LOCUS       V042541                13506 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042541
VERSION     V042541
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..8186
                     /label="FN1(NM_212482)"
                     /note="FN1(NM_212482)"
                     /gene="FN1"
     CDS             8193..8258
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    8282..8870
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    8936..8984
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      9186..9614
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9628..9957
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             10024..10818
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    10994..11115
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(11164..11180)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11188..11204
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11212..11242)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11257..11278
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11566..12154)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12325..13185)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13186..13290)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgct taggggtccg gggcccgggc
      781 tgctgctgct ggccgtccag tgcctgggga cagcggtgcc ctccacggga gcctcgaaga
      841 gcaagaggca ggctcagcaa atggttcagc cccagtcccc ggtggctgtc agtcaaagca
      901 agcccggttg ttatgacaat ggaaaacact atcagataaa tcaacagtgg gagcggacct
      961 acctaggcaa tgcgttggtt tgtacttgtt atggaggaag ccgaggtttt aactgcgaga
     1021 gtaaacctga agctgaagag acttgctttg acaagtacac tgggaacact taccgagtgg
     1081 gtgacactta tgagcgtcct aaagactcca tgatctggga ctgtacctgc atcggggctg
     1141 ggcgagggag aataagctgt accatcgcaa accgctgcca tgaagggggt cagtcctaca
     1201 agattggtga cacctggagg agaccacatg agactggtgg ttacatgtta gagtgtgtgt
     1261 gtcttggtaa tggaaaagga gaatggacct gcaagcccat agctgagaag tgttttgatc
     1321 atgctgctgg gacttcctat gtggtcggag aaacgtggga gaagccctac caaggctgga
     1381 tgatggtaga ttgtacttgc ctgggagaag gcagcggacg catcacttgc acttctagaa
     1441 atagatgcaa cgatcaggac acaaggacat cctatagaat tggagacacc tggagcaaga
     1501 aggataatcg aggaaacctg ctccagtgca tctgcacagg caacggccga ggagagtgga
     1561 agtgtgagag gcacacctct gtgcagacca catcgagcgg atctggcccc ttcaccgatg
     1621 ttcgtgcagc tgtttaccaa ccgcagcctc acccccagcc tcctccctat ggccactgtg
     1681 tcacagacag tggtgtggtc tactctgtgg ggatgcagtg gctgaagaca caaggaaata
     1741 agcaaatgct ttgcacgtgc ctgggcaacg gagtcagctg ccaagagaca gctgtaaccc
     1801 agacttacgg tggcaactca aatggagagc catgtgtctt accattcacc tacaatggca
     1861 ggacgttcta ctcctgcacc acagaagggc gacaggacgg acatctttgg tgcagcacaa
     1921 cttcgaatta tgagcaggac cagaaatact ctttctgcac agaccacact gttttggttc
     1981 agactcgagg aggaaattcc aatggtgcct tgtgccactt ccccttccta tacaacaacc
     2041 acaattacac tgattgcact tctgagggca gaagagacaa catgaagtgg tgtgggacca
     2101 cacagaacta tgatgccgac cagaagtttg ggttctgccc catggctgcc cacgaggaaa
     2161 tctgcacaac caatgaaggg gtcatgtacc gcattggaga tcagtgggat aagcagcatg
     2221 acatgggtca catgatgagg tgcacgtgtg ttgggaatgg tcgtggggaa tggacatgca
     2281 ttgcctactc gcagcttcga gatcagtgca ttgttgatga catcacttac aatgtgaacg
     2341 acacattcca caagcgtcat gaagaggggc acatgctgaa ctgtacatgc ttcggtcagg
     2401 gtcggggcag gtggaagtgt gatcccgtcg accaatgcca ggattcagag actgggacgt
     2461 tttatcaaat tggagattca tgggagaagt atgtgcatgg tgtcagatac cagtgctact
     2521 gctatggccg tggcattggg gagtggcatt gccaaccttt acagacctat ccaagctcaa
     2581 gtggtcctgt cgaagtattt atcactgaga ctccgagtca gcccaactcc caccccatcc
     2641 agtggaatgc accacagcca tctcacattt ccaagtacat tctcaggtgg agacctaaaa
     2701 attctgtagg ccgttggaag gaagctacca taccaggcca cttaaactcc tacaccatca
     2761 aaggcctgaa gcctggtgtg gtatacgagg gccagctcat cagcatccag cagtacggcc
     2821 accaagaagt gactcgcttt gacttcacca ccaccagcac cagcacacct gtgaccagca
     2881 acaccgtgac aggagagacg actccctttt ctcctcttgt ggccacttct gaatctgtga
     2941 ccgaaatcac agccagtagc tttgtggtct cctgggtctc agcttccgac accgtgtcgg
     3001 gattccgggt ggaatatgag ctgagtgagg agggagatga gccacagtac ctggatcttc
     3061 caagcacagc cacttctgtg aacatccctg acctgcttcc tggccgaaaa tacattgtaa
     3121 atgtctatca gatatctgag gatggggagc agagtttgat cctgtctact tcacaaacaa
     3181 cagcgcctga tgcccctcct gacacgactg tggaccaagt tgatgacacc tcaattgttg
     3241 ttcgctggag cagaccccag gctcccatca cagggtacag aatagtctat tcgccatcag
     3301 tagaaggtag cagcacagaa ctcaaccttc ctgaaactgc aaactccgtc accctcagtg
     3361 acttgcaacc tggtgttcag tataacatca ctatctatgc tgtggaagaa aatcaagaaa
     3421 gtacacctgt tgtcattcaa caagaaacca ctggcacccc acgctcagat acagtgccct
     3481 ctcccaggga cctgcagttt gtggaagtga cagacgtgaa ggtcaccatc atgtggacac
     3541 cgcctgagag tgcagtgacc ggctaccgtg tggatgtgat ccccgtcaac ctgcctggcg
     3601 agcacgggca gaggctgccc atcagcagga acacctttgc agaagtcacc gggctgtccc
     3661 ctggggtcac ctattacttc aaagtctttg cagtgagcca tgggagggag agcaagcctc
     3721 tgactgctca acagacaacc aaactggatg ctcccactaa cctccagttt gtcaatgaaa
     3781 ctgattctac tgtcctggtg agatggactc cacctcgggc ccagataaca ggataccgac
     3841 tgaccgtggg ccttacccga agaggacagc ccaggcagta caatgtgggt ccctctgtct
     3901 ccaagtaccc actgaggaat ctgcagcctg catctgagta caccgtatcc ctcgtggcca
     3961 taaagggcaa ccaagagagc cccaaagcca ctggagtctt taccacactg cagcctggga
     4021 gctctattcc accttacaac accgaggtga ctgagaccac cattgtgatc acatggacgc
     4081 ctgctccaag aattggtttt aagctgggtg tacgaccaag ccagggagga gaggcaccac
     4141 gagaagtgac ttcagactca ggaagcatcg ttgtgtccgg cttgactcca ggagtagaat
     4201 acgtctacac catccaagtc ctgagagatg gacaggaaag agatgcgcca attgtaaaca
     4261 aagtggtgac accattgtct ccaccaacaa acttgcatct ggaggcaaac cctgacactg
     4321 gagtgctcac agtctcctgg gagaggagca ccaccccaga cattactggt tatagaatta
     4381 ccacaacccc tacaaacggc cagcagggaa attctttgga agaagtggtc catgctgatc
     4441 agagctcctg cacttttgat aacctgagtc ccggcctgga gtacaatgtc agtgtttaca
     4501 ctgtcaagga tgacaaggaa agtgtcccta tctctgatac catcatccca gaggtgcccc
     4561 aactcactga cctaagcttt gttgatataa ccgattcaag catcggcctg aggtggaccc
     4621 cgctaaactc ttccaccatt attgggtacc gcatcacagt agttgcggca ggagaaggta
     4681 tccctatttt tgaagatttt gtggactcct cagtaggata ctacacagtc acagggctgg
     4741 agccgggcat tgactatgat atcagcgtta tcactctcat taatggcggc gagagtgccc
     4801 ctactacact gacacaacaa acggctgttc ctcctcccac tgacctgcga ttcaccaaca
     4861 ttggtccaga caccatgcgt gtcacctggg ctccaccccc atccattgat ttaaccaact
     4921 tcctggtgcg ttactcacct gtgaaaaatg aggaagatgt tgcagagttg tcaatttctc
     4981 cttcagacaa tgcagtggtc ttaacaaatc tcctgcctgg tacagaatat gtagtgagtg
     5041 tctccagtgt ctacgaacaa catgagagca cacctcttag aggaagacag aaaacaggtc
     5101 ttgattcccc aactggcatt gacttttctg atattactgc caactctttt actgtgcact
     5161 ggattgctcc tcgagccacc atcactggct acaggatccg ccatcatccc gagcacttca
     5221 gtgggagacc tcgagaagat cgggtgcccc actctcggaa ttccatcacc ctcaccaacc
     5281 tcactccagg cacagagtat gtggtcagca tcgttgctct taatggcaga gaggaaagtc
     5341 ccttattgat tggccaacaa tcaacagttt ctgatgttcc gagggacctg gaagttgttg
     5401 ctgcgacccc caccagccta ctgatcagct gggatgctcc tgctgtcaca gtgagatatt
     5461 acaggatcac ttacggagag acaggaggaa atagccctgt ccaggagttc actgtgcctg
     5521 ggagcaagtc tacagctacc atcagcggcc ttaaacctgg agttgattat accatcactg
     5581 tgtatgctgt cactggccgt ggagacagcc ccgcaagcag caagccaatt tccattaatt
     5641 accgaacaga aattgacaaa ccatcccaga tgcaagtgac cgatgttcag gacaacagca
     5701 ttagtgtcaa gtggctgcct tcaagttccc ctgttactgg ttacagagta accaccactc
     5761 ccaaaaatgg accaggacca acaaaaacta aaactgcagg tccagatcaa acagaaatga
     5821 ctattgaagg cttgcagccc acagtggagt atgtggttag tgtctatgct cagaatccaa
     5881 gcggagagag tcagcctctg gttcagactg cagtaaccaa cattgatcgc cctaaaggac
     5941 tggcattcac tgatgtggat gtcgattcca tcaaaattgc ttgggaaagc ccacaggggc
     6001 aagtttccag gtacagggtg acctactcga gccctgagga tggaatccat gagctattcc
     6061 ctgcacctga tggtgaagaa gacactgcag agctgcaagg cctcagaccg ggttctgagt
     6121 acacagtcag tgtggttgcc ttgcacgatg atatggagag ccagcccctg attggaaccc
