LOCUS       V030601                 8898 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030601
VERSION     V030601
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..5045
                     /label="Erbb3(NM_010153)"
                     /note="Erbb3(NM_010153)"
                     /gene="Erbb3"
     CDS             5064..5093
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             5112..5135
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(5155..5171)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    5182..5804
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(5953..6541)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    6870..6917
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(7149..7943)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(7978..8335)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(8337..8441)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      8468..8896
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gagtgcgatt gggactctgc aggtgctggg tttccttctc agcctggccc
     1081 ggggttccga gatgggcaac tctcaggcag tatgtcctgg gactctaaac gggctgagtg
     1141 tgaccggcga tgctgacaac cagtaccaga cactgtacaa actctatgag aagtgtgagg
     1201 tggtcatggg taacctggag attgtgctta cgggacacaa tgctgatctt tccttcctgc
     1261 aatggatccg agaagtgaca ggctatgtac tggtggccat gaatgaattc tctgtactgc
     1321 ccttacctaa cctccgagtg gtccggggaa cccaggtcta cgatgggaag tttgccatct
     1381 ttgtcatgtt gaactacaat accaactcca gccatgctct gcgccagctc cggttcactc
     1441 agcttactga gattctgtta gggggcgttt acattgagaa gaatgacaaa ctttgccaca
     1501 tggatacaat tgactggagg gacatcgtga gggttccaga cgctgagata gtggtgaaga
     1561 acaacggggg gaactgtcca ccctgtcacg aagtctgcaa ggggcgatgc tgggggcctg
     1621 gaccagaaga ctgccagata ttgaccaaga ccatctgtgc ccctcagtgt aacggtcgct
     1681 gcttcgggcc caatcctaac cagtgctgcc acgatgaatg tgcaggtggc tgctctggac
     1741 cccaggacac agattgcttc gcctgccgac acttcaatga cagtggtgcc tgtgtgccca
     1801 ggtgtccagc gccccttgtg tacaacaagc taacgttcca gcttgagccc aacccccata
     1861 tcaagtatca gtacggagga gtctgtgttg ccagttgtcc ccataacttt gtggtggatc
     1921 agacattttg tgtcagggct tgtcctgctg acaagatgga agtagataag aatggactca
     1981 agatgtgtga gccttgcaga gggctgtgcc caaaagcctg tgaggggacg ggctctggaa
     2041 gccgctacca gaccgtggac tctagcaata tcgatgggtt cgtgaactgt accaagatcc
     2101 tgggcaacct ggacttcctc atcactggcc tcaatggtga cccctggcac aagatccctg
     2161 cactggaccc ggaaaagctc aatgttttca ggacagtccg ggagattaca ggctacctaa
     2221 acatccagtc ctggccccct cacatgcaca acttcagtgt tttttccaac ctgacgacca
     2281 tcgggggcag aagcctctac aatcggggct tctccttgtt gatcatgaag aacttgaatg
     2341 tcacgtctct gggcttccgg tccctgaagg aaattagtgc tgggcgtgtc tacataagtg
     2401 ccaatcagca actttgttac caccactctc tgaactggac cagacttctg cgggggcccg
     2461 cagaggagag acttgacatc aagtacaacc ggcctctggg agaatgcgtg gcagagggca
     2521 aagtgtgtga tccactgtgc tcctctgggg gatgctgggg cccaggccct ggtcagtgct
     2581 tgtcttgtcg aaactacagc cgggaaggtg tctgtgtgac tcactgcaac gttctgcaag
     2641 gggaaccccg agagtttgtt catgaggctc attgcttctc ctgccatcca gaatgccagc
     2701 ccatggaggg caccagcacg tgcaatggct cgggctccga cgcttgtgct cgatgcgccc
     2761 attttcgtga tgggccccac tgtgtgaaca gctgccccca tggaatccta ggtgccaaag
     2821 gtccaatcta caaatatcca gatgctcaga atgagtgccg gccctgccac gagaactgca
     2881 cccaagggtg taagggacca gaactacaag actgtttagg ccaagcagag gtattaatga
     2941 gcaaaccaca cctggtcata gcggtgacag taggactgac tgtgatcttc ctgattctgg
     3001 gaggctcttt tctctattgg cgtggacgca ggattcagaa taaaagggct atgagacgct
     3061 acttggagcg gggtgagagc atcgagcctc tggacccaag cgagaaggca aacaaagtct
     3121 tggctagaat cttcaaagag acagagctga ggaaacttaa ggtgcttggc tctggtgtct
     3181 ttggaactgt acacaagggg atttggattc ccgagggtga atccatcaag attccagtct
     3241 gcattaaagt catcgaggac aagagtgggc ggcagagttt tcaggctgtg actgatcaca
     3301 tgctggccgt cggcagcctg gaccatgccc acattgtacg gctgctggga ctgtgcccag
     3361 ggtcatctct gcagcttgtc actcagtact tgcctctggg ctctctcctt gatcatgtaa
     3421 gacagcaccg tgagacactg ggaccacagc tgctgctcaa ctggggagta caaattgcca
     3481 agggtatgta ttacctcgag gaacacagca tggtgcatag ggaccttgcg ctccggaatg
     3541 tgatgcttaa gtcaccgagt caagtccagg tggcagattt tggtgtggct gacttgctgc
     3601 cgccagatga caagcagtta ctacacagtg aggccaagac tccaattaaa tggatggccc
     3661 ttgagagtat ccactttggg aaatacacac accagagtga tgtctggagt tacggtgtaa
     3721 ccgtttggga gttgatgacc ttcggggcag agccctacgc agggctacga ctggctgaaa
     3781 taccagacct gctggagaag ggagagcggt tagcacagcc ccagatctgc accattgacg
     3841 tctacatggt catggtcaag tgttggatga ttgacgagaa tattcgccca acctttaaag
     3901 aactggccaa tgagtttacc aggatggccc gggacccacc aaggtatctg gtcatcaaga
     3961 gagcgagtgg gcctggaata cctcctgcag cagagccatc tgctctgagc accaaagagt
     4021 tgcaggatgc agagctggag ccagacctgg acctcgacct agacgtggag gtagaagagg
     4081 agggcctggc gaccacactg ggttctgccc tcagcttgcc tacaggaacg cttacccggc
     4141 cacgtgggag ccagagtctt ttaagtcctt cgtctggata catgcccatg aaccagagca
