacaagt agtgagggcc cgatatctcg acaatcaacc tctggattac 2401 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 2461 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 2521 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 2581 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 2641 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 2701 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 2761 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg 2821 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 2881 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg 2941 agtcggatct ccctttgggc cgcctccccg cctggaaacg ggggaggcta actgaaacac 3001 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa 3061 cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct 3121 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca 3181 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc 3241 cctgccatag cagatctgcg cagctggggc tctagggggt atccccacgc gccctgtagc 3301 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 3361 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 3421 ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac 3481 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 3541 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 3601 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg 3661 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc 3721 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 3781 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 3841 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 3901 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 3961 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 4021 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 4081 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 4141 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 4201 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 4261 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 4321 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 4381 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 4441 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 4501 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 4561 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 4621 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg 4681 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 4741 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 4801 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 4861 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 4921 cgggactctg gggttcgcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 4981 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 5041 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg 5101 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 5161 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 5221 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 5281 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 5341 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 5401 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 5461 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 5521 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 5581 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 5641 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 5701 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 5761 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 5821 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat 5881 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 5941 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 6001 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 6061 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 6121 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 6181 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 6241 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 6301 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 6361 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 6421 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 6481 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 6541 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 6601 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 6661 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 6721 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 6781 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6841 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6901 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 6961 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 7021 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 7081 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 7141 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 7201 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 7261 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 7321 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 7381 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc 7441 ccgatcccct atggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt 7501 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta 7561 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagg //