LOCUS       V020891                14449 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020891
VERSION     V020891
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             649..1365
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1387..9192
                     /label="FLNB(NM_001457)"
                     /note="FLNB(NM_001457)"
                     /gene="FLNB"
     misc_feature    9225..9813
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    9879..9927
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      10129..10557
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        10571..10900
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             10967..11761
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    11937..12058
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(12107..12123)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12131..12147
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12155..12185)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12200..12221
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12509..13097)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13268..14128)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14129..14233)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgccaccat ggtgagcaag
      661 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      721 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      781 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      841 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      901 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      961 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1021 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1081 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1141 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1201 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1261 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1321 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggagg agggggaagc
     1381 ggtaccatgc cggtaaccga gaaggatcta gctgaggacg cgccttggaa gaagatccag
     1441 cagaacacgt tcacacgctg gtgcaacgag cacctcaagt gcgtgaacaa acgcatcggc
     1501 aacctgcaga ccgacctgag cgacgggctg cggctcatcg cgctgctcga ggtgctcagc
     1561 cagaagcgca tgtaccgcaa gtaccatcag cggcccacct ttcgccagat gcagctcgag
     1621 aatgtgtccg tggcgctcga gttcctggac cgtgagagca tcaagctcgt gtccatcgat
     1681 agcaaagcca ttgtggatgg gaacctgaag ctcatcttgg gtctggtgtg gacgctgatc
     1741 ctccactact ccatctccat gcccgtgtgg gaggatgaag gggatgatga tgccaagaag
     1801 cagacgccaa agcagaggct gctggggtgg attcagaaca agatccccta cttgcccatc
     1861 accaacttta accagaactg gcaagacggc aaagccctgg gagccctggt agacagctgt
     1921 gctccaggtc tgtgcccaga ctgggaatcc tgggacccgc agaagcctgt ggataatgca
     1981 cgagaagcca tgcagcaggc agatgactgg ctgggtgtcc cacaggtcat cactcctgaa
     2041 gaaatcattc acccggatgt ggacgagcac tcagttatga cttacctgtc ccagttcccc
     2101 aaagccaagc tcaagccggg ggctcctctc aaacccaaac tcaacccgaa gaaagccagg
     2161 gcctatggca gaggaatcga gcccactgga aacatggtga agcagccagc caagttcact
     2221 gtggacacca tcagcgccgg gcaaggagac gtgatggtgt ttgttgagga cccagaaggg
     2281 aacaaagagg aggcacaagt gacccctgac agtgacaaga acaagacata ctctgtggag
     2341 tatctgccca aggtcaccgg gctacacaaa gtcacagtcc tctttgcagg acagcacatc
     2401 tccaagagcc catttgaagt gagtgttgac aaggcccagg gagatgccag taaagtcact
     2461 gcaaaaggtc cagggttgga agctgtaggg aacatcgcca ataagcccac ctactttgac
     2521 atctatacgg caggagctgg tgtgggtgac attggtgtgg aggtggaaga tccccagggg
     2581 aagaacaccg tggagttgct cgtggaagac aaaggaaacc aggtgtatcg atgtgtgtac
     2641 aaacccatgc agcctggccc tcacgtggtc aagatcttct ttgctgggga cactattcct
     2701 aagagtccct tcgttgtgca ggttggggaa gcctgcaatc caaatgcctg ccgggccagt
     2761 ggccgaggcc tacaacccaa aggcgtccgt atccgggaga ccacagattt caaggttgac
     2821 accaaagctg caggaagtgg ggagctcggt gtaaccatga agggtcctaa gggtctggag
     2881 gagctggtga agcagaaaga ctttctggat ggggtctacg cattcgagta ttaccccagc
     2941 accccgggga gatacagcat tgccatcaca tgggggggac accacattcc aaagagcccc
     3001 tttgaagttc aagttggccc tgaagcgggt atgcagaaag tccgtgcttg gggccctggg
     3061 ctccatggtg ggattgtcgg gcggtcagcg gacttcgtgg tagaatccat tggctctgaa
     3121 gtggggtctc tggggtttgc cattgaaggc ccctctcagg caaagattga gtacaacgac
     3181 cagaatgatg gatcgtgtga tgtcaaatac tggcccaagg agcctggcga atatgctgtt
     3241 cacatcatgt gtgacgacga agacatcaag gacagcccgt acatggcctt catccaccca
     3301 gccacgggag gctacaaccc tgatctggtt cgagcatacg ggccaggttt ggagaaatct
     3361 ggatgcattg tcaacaacct ggccgagttc actgtggatc ctaaggatgc tggaaaagct
     3421 cccttaaaga tatttgctca ggatggggaa ggccaacgca ttgacatcca gatgaagaac
     3481 cggatggacg gcacatatgc atgctcatac accccggtga aggccatcaa gcacaccatt
     3541 gctgtggtct ggggaggcgt gaacatcccg cacagcccct acagggtcaa catcgggcaa
     3601 ggtagccatc ctcagaaggt caaagtgttt gggccaggtg tggagagaag tggtctgaag
     3661 gcaaatgaac ctacacactt cacggtggac tgtactgagg ctggggaagg tgatgtcagt
     3721 gttggcatta agtgtgatgc ccgggtgtta agtgaagatg aggaagacgt ggattttgac
     3781 attattcaca atgccaatga tacgttcaca gtcaaatatg tgcctcctgc tgctgggcga
     3841 tacactatca aagttctctt tgcatctcag gaaatccccg ccagcccttt cagagtcaaa
     3901 gttgaccctt cccacgatgc cagcaaagtg aaggcagaag gcccagggct cagcaaagca
     3961 ggtgtggaaa atgggaaacc gacccacttc actgtctaca ccaagggggc tgggaaagcc
     4021 ccgctcaacg tgcagttcaa cagccctctt cctggcgatg cagtgaagga tttggatatc
     4081 atcgataatt atgactactc tcacacggtt aaatatacac ccacccaaca gggcaacatg
     4141 caggttctgg tgacttacgg tggcgatccc atccctaaaa gccctttcac tgtgggtgtt
     4201 gctgcaccgc tggatctgag caagataaaa ctcaatgggc tggaaaacag ggtggaagtt
     4261 gggaaggatc aggagttcac cgttgatacc aggggggcag gaggccaggg gaagctggac
     4321 gtgacaatcc tcagcccctc tcggaaggtc gtgccatgcc tagtgacacc tgtgacaggc
     4381 cgggagaaca gcacggccaa gttcatccct cgggaggagg ggctgtatgc tgtagacgtg
     4441 acctacgatg gacaccctgt gcccgggagc ccctacacag tggaggcctc gctgccacca
     4501 gatcccagca aggtgaaggc ccacggtccc ggcctcgaag gtggtctcgt gggcaagcct
     4561 gccgagttca ccatcgatac caaaggagct ggtactggag gtctgggctt aacggtggaa
     4621 ggtccgtgcg aggccaaaat cgagtgctcc gacaatggtg atgggacctg ctccgtctct
     4681 taccttccca caaaacccgg ggagtacttc gtcaacatcc tctttgaaga agtccacata
     4741 cctgggtctc ccttcaaagc tgacattgaa atgccctttg acccctctaa agtcgtggca
     4801 tcggggccag gtctcgagca cgggaaggtg ggtgaagctg gcctccttag cgtcgactgc
     4861 tcggaagcgg gaccgggggc cctgggcctg gaagctgtct cggactcggg aacaaaagcc
     4921 gaagtcagta ttcagaacaa caaagatggc acctacgcgg tgacctacgt gcccctgacg
     4981 gccggcatgt acacgttgac catgaagtat ggtggcgaac tcgtgccaca cttccccgcc
     5041 cgggtcaagg tggagcccgc cgtggacacc agcaggatca aagtctttgg accaggaata
     5101 gaagggaaag atgtgttccg ggaagctacc accgacttta cagttgactc tcggccgctg
     5161 acccaggttg ggggtgacca catcaaggcc cacattgcca acccctcagg ggcctccacc
     5221 gagtgctttg tcacagacaa tgcggatggg acctaccagg tggaatacac accctttgag
     5281 aaaggtctcc atgtagtgga ggtgacatat gatgacgtgc ctatcccaaa cagtcccttc
     5341 aaggtggctg tcactgaagg ctgccagcca tctagggtgc aagcccaagg acctggattg
     5401 aaagaggcct ttaccaacaa gcccaatgtc ttcaccgtgg ttaccagagg cgcaggaatt
     5461 ggtgggcttg gcataactgt tgagggacca tcagagtcga agataaattg cagagacaac
     5521 aaggatggca gctgcagtgc tgagtacatt cctttcgcac cgggggatta cgatgttaat
     5581 atcacatatg gaggagccca catccccggc agccccttca gggttcctgt gaaggatgtt
     5641 gtggacccca gcaaggtcaa gattgccggc cccgggctgg gctcaggcgt ccgagcccgt
     5701 gtcctgcagt ccttcacggt ggacagcagc aaggctggcc tggctccgct ggaagtgagg
     5761 gttctgggcc cacgaggctt ggtggagcca gtgaacgtgg tggacaatgg agatggcaca
     5821 cacacagtaa cctacacccc atctcaggag ggaccttaca tggtctcagt taaatatgct
     5881 gatgaagaga ttcctcgcag tcccttcaag gtcaaggtcc ttcccacata tgatgccagc
     5941 aaagtgactg ccagtggccc cggccttagt tcctatggtg tgcctgccag tctacctgtg
     6001 gactttgcaa ttgatgcccg agatgccggg gaaggcctgc ttgctgttca aataacggac
     6061 caagaaggaa aacccaaaag agccattgtc catgacaata aagatggcac gtatgctgtc
     6121 acctacatcc ccgacaagac tgggcgctat atgattggag tcacctacgg gggtgacgac
     6181 atcccacttt ctccttatcg catccgagcc acacagacgg gtgatgccag caagtgcctg
     6241 gccacgggtc ctggaatcgc ctccactgtg aaaactggcg aagaagtagg ctttgtggtt
     6301 gatgccaaga ctgccgggaa gggtaaagtg acctgcacgg ttctgacccc agatggcact
     6361 gaggccgagg ccgatgtcat tgagaatgaa gatggaacct atgacatctt ctacacagct
     6421 gccaagccgg gcacatatgt gatctatgtg cgcttcggtg gtgttgatat tcctaacagc
     6481 cccttcactg tcatggccac agatggggaa gtcacagccg tggaggaggc accggtaaat
     6541 gcatgtcccc ctggattcag gccctgggtg accgaagagg cctatgtccc agtgagtgac
     6601 atgaacggcc tgggatttaa gccttttgac ctggtcattc cgtttgctgt caggaaagga
     6661 gaaatcactg gagaggtcca catgccttct gggaagacag ccacacctga gattgtggac
