LOCUS V030244 5667 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030244 VERSION V030244 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..1814 /label="Fitm2(NM_173397)" /note="Fitm2(NM_173397)" /gene="Fitm2" CDS 1833..1862 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 1881..1904 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(1924..1940) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 1951..2573 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2722..3310) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3639..3686 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(3918..4712) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4747..5104) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5106..5210) /gene="bla" /label="AmpR promoter" rep_origin 5237..5665 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat ggagcacctg gagcgctgcg cgtggtttct ccgcgggacg ctggtgaggg 1081 cgacggtgcg gcgccacctg ccctgggcgc tggtggccgc catgctggcg ggctcggtcg 1141 tcaaggagct gtccccgctg cccgagagct acctcagcaa caagcgcaac gtcctcaacg 1201 tgtattttgt taaattggcc tgggcctgga cagtctgtct cctcctgcct ttcattgccc 1261 ttaccaacta ccacctgacg ggcaagacca gcctggtcct gcggcggttg agcaccctcc 1321 tcgtgggcac agccatctgg tatatctgca cagccctctt ctccaacatt gagcactata 1381 caggcagctg ctaccagtcc ccggccctgg agggcatcag acaggagcat cgcagcaagc 1441 agcagtgcca ccgggaaggg ggcttctggc atggctttga catctcgggc cactccttcc 1501 tcttgacctt ctgtgcgctc atgattgtgg aagagatggc cgtgctgcat gaggtgaaga 1561 cggaccgcgg ccaccacctc catgccgcca tcaccaccct ggttgtcgcc ctgggtttcc 1621 tgaccttcat ctgggtgtgg atgtttctgt gcacagccgt ttatttccac gacttgaccc 1681 agaaagtgtt cggcaccatg tttggtttgt tgggctggta cggaacatat gggtattggt 1741 atctgaagtc tttctctcca ggacttcctc cccagagttg tagtttgact ttgaagcgag 1801 atacttacaa gaaaacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc 1861 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc 1921 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg 1981 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 2041 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 2101 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 2161 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 2221 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 2281 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 2341 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 2401 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac 2461 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg 2521 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg 2581 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 2641 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 2701 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 2761 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 2821 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 2881 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 2941 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 3001 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 3061 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 3121 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 3181 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 3241 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 3301 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 3361 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 3421 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc 3481 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg 3541 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc 3601 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg 3661 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt 3721 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg 3781 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa 3841 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc 3901 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 3961 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 4021 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc 4081 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 4141 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg 4201 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 4261 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 4321 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 4381 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 4441 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 4501 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg 4561 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 4621 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga 4681 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga 4741 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc 4801 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg 4861 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat 4921 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga 4981 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg 5041 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc 5101 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 5161 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 5221 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 5281 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 5341 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 5401 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 5461 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 5521 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 5581 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 5641 atttaacaaa aatttaacgc gaatttt //