     6181 agtccacagc tattcctgca ccaactgacc tgaagttcac tcaggtcaca cccacaagcc
     6241 tgagcgccca gtggacacca cccaatgttc agctcactgg atatcgagtg cgggtgaccc
     6301 ccaaggagaa gaccggacca atgaaagaaa tcaaccttgc tcctgacagc tcatccgtgg
     6361 ttgtatcagg acttatggtg gccaccaaat atgaagtgag tgtctatgct cttaaggaca
     6421 ctttgacaag cagaccagct cagggagttg tcaccactct ggagaatgtc agcccaccaa
     6481 gaagggctcg tgtgacagat gctactgaga ccaccatcac cattagctgg agaaccaaga
     6541 ctgagacgat cactggcttc caagttgatg ccgttccagc caatggccag actccaatcc
     6601 agagaaccat caagccagat gtcagaagct acaccatcac aggtttacaa ccaggcactg
     6661 actacaagat ctacctgtac accttgaatg acaatgctcg gagctcccct gtggtcatcg
     6721 acgcctccac tgccattgat gcaccatcca acctgcgttt cctggccacc acacccaatt
     6781 ccttgctggt atcatggcag ccgccacgtg ccaggattac cggctacatc atcaagtatg
     6841 agaagcctgg gtctcctccc agagaagtgg tccctcggcc ccgccctggt gtcacagagg
     6901 ctactattac tggcctggaa ccgggaaccg aatatacaat ttatgtcatt gccctgaaga
     6961 ataatcagaa gagcgagccc ctgattggaa ggaaaaagac agacgagctt ccccaactgg
     7021 taacccttcc acaccccaat cttcatggac cagagatctt ggatgttcct tccacagttc
     7081 aaaagacccc tttcgtcacc caccctgggt atgacactgg aaatggtatt cagcttcctg
     7141 gcacttctgg tcagcaaccc agtgttgggc aacaaatgat ctttgaggaa catggtttta
     7201 ggcggaccac accgcccaca acggccaccc ccataaggca taggccaaga ccatacccgc
     7261 cgaatgtagg tgaggaaatc caaattggtc acatccccag ggaagatgta gactatcacc
     7321 tgtacccaca cggtccggga ctcaatccaa atgcctctac aggacaagaa gctctctctc
     7381 agacaaccat ctcatgggcc ccattccagg acacttctga gtacatcatt tcatgtcatc
     7441 ctgttggcac tgatgaagaa cccttacagt tcagggttcc tggaacttct accagtgcca
     7501 ctctgacagg cctcaccaga ggtgccacct acaacgtcat agtggaggca ctgaaagacc
     7561 agcagaggca taaggttcgg gaagaggttg ttaccgtggg caactctgtc aacgaaggct
     7621 tgaaccaacc tacggatgac tcgtgctttg acccctacac agtttcccat tatgccgttg
     7681 gagatgagtg ggaacgaatg tctgaatcag gctttaaact gttgtgccag tgcttaggct
     7741 ttggaagtgg tcatttcaga tgtgattcat ctagatggtg ccatgacaat ggtgtgaact
     7801 acaagattgg agagaagtgg gaccgtcagg gagaaaatgg ccagatgatg agctgcacat
     7861 gtcttgggaa cggaaaagga gaattcaagt gtgaccctca tgaggcaacg tgttatgatg
     7921 atgggaagac ataccacgta ggagaacagt ggcagaagga atatctcggt gccatttgct
     7981 cctgcacatg ctttggaggc cagcggggct ggcgctgtga caactgccgc agacctgggg
     8041 gtgaacccag tcccgaaggc actactggcc agtcctacaa ccagtattct cagagatacc
     8101 atcagagaac aaacactaat gttaattgcc caattgagtg cttcatgcct ttagatgtac
     8161 aggctgacag agaagattcc cgagagggat ccgactacaa agaccatgac ggtgattata
     8221 aagatcatga catcgactac aaggatgacg atgacaagta gtgagggccc gatatctcga
     8281 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     8341 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     8401 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     8461 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     8521 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     8581 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     8641 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     8701 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     8761 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     8821 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaaacgg
     8881 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     8941 ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg
     9001 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     9061 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     9121 caacgtcggg gcggcaggcc ctgccatagc agatctgcgc agctggggct ctagggggta
     9181 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     9241 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     9301 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg
     9361 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     9421 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     9481 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     9541 tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     9601 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     9661 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     9721 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9781 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9841 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     9901 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9961 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
    10021 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
    10081 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
    10141 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
    10201 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
    10261 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
    10321 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
    10381 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
    10441 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
    10501 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
    10561 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
    10621 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
    10681 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
    10741 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
    10801 cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc
    10861 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    10921 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    10981 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    11041 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    11101 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
    11161 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    11221 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    11281 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    11341 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    11401 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    11461 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    11521 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    11581 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    11641 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    11701 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    11761 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    11821 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    11881 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    11941 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    12001 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    12061 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    12121 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    12181 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    12241 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    12301 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    12361 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    12421 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    12481 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    12541 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    12601 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    12661 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    12721 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    12781 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    12841 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    12901 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    12961 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    13021 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    13081 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    13141 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    13201 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    13261 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    13321 cgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
    13381 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
    13441 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
    13501 cttagg
//