     4201 accttgggga ggcttgtctg gattctgcgg ttttgggggg tcgcgaacag ttctcccgtc
     4261 ccatctctct gcacccgatc ccacgggggc gtcaaacgtc agagtcatca gagggccatg
     4321 tgacgggctc tgaggctgaa ctccaagaga gagtatcaat gtgtaggagc cggagccgga
     4381 gccggagccc acggccacgt ggggacagtg cctaccattc gcagcgacac agcctgctta
     4441 ctcccgtcac cccgctctcc ccaccagggt tagaggaaga ggatggcaat ggttatgtca
     4501 tgccagatac gcacctcaga ggtacatcct cttcccggga aggcaccctt tcgtcagtag
     4561 gtctcagttc tgtgctgggt accgaagagg aagatgaaga tgaggagtat gaatacatga
     4621 accggaagag gaggggtagc ccggctcggc cccccagacc tggttccctg gaagagctgg
     4681 gctatgagta catggatgtg ggttcagacc tcagtgcttc tctgggcagt acgcagagtt
     4741 gcccactcca tcccatggcc atcgtgccct ctgctggcac gactccagat gaggactatg
     4801 aatacatgaa ccgcaggcgt ggtgcgggcg gttccggagg ggattatgca gctatggggg
     4861 cctgcccagc agctgaacaa gggtatgagg aaatgcgagc tttccagggg cctggacatc
     4921 aagcccccca tgttcgttat gcccgcctca aaactctgcg tagtttagaa gccactgact
     4981 ccgcctttga caaccccgat tactggcata gcaggctttt ccctaaggct aacgcccaga
     5041 gaattacgcg tacgcggccg ctcgagcaga aactcatctc agaagaggat ctggcagcaa
     5101 atgatatcct ggattacaag gatgacgacg ataaggttta aacggccggc cgcggtcata
     5161 gctgtttcct gaacagatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     5221 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     5281 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     5341 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     5401 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     5461 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     5521 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     5581 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     5641 gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca
     5701 cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact
     5761 gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagcg
     5821 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5881 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5941 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6001 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6061 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6121 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6181 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6241 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6301 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6361 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6421 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6481 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6541 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6601 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6661 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc
     6721 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga
     6781 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg
     6841 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
     6901 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     6961 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     7021 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     7081 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     7141 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     7201 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     7261 atatcacggg tagccaacgc tatgtcctga tagcgatccg ccacacccag ccggccacag
     7321 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     7381 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
     7441 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     7501 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     7561 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     7621 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     7681 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     7741 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
     7801 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     7861 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     7921 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt
     7981 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     8041 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     8101 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     8161 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     8221 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     8281 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
     8341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8461 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8521 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8581 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8641 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     8701 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8761 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     8821 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     8881 aaatttaacg cgaatttt
//