     6721 aacaaggacg gcacggtcac tgttagatat gcccccactg aggtcgggct ccatgagatg
     6781 cacatcaaat acatgggcag ccacatccct gagagcccac tccagttcta cgtgaactac
     6841 cccaacagtg gaagtgtttc tgcatacggt ccaggcctcg tgtatggagt ggccaacaaa
     6901 actgccacct tcaccatcgt cacagaggat gcaggagaag gtggtctgga cttggctatt
     6961 gagggcccct caaaagcaga aatcagctgc attgacaata aagatgggac atgcacagtg
     7021 acctacctgc cgactctgcc aggcgactac agcattctgg tcaagtacaa tgacaagcac
     7081 atccctggca gccccttcac agccaagatc acagatgaca gcaggcggtg ctcccaggtg
     7141 aagttgggct cagccgctga cttcctgctc gacatcagtg agactgacct cagcagcctg
     7201 acggccagca ttaaggcccc atctggccga gacgagccct gtctcctgaa gaggctgccc
     7261 aacaaccaca ttggcatctc cttcatcccc cgggaagtgg gcgaacatct ggtcagcatc
     7321 aagaaaaatg gcaaccatgt ggccaacagc cccgtgtcta tcatggtggt ccagtcggag
     7381 attggtgacg cccgccgagc caaagtctat ggccgcggcc tgtcagaagg ccggactttc
     7441 gagatgtctg acttcatcgt ggacacaagg gatgcaggtt atggtggcat atccttggcg
     7501 gtggaaggcc ccagcaaagt ggacatccag acggaggacc tggaagatgg cacctgcaaa
     7561 gtctcctact tccctaccgt gcctggggtt tatatcgtct ccaccaaatt cgctgacgag
     7621 cacgtgcctg ggagcccatt taccgtgaag atcagtgggg agggaagagt caaagagagc
     7681 atcacccgca ccagtcgggc cccgtccgtg gccactgtcg ggagcatttg tgacctgaac
     7741 ctgaaaatcc cagaaatcaa cagcagtgat atgtcggccc acgtcaccag cccctctggc
     7801 cgtgtgactg aggcagagat tgtgcccatg gggaagaact cacactgcgt ccggtttgtg
     7861 ccccaggaga tgggcgtgca cacggtcagc gtcaagtacc gtgggcagca cgtcaccggc
     7921 agccccttcc agttcaccgt ggggccactt ggtgaaggag gcgcccacaa ggtgcgggca
     7981 ggaggccctg gcctggagag aggagaagcg ggagtcccag ctgagttcag catttggacc
     8041 cgggaagcag gcgctggagg cctctccatc gctgttgagg gccccagtaa ggccgagatt
     8101 acattcgatg accataaaaa tgggtcgtgc ggtgtatctt atattgccca agagcctggt
     8161 aactacgagg tgtccatcaa gttcaatgat gagcacatcc cggaaagccc ctacctggtg
     8221 ccggtcatcg caccctccga cgacgcccgc cgcctcactg ttatgagcct tcaggaatcg
     8281 ggattaaaag ttaaccagcc agcatccttt gctataaggt tgaatggcgc aaaaggcaag
     8341 attgatgcaa aggtgcacag cccctctgga gccgtggagg agtgccacgt gtctgagctg
     8401 gagccagata agtatgctgt tcgcttcatc cctcatgaga atggtgtcca caccatcgat
     8461 gtcaagttca atgggagcca cgtggttgga agccccttca aagtgcgcgt tggggagcct
     8521 ggacaagcgg ggaaccctgc cctggtgtcc gcctatggca cgggactcga agggggcacc
     8581 acaggtatcc agtcggaatt ctttattaac accacccgag caggtccagg gacattatcc
     8641 gtcaccatcg aaggcccatc caaggttaaa atggattgcc aggaaacacc tgaagggtac
     8701 aaagtcatgt acacccccat ggctcctggt aactacctga tcagcgtcaa atacggtggg
     8761 cccaaccaca tcgtgggcag tcccttcaag gccaaggtga caggccagcg tctagttagc
     8821 cctggctcag ccaacgagac ctcatccatc ctggtggagt cagtgaccag gtcgtctaca
     8881 gagacctgct atagcgccat tcccaaggca tcctcggacg ccagcaaggt gacctctaag
     8941 ggggcagggc tctcaaaggc ctttgtgggc cagaagagtt ccttcctggt ggactgcagc
     9001 aaagctggct ccaacatgct gctgatcggg gtccatgggc ccaccacccc ctgcgaggag
     9061 gtctccatga agcatgtagg caaccagcaa tacaacgtca catacgtcgt caaggagagg
     9121 ggcgattatg tgctggctgt gaagtggggg gaggaacaca tccctggcag cccttttcat
     9181 gtcacagtgc cttaactcga gtctagaggg cccgatatct cgacaatcaa cctctggatt
     9241 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     9301 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     9361 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     9421 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     9481 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     9541 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     9601 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     9661 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     9721 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     9781 cgagtcggat ctccctttgg gccgcctccc cgcctggaaa cgggggaggc taactgaaac
     9841 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     9901 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     9961 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
    10021 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
    10081 gccctgccat agcagatctg cgcagctggg gctctagggg gtatccccac gcgccctgta
    10141 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
    10201 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
    10261 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc
    10321 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
    10381 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
    10441 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg
    10501 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
    10561 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
    10621 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
    10681 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
    10741 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
    10801 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
    10861 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
    10921 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
    10981 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
    11041 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
    11101 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
    11161 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
    11221 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
    11281 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
    11341 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
    11401 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
    11461 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
    11521 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
    11581 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
    11641 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
    11701 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
    11761 agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
    11821 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
    11881 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
    11941 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    12001 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    12061 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    12121 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    12181 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    12241 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    12301 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    12361 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    12421 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    12481 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    12541 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    12601 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    12661 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
    12721 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    12781 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    12841 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    12901 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
    12961 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    13021 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    13081 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    13141 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    13201 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    13261 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    13321 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    13381 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    13441 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    13501 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    13561 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    13621 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    13681 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    13741 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    13801 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    13861 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    13921 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    13981 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    14041 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    14101 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    14161 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    14221 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    14281 tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca
    14341 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
    14401 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttagg
//